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P118471-5g

Available within 4-8 weeks(?)

$226.90

P118471-25g

25g

Available within 4-8 weeks(?)

$467.90

Basic Description

Synonyms	4-Nitrophenyl phosphate disodium salt hexahydrate \| p-nitrophenyl phosphate disodium salt hexahydrate \| 4-Nitrophenyl phosphate, disodium salt hexahydrate \| SY074980 \| 4-Nitrophenyl phosphate disodium salt hexahydrate (PNPP) \| Phosphatase \| FT-0766939 \| A
Specifications & Purity	for enzyme immunoassay, ≥99%
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Product Description	Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Names and Identifiers

IUPAC Name	disodium;(4-nitrophenyl) phosphate;hexahydrate
INCHI	InChI=1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;61H2/q;2+1;;;;;;/p-2
InChi Key	KAKKHKRHCKCAGH-UHFFFAOYSA-L
Canonical SMILES	C1=CC(=CC=C1[N+](=O)[O-])OP(=O)([O-])[O-].O.O.O.O.O.O.[Na+].[Na+]
Isomeric SMILES	C1=CC(=CC=C1[N+](=O)[O-])OP(=O)([O-])[O-].O.O.O.O.O.O.[Na+].[Na+]
WGK Germany	3
Alternate CAS	4264-83-9
PubChem CID	2723710
Molecular Weight	371.14
Beilstein	6237
Reaxy-Rn	5324661

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number	Certificate Type	Date	Item
G2418054	Certificate of Analysis	Jul 23, 2024	P118471
J2218174	Certificate of Analysis	Jul 09, 2024	P118471
J2218223	Certificate of Analysis	Jul 09, 2024	P118471
K1901036	Certificate of Analysis	May 09, 2023	P118471

Chemical and Physical Properties

Melt Point(°C)	300°C

Safety and Hazards(GHS)

Pictogram(s)	GHS08
Signal	Danger
Hazard Statements	H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled
Precautionary Statements	P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P501:Dispose of contents/container to ... P284:[In case of inadequate ventilation] Wear respiratory protection. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P342+P316:If experiencing respiratory symptoms: Get emergence medical help immediately.
WGK Germany	3
Reaxy-Rn	5324661

