The 1 kb Ladder consists of 8 DNA fragments, namely 1 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, and 10 kb. This product is a DNA solution that already contains 1 x Loading Buffer, which can be taken as 5 μ Direct electrophoresis is very convenient to use; The amount of 5 kb DNA fragments during electrophoresis is about 150 ng, showing bright bands, while the amount of DNA in other bands is about 50 ng.
Component
L665551 100 preps
L665551 500 preps
1 kb Ladder
500 µl
5×500 µl
Notes: 1. This product can be directly thawed and mixed for use without heating. 2. Please replace the electrophoresis buffer in time and use the newly prepared agarose gel to avoid affecting the electrophoresis results. 3. During DNA agarose gel electrophoresis, the purity of gel has a great impact on the clarity of DNA bands, so try to use high-quality agarose. The recommended concentration of gel is 0.7% -1%, and the voltage is 4-8 v/cm. 4. The concentration of gel is closely related to the separation performance of DNA fragments. The higher the concentration of gel, the better the separation performance of short DNA fragments; On the contrary, the smaller the gel concentration, the more conducive to the separation of long DNA fragments. 5. When used in combination with macromolecular dyes, it is recommended to appropriately reduce the amount of Marker or increase the amount of dye used.
Usage: Take 5 μ L Add it into the sample adding hole of agarose gel (if the sample adding hole is wider than 6 mm, the sample loading amount must be increased appropriately), and conduct electrophoresis. Experimental results:
