2',7'-Dichlorodihydrofluorescein diacetate - ≥97%, high purity , CAS No.4091-99-0

Item Number

H131224

Grouped product items
SKU	Size	Availability	Price
H131224-10mg	10mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$23.90
H131224-50mg	50mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$43.90
H131224-100mg	100mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$58.90
H131224-250mg	250mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$132.90
H131224-1g	1g	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$477.90

Cell-permeable ROS probe

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Basic Description

Synonyms	4091-99-0\|H2DCFDA\|2-(3,6-diacetoxy-2,7-dichloro-9h-xanthen-9-yl)benzoic acid\|2,7-Dichlorodihydrofluorescein diacetate\|Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-\|2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid\|2',7'-Dic
Specifications & Purity	≥97%
Storage Temp	Store at -20°C,Argon charged
Shipped In	Ice chest + Ice pads
Product Description	2′,7′-Dichlorofluorescin Diacetate is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. For maximum solubility in aqueous buffers, DCFH-DA can be directly disolved in 0.1 M Na2CO3 (5 mg/ml) and then immediately diluted with PBS (pH 7.2) to achieve the desired concentration or pH. Upon neutralization of the solution the concentration may not be greater than 1 mg/ml. We do not recommend storing the aqueous solution for more than one day.

Names and Identifiers

IUPAC Name	2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
INCHI	InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChi Key	PXEZTIWVRVSYOK-UHFFFAOYSA-N
Canonical SMILES	CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl
Isomeric SMILES	CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl
WGK Germany	3
PubChem CID	77718
Molecular Weight	487.286
Beilstein	4610273

Certificates

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22 results found

Lot Number	Certificate Type	Date	Item
F2406232	Certificate of Analysis	May 24, 2024	H131224
F2406233	Certificate of Analysis	May 24, 2024	H131224
F2406237	Certificate of Analysis	May 24, 2024	H131224
F2406238	Certificate of Analysis	May 24, 2024	H131224
E2224174	Certificate of Analysis	Mar 20, 2024	H131224
H2328765	Certificate of Analysis	Aug 09, 2023	H131224
H2328647	Certificate of Analysis	Aug 09, 2023	H131224
H2328677	Certificate of Analysis	Aug 09, 2023	H131224
L2209541	Certificate of Analysis	Oct 10, 2022	H131224
L2209540	Certificate of Analysis	Oct 10, 2022	H131224
L2209528	Certificate of Analysis	Oct 10, 2022	H131224
L2209524	Certificate of Analysis	Oct 10, 2022	H131224
L2208528	Certificate of Analysis	Oct 10, 2022	H131224
C2215306	Certificate of Analysis	May 24, 2022	H131224
G2327066	Certificate of Analysis	Mar 15, 2022	H131224
I2221017	Certificate of Analysis	Mar 15, 2022	H131224
I2221018	Certificate of Analysis	Mar 15, 2022	H131224
E2224175	Certificate of Analysis	Mar 15, 2022	H131224
E2224172	Certificate of Analysis	Mar 15, 2022	H131224
E2224164	Certificate of Analysis	Mar 15, 2022	H131224
E2224155	Certificate of Analysis	Mar 15, 2022	H131224
C2205151	Certificate of Analysis	Mar 10, 2022	H131224

Solubility

Soluble in DMSO (25 mg/ml), 100% ethanol (25 mg/ml), DMF (25 mg/ml), and aqueous buffers (sparingly soluble).

Sensitivity

Air sensitive.Light sensitive.

Safety and Hazards(GHS)

Pictogram(s)	GHS07
Signal	Warning
Hazard Statements	H315:Causes skin irritation H319:Causes serious eye irritation H335:May cause respiratory irritation
Precautionary Statements	P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of water. P321:Specific treatment (see ... on this label). P405:Store locked up. P501:Dispose of contents/container to ... P264:Wash hands [and …] thoroughly after handling. P271:Use only outdoors or in a well-ventilated area. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P403+P233:Store in a well-ventilated place. Keep container tightly closed. P362+P364:Take off contaminated clothing and wash it before reuse. P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes. P337+P317:If eye irritation persists: Get medical help. P332+P317:If skin irritation occurs: Get medical help. P319:Get medical help if you feel unwell.
WGK Germany	3

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Basic Description