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Yan Li, Mingjie Chen, Huiming Liu, Dandan Zhang, Qing-Shan Shi, Xiao-Bao Xie, Yanzhu Guo. (2023) Antimicrobial Peptide-Inspired Design of Amino-Modified Lignin with Improved Antimicrobial Activities. BIOMACROMOLECULES, 24 (11): (5381–5393). [PMID:37908117] [10.1021/acs.biomac.3c00841]
2. Shijie Cao, Leyao Li, Hongwei An, Guobin Mao, Junbiao Dai, Yingxin Ma. (2023) Development of dual-mode ELISA based on ALP-catalyzed APP hydrolysis for IL-6 detection. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 236 (5): (115754). [PMID:37783051] [10.1016/j.jpba.2023.115754]
3. Shiyi Chen, Feiyang Tian, Juan Peng, Yan Meng, Yu Wang, Xiaogang He, Qian Wang, Ganghui Chu. (2023) Turning Zr(IV) into a phosphate ester mimetic enzyme via de novo synthesis for hydrolyzing organophosphorus warfare agents and rapid activity prediction by near-infrared spectroscopy. MICROCHEMICAL JOURNAL, 195 (109379). [PMID:8318735] [10.1016/j.microc.2023.109379]
4. Guobin Mao, Chunmin Qiu, Xing Luo, Yingqi Liang, Liqing Zhao, Weiren Huang, Junbiao Dai, Yingxin Ma. (2023) Synergistic effect-triggered fluorescence quenching enables rapid and sensitive detection of alkaline phosphatase. ANALYTICA CHIMICA ACTA, 1272 (341510). [PMID:37355336] [10.1016/j.aca.2023.341510]
5. Yuanhua Zhang, Yan Qiu, Lan Xia, Long Yuan, Hailong Wu, Rui Shi, Anqi Chen, Kai Zhou, Mingxue Zhou, Kailian Zhang, Chunxiang Zhang, Zhining Xia, Qifeng Fu. (2023) Surface Patterning Enhanced Visualization for Ultrasensitive Naked-Eye Detection and RGB-Based High-Density Information Storage. Advanced Optical Materials, 11 (14): (2300010). [PMID:] [10.1002/adom.202300010]
6. Luyu Wei, Fu Jiao, Zhilong Wang, Long Wu, Daming Dong, Yiping Chen. (2022) Enzyme-modulated photothermal immunoassay of chloramphenicol residues in milk and egg using a self-calibrated thermal imager. FOOD CHEMISTRY, 392 (133232). [PMID:35636182] [10.1016/j.foodchem.2022.133232]
7. LI Ying, YANG Dan, CHEN Bo, CAO Hai-Yan, ZHANG Qing-Feng. (2021) Antioxidative and digestive enzymes inhibitory activities of 27 edible plants. Food Science and Technology, 42 (e88621). [PMID:] [10.1590/fst.88621]
8. Honghong Rao, Huiyi Huang, Xinyuan Zhang, Xin Xue, Mingyue Luo, Haixia Liu, Zhonghua Xue. (2020) A simple thermometer-based photothermometric assay for alkaline phosphatase activity based on target-induced nanoprobe generation. NEW JOURNAL OF CHEMISTRY, 44 (41): (17753-17760). [PMID:] [10.1039/D0NJ03920A]
9. Mingyue Luo, Xin Xue, Honghong Rao, Hongqiang Wang, Xiuhui Liu, Xibin Zhou, Zhonghua Xue, Xiaoquan Lu. (2020) Simply converting color signal readout into thermal signal readout for breaking the color resolution limitation of colorimetric sensor. SENSORS AND ACTUATORS B-CHEMICAL, 309 (127707). [PMID:] [10.1016/j.snb.2020.127707]
10. Pengjuan Ni, Chuanxia Chen, Yuanyuan Jiang, Chenghui Zhang, Bo Wang, Yizhong Lu, Hua Wang. (2020) A fluorescent assay for alkaline phosphatase activity based on inner filter effect by in-situ formation of fluorescent azamonardine. SENSORS AND ACTUATORS B-CHEMICAL, 302 (127145). [PMID:] [10.1016/j.snb.2019.127145]
11. Yunlei Zhou, Fei Li, Hanwen Wu, Yan Chen, Huanshun Yin, Shiyun Ai, Jun Wang. (2019) Electrochemical aptasensing strategy for kanamycin detection based on target-triggered single-strand DNA adsorption on MoS2 nanosheets and enzymatic signal amplification. SENSORS AND ACTUATORS B-CHEMICAL, 296 (126664). [PMID:] [10.1016/j.snb.2019.126664]
12. Yaping Zhong, Fengfeng Xue, Peng Wei, Ruohan Li, Chunyan Cao, Tao Yi. (2018) Water-soluble MoS2 quantum dots for facile and sensitive fluorescence sensing of alkaline phosphatase activity in serum and live cells based on the inner filter effect. Nanoscale, 10 (45): (21298-21306). [PMID:30422141] [10.1039/C8NR05549A]
13. Dong He, Wen Luo, Zhiyuan Wang, Pengmei Lv, Zhenhong Yuan, Shaowei Huang, Jingliang Xv. (2017) Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability. ENZYME AND MICROBIAL TECHNOLOGY, 102 (35). [PMID:28465058] [10.1016/j.enzmictec.2017.03.010]
14. Haijian Liu, Ming Li, Yining Xia, Xueqin Ren. (2017) A Turn-On Fluorescent Sensor for Selective and Sensitive Detection of Alkaline Phosphatase Activity with Gold Nanoclusters Based on Inner Filter Effect. ACS Applied Materials & Interfaces, 9 (1): (120–126). [PMID:27966342] [10.1021/acsami.6b11920]
15. J. B. Fan, S. van de Velde, W. B. Sun, H. Yu, Y. L. Zhang, D. Wang. (2016) Enhanced toxicity of Cry2Ab with cantharidin and its analogues on Mythimna separata. JOURNAL OF APPLIED ENTOMOLOGY, 141 (5): (363-371). [PMID:] [10.1111/jen.12359]
16. Qingming Zhang, Xue Li, Bingchen Li, Huanshun Yin, Shiyun Ai. (2015) An electrochemical biosensor for the activity assay of polynucleotide kinase and inhibitor screening. Analytical Methods, 7 (23): (9984-9991). [PMID:] [10.1039/C5AY02295A]
17. Yunlei Zhou, Zhiqing Yang, Xue Li, Yue Wang, Huanshun Yin, Shiyun Ai. (2015) Electrochemical biosensor for detection of DNA hydroxymethylation based on glycosylation and alkaline phosphatase catalytic signal amplification. ELECTROCHIMICA ACTA, 174 (647). [PMID:] [10.1016/j.electacta.2015.06.043]
18. Mo Wang, Bingchen Li, Qing Zhou, Huanshun Yin, Yunlei Zhou, Shiyun Ai. (2015) Label-free, Ultrasensitive and Electrochemical Immunosensing Platform for microRNA Detection Using Anti-DNA:RNA Hybrid Antibody and Enzymatic Signal Amplification. ELECTROCHIMICA ACTA, 165 (130). [PMID:] [10.1016/j.electacta.2015.03.011]
19. Zhi-Gang Wang, Ji-Lin Zhang, De-Hui Sun, Jia-Zuan Ni. (2014) Novel Ti4+-chelated magnetic nanostructured affinity microspheres containing N-methylene phosphonic chitosan for highly selective enrichment and rapid separation of phosphopeptides. Journal of Materials Chemistry B, 2 (39): (6886-6892). [PMID:32261885] [10.1039/C4TB00916A]

