SpeciesGene IDAccessionECSourceLengthMWTag2019-nCoV43740578YP_009725301.13.4.22.69E. coli306aa~33.8kDa-About this proteinName2019-nCoV Main ProteaseSynonyms2019-nCoV Main Protease (Mpro)/ 3C-like Protease (3CLpro), Mpro/3CLpro, 2019-nCoV 3C-like Proteinase, main proteinase, 3C-like proteinase (NSP5), Coronavirus endopeptidase C30, chymotrypsin-like protease, coronavirus 3C-like protease, coronavirus M(pro), coronavirus main proteaseBackground2019-nCoV is a positive-sense single-stranded RNA virus and has high homology with SARS-CoV and MERS-CoV. After infecting host cells, the coronaviral ORF1a/b RNA is first translated with the help of host cells into two polyprotein precursors (PP1a and PP1ab) which are then cleaved by Mpro and papain like proteases to produce multiple nonstructural proteins. These nonstructural proteins are involved in the production of viral subgene RNA and four structural proteins (envelope/E protein, membrane/M protein, spike/S protein and nucleocapsid/N protein), which in turn complete the reproduction and release of the progeny virus. Since the Mpro protease plays a vital role in the life cycle of the virus and there is no homologous protein in the human body, the Mpro main protease is an ideal target for developing antiviral drugs. Meanwhile because Mpro is highly conservative among beta coronaviruses, the Mpro inhibitors selected have a broad-spectrum anti-coronavirus ability and may even be used in curing other animal diseases such as porcine coronavirus.Aladdin's 2019-nCoV Main Protease is purified using the PerfectProtein™ Platform developed by aladdin. It is recombinantly expressed in E. coli without any additional tags or amino acids, ensuring complete identity with the natural Main Protease in 2019-nCoV virus.s2019-nCoV inhibitor screening; enzyme activity or structural studiesPhysical AppearanceliquidBiological Activity≥300U/g or 300μmol/min/gUnit DefinitionOne unit is defined as the amount of enzyme that catalyzes the production of 1μmol of MCA-AVLQ per minute at 37℃ under pH7.0. Concentration~1mg/mlPurity≥ 95% by SDS-PAGE.Formulation500mM Tris, 150mM NaCl, 1mM EDTA, 50% Glycerol, pH7.3Recommended UsageFor inhibitor screening, add 0.2-1μg of this product generally in a reaction volume of 200μl.Amino Acid SequenceSGFRKMAFPS GKVEGCMVQV TCGTTTLNGL WLDDVVYCPR HVICTSEDML NPNYEDLLIR KSNHNFLVQA GNVQLRVIGH SMQNCVLKLK VDTANPKTPK YKFVRIQPGQ TFSVLACYNG SPSGVYQCAM RPNFTIKGSF LNGSCGSVGF NIDYDCVSFC YMHHMELPTG VHAGTDLEGN FYGPFVDRQT AQAAGTDTTI TVNVLAWLYA AVINGDRWFL NRFTTTLNDF NLVAMKYNYE PLTQDHVDIL GPLSAQTGIA VLDMCASLKE LLQNGMNGRT ILGSALLEDE FTPFDVVRQC SGVTFQNote
Precautions：
Before opening the tube, centrifuge briefly to collect liquid at the bottom of the tube.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
1. For the usage of this product, please refer to the manual of ’s Coronavirus Mpro/3CLpro Activity Fluorometric Assay Kit or other relevant literature, depending on the experimental purposes.2. Please refer to Figure 1 for the performance of this product in cleaving the CoV Main Protease Fluorogenic Substrate , MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2, into a fluorescent product with Ex/Em of 325/393nm.Figure 1. Digestion of MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 by different amounts of 2019-nCoV Main Protease (Mpro/3CLpro) (, P2320). This figure is for reference only, which may vary due to different experimental conditions.
