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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt - for molecular biology,≥99%, high purity , CAS No.6578-06-9

Item Number
B113076
Grouped product items
SKUSizeAvailabilityPrice Qty
B113076-100mg
100mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$58.90
B113076-500mg
500mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$265.90
B113076-1g
1g
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$334.90

Histochemical alkaline phosphatase substrate.

Basic Description

SynonymsAC-37057 | BCIP, 5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt | B1239 | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, tablet | 5-Bromo-4-chloro-3-indolyl phosphate, p-toluidinesalt
Specifications & Purityfor molecular biology, ≥99%
Biochemical and Physiological MechanismsChromogenic substrate for alkaline phosphatase. Often used in combination with nitro blue tetrazolium chloride (NBT) to amplify the sensitivity of staining.
Storage TempStore at -20°C,Argon charged
Shipped InIce chest + Ice pads
Gradefor molecular biology
NoteWherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Refer to SDS for further information Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Histochemical substrate for alkaline phosphatase.

Names and Identifiers

IUPAC Name (5-bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate;4-methylaniline
INCHI InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChi Key QEIFSLUFHRCVQL-UHFFFAOYSA-N
Canonical SMILES CC1=CC=C(C=C1)N.C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br
Isomeric SMILES CC1=CC=C(C=C1)N.C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br
WGK Germany 3
PubChem CID 81059
Molecular Weight 433.62

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Enter lot number to search for analytical chart:

Find and download the COA for your product by matching the lot number on the packaging.

7 results found

Lot NumberCertificate TypeDateItem
K2223165Certificate of AnalysisAug 23, 2024 B113076
K2223206Certificate of AnalysisAug 23, 2024 B113076
K2223207Certificate of AnalysisAug 23, 2024 B113076
B2225276Certificate of AnalysisDec 22, 2023 B113076
B2225279Certificate of AnalysisDec 22, 2023 B113076
B1911019Certificate of AnalysisSep 16, 2022 B113076
B2225312Certificate of AnalysisJan 18, 2022 B113076

Chemical and Physical Properties

Sensitivitylight sensitive,air sensitive,heat sensitive
Melt Point(°C)196-198°C

Safety and Hazards(GHS)

Pictogram(s) GHS07
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

WGK Germany 3

Related Documents

Citations of This Product(2)

1. Yihao Li, Nan Zhang, Jiarui Xu, Lijuan Liu, Xiaochuang Cao, Xianyong Lin, Chengliang Sun.  (2023)  Imazethapyr disrupts plant phosphorus homeostasis and acquisition strategies.  JOURNAL OF HAZARDOUS MATERIALS,  460  (132317).  [PMID:37619275] [10.1016/j.jhazmat.2023.132317]
2. Dan Sun, Weiqing Xu, Chongyang Liang, Wei Shi, Shuping Xu.  (2020)  Smart Surface-Enhanced Resonance Raman Scattering Nanoprobe for Monitoring Cellular Alkaline Phosphatase Activity during Osteogenic Differentiation.  ACS Sensors,  (6): (1758–1767).  [PMID:32388973] [10.1021/acssensors.0c00428]

References

1. Yihao Li, Nan Zhang, Jiarui Xu, Lijuan Liu, Xiaochuang Cao, Xianyong Lin, Chengliang Sun.  (2023)  Imazethapyr disrupts plant phosphorus homeostasis and acquisition strategies.  JOURNAL OF HAZARDOUS MATERIALS,  460  (132317).  [PMID:37619275] [10.1016/j.jhazmat.2023.132317]
2. Dan Sun, Weiqing Xu, Chongyang Liang, Wei Shi, Shuping Xu.  (2020)  Smart Surface-Enhanced Resonance Raman Scattering Nanoprobe for Monitoring Cellular Alkaline Phosphatase Activity during Osteogenic Differentiation.  ACS Sensors,  (6): (1758–1767).  [PMID:32388973] [10.1021/acssensors.0c00428]

Solution Calculators