References

1. Yan Li, Mingjie Chen, Huiming Liu, Dandan Zhang, Qing-Shan Shi, Xiao-Bao Xie, Yanzhu Guo. (2023) Antimicrobial Peptide-Inspired Design of Amino-Modified Lignin with Improved Antimicrobial Activities. BIOMACROMOLECULES, 24 (11): (5381–5393). [PMID:37908117] [10.1021/acs.biomac.3c00841]
2. Shijie Cao, Leyao Li, Hongwei An, Guobin Mao, Junbiao Dai, Yingxin Ma. (2023) Development of dual-mode ELISA based on ALP-catalyzed APP hydrolysis for IL-6 detection. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 236 (5): (115754). [PMID:37783051] [10.1016/j.jpba.2023.115754]
3. Shiyi Chen, Feiyang Tian, Juan Peng, Yan Meng, Yu Wang, Xiaogang He, Qian Wang, Ganghui Chu. (2023) Turning Zr(IV) into a phosphate ester mimetic enzyme via de novo synthesis for hydrolyzing organophosphorus warfare agents and rapid activity prediction by near-infrared spectroscopy. MICROCHEMICAL JOURNAL, 195 (109379). [PMID:8318735] [10.1016/j.microc.2023.109379]
4. Guobin Mao, Chunmin Qiu, Xing Luo, Yingqi Liang, Liqing Zhao, Weiren Huang, Junbiao Dai, Yingxin Ma. (2023) Synergistic effect-triggered fluorescence quenching enables rapid and sensitive detection of alkaline phosphatase. ANALYTICA CHIMICA ACTA, 1272 (341510). [PMID:37355336] [10.1016/j.aca.2023.341510]
5. Yuanhua Zhang, Yan Qiu, Lan Xia, Long Yuan, Hailong Wu, Rui Shi, Anqi Chen, Kai Zhou, Mingxue Zhou, Kailian Zhang, Chunxiang Zhang, Zhining Xia, Qifeng Fu. (2023) Surface Patterning Enhanced Visualization for Ultrasensitive Naked-Eye Detection and RGB-Based High-Density Information Storage. Advanced Optical Materials, 11 (14): (2300010). [PMID:] [10.1002/adom.202300010]
6. Luyu Wei, Fu Jiao, Zhilong Wang, Long Wu, Daming Dong, Yiping Chen. (2022) Enzyme-modulated photothermal immunoassay of chloramphenicol residues in milk and egg using a self-calibrated thermal imager. FOOD CHEMISTRY, 392 (133232). [PMID:35636182] [10.1016/j.foodchem.2022.133232]
7. LI Ying, YANG Dan, CHEN Bo, CAO Hai-Yan, ZHANG Qing-Feng. (2021) Antioxidative and digestive enzymes inhibitory activities of 27 edible plants. Food Science and Technology, 42 (e88621). [PMID:] [10.1590/fst.88621]
8. Honghong Rao, Huiyi Huang, Xinyuan Zhang, Xin Xue, Mingyue Luo, Haixia Liu, Zhonghua Xue. (2020) A simple thermometer-based photothermometric assay for alkaline phosphatase activity based on target-induced nanoprobe generation. NEW JOURNAL OF CHEMISTRY, 44 (41): (17753-17760). [PMID:] [10.1039/D0NJ03920A]
9. Mingyue Luo, Xin Xue, Honghong Rao, Hongqiang Wang, Xiuhui Liu, Xibin Zhou, Zhonghua Xue, Xiaoquan Lu. (2020) Simply converting color signal readout into thermal signal readout for breaking the color resolution limitation of colorimetric sensor. SENSORS AND ACTUATORS B-CHEMICAL, 309 (127707). [PMID:] [10.1016/j.snb.2020.127707]
10. Pengjuan Ni, Chuanxia Chen, Yuanyuan Jiang, Chenghui Zhang, Bo Wang, Yizhong Lu, Hua Wang. (2020) A fluorescent assay for alkaline phosphatase activity based on inner filter effect by in-situ formation of fluorescent azamonardine. SENSORS AND ACTUATORS B-CHEMICAL, 302 (127145). [PMID:] [10.1016/j.snb.2019.127145]
