2',7'-Dichlorodihydrofluorescein diacetate - 10mM in DMSO, high purity , CAS No.4091-99-0

Item Number

D423861

Grouped product items
SKU	Size	Availability	Price	Qty
D423861-1ml	1ml	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$62.90

Cell-permeable ROS probe

Basic Description

Synonyms	4091-99-0\|H2DCFDA\|2-(3,6-diacetoxy-2,7-dichloro-9h-xanthen-9-yl)benzoic acid\|2,7-Dichlorodihydrofluorescein diacetate\|Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-\|2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid\|2',7'-Dic
Specifications & Purity	10mM in DMSO
Storage Temp	Store at -80°C
Shipped In	Ice chest + Ice pads
Product Description	2′,7′-Dichlorofluorescin Diacetate is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. For maximum solubility in aqueous buffers, DCFH-DA can be directly disolved in 0.1 M Na2CO3 (5 mg/ml) and then immediately diluted with PBS (pH 7.2) to achieve the desired concentration or pH. Upon neutralization of the solution the concentration may not be greater than 1 mg/ml. We do not recommend storing the aqueous solution for more than one day.

Names and Identifiers

IUPAC Name	2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
INCHI	InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChi Key	PXEZTIWVRVSYOK-UHFFFAOYSA-N
Canonical SMILES	CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl
Isomeric SMILES	CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl
WGK Germany	3
PubChem CID	77718
Molecular Weight	487.286
Beilstein	4610273

Safety and Hazards(GHS)

Pictogram(s)	GHS07
Signal	Warning
Hazard Statements	H315:Causes skin irritation H319:Causes serious eye irritation H335:May cause respiratory irritation
Precautionary Statements	P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of water. P321:Specific treatment (see ... on this label). P405:Store locked up. P501:Dispose of contents/container to ... P264:Wash hands [and …] thoroughly after handling. P271:Use only outdoors or in a well-ventilated area. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P403+P233:Store in a well-ventilated place. Keep container tightly closed. P362+P364:Take off contaminated clothing and wash it before reuse. P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes. P337+P317:If eye irritation persists: Get medical help. P332+P317:If skin irritation occurs: Get medical help. P319:Get medical help if you feel unwell.
WGK Germany	3

Solution Calculators

Determine the necessary mass, volume, or concentration for preparing a solution.

Determine the dilution needed to prepare a stock solution.

Basic Description