The 100 bp Ladder consists of 9 DNA fragments, namely 1500 bp, 1000 bp, 800 bp, 600 bp 500 bp, 400 bp, 300 bp, 200 bp, and 100 bp. This product contains DNA that already contains 1 x Loading Buffer Solution, can be taken as 5 μ L direct electrophoresis, very convenient to use; The amount of 500 bp DNA fragments during electrophoresis is approximately 150 ng,Displaying bright bands, the DNA content of other bands is approximately 50 ng.
Component
L665547 100 preps
L665547 500 preps
100 bp Ladder
500 µl
5×500 µl
Notes: 1. This product can be directly thawed and mixed for use without heating. 2. Please replace the electrophoresis buffer in time and use the newly prepared agarose gel to avoid affecting the electrophoresis results. 3. During DNA agarose gel electrophoresis, the purity of gel has a great impact on the clarity of DNA bands, so try to use high-quality agarose. The recommended concentration of gel is 2% -3%, and the voltage is 4-8 v/cm. 4. The concentration of gel is closely related to the separation performance of DNA fragments. The higher the concentration of gel, the better the separation performance of short DNA fragments; On the contrary, the smaller the gel concentration, the more conducive to the separation of long DNA fragments. 5. When used in combination with macromolecular dyes, it is recommended to appropriately reduce the amount of Marker or increase the amount of dye used.
Usage: Take 5 μ L is added to the sample adding hole of agarose gel (if the sample adding hole is wider than 6 mm, the sample loading amount must be increased appropriately) for electrophoresis.