11. Yunlei Zhou, Fei Li, Hanwen Wu, Yan Chen, Huanshun Yin, Shiyun Ai, Jun Wang. (2019) Electrochemical aptasensing strategy for kanamycin detection based on target-triggered single-strand DNA adsorption on MoS2 nanosheets and enzymatic signal amplification. SENSORS AND ACTUATORS B-CHEMICAL, 296 (126664). [PMID:] [10.1016/j.snb.2019.126664]
12. Yaping Zhong, Fengfeng Xue, Peng Wei, Ruohan Li, Chunyan Cao, Tao Yi. (2018) Water-soluble MoS2 quantum dots for facile and sensitive fluorescence sensing of alkaline phosphatase activity in serum and live cells based on the inner filter effect. Nanoscale, 10 (45): (21298-21306). [PMID:30422141] [10.1039/C8NR05549A]
13. Dong He, Wen Luo, Zhiyuan Wang, Pengmei Lv, Zhenhong Yuan, Shaowei Huang, Jingliang Xv. (2017) Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability. ENZYME AND MICROBIAL TECHNOLOGY, 102 (35). [PMID:28465058] [10.1016/j.enzmictec.2017.03.010]
14. Haijian Liu, Ming Li, Yining Xia, Xueqin Ren. (2017) A Turn-On Fluorescent Sensor for Selective and Sensitive Detection of Alkaline Phosphatase Activity with Gold Nanoclusters Based on Inner Filter Effect. ACS Applied Materials & Interfaces, 9 (1): (120–126). [PMID:27966342] [10.1021/acsami.6b11920]
15. J. B. Fan, S. van de Velde, W. B. Sun, H. Yu, Y. L. Zhang, D. Wang. (2016) Enhanced toxicity of Cry2Ab with cantharidin and its analogues on Mythimna separata. JOURNAL OF APPLIED ENTOMOLOGY, 141 (5): (363-371). [PMID:] [10.1111/jen.12359]
16. Qingming Zhang, Xue Li, Bingchen Li, Huanshun Yin, Shiyun Ai. (2015) An electrochemical biosensor for the activity assay of polynucleotide kinase and inhibitor screening. Analytical Methods, 7 (23): (9984-9991). [PMID:] [10.1039/C5AY02295A]
17. Yunlei Zhou, Zhiqing Yang, Xue Li, Yue Wang, Huanshun Yin, Shiyun Ai. (2015) Electrochemical biosensor for detection of DNA hydroxymethylation based on glycosylation and alkaline phosphatase catalytic signal amplification. ELECTROCHIMICA ACTA, 174 (647). [PMID:] [10.1016/j.electacta.2015.06.043]
18. Mo Wang, Bingchen Li, Qing Zhou, Huanshun Yin, Yunlei Zhou, Shiyun Ai. (2015) Label-free, Ultrasensitive and Electrochemical Immunosensing Platform for microRNA Detection Using Anti-DNA:RNA Hybrid Antibody and Enzymatic Signal Amplification. ELECTROCHIMICA ACTA, 165 (130). [PMID:] [10.1016/j.electacta.2015.03.011]
19. Zhi-Gang Wang, Ji-Lin Zhang, De-Hui Sun, Jia-Zuan Ni. (2014) Novel Ti4+-chelated magnetic nanostructured affinity microspheres containing N-methylene phosphonic chitosan for highly selective enrichment and rapid separation of phosphopeptides. Journal of Materials Chemistry B, 2 (39): (6886-6892). [PMID:32261885] [10.1039/C4TB00916A]

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4-Nitrophenyl phosphate disodium salt hexahydrate - for enzyme immunoassay,≥99.0%, high purity , CAS No.333338-18-4