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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt - for molecular biology,≥99%, high purity , CAS No.6578-06-9
Histochemical alkaline phosphatase substrate.
Basic Description Synonyms AC-37057 | BCIP, 5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt | B1239 | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, tablet | 5-Bromo-4-chloro-3-indolyl phosphate, p-toluidinesalt Specifications & Purity for molecular biology, ≥99% Storage Temp Store at -20°C,Argon charged Shipped In Ice chest + Ice pads Grade for molecular biology Product Description For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Names and Identifiers IUPAC Name (5-bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate;4-methylaniline INCHI InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3 InChi Key QEIFSLUFHRCVQL-UHFFFAOYSA-N Canonical SMILES CC1=CC=C(C=C1)N.C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br Isomeric SMILES CC1=CC=C(C=C1)N.C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br WGK Germany 3 PubChem CID 81059 Molecular Weight 433.62
Chemical and Physical Properties Sensitivity light sensitive,air sensitive,heat sensitive Melt Point(°C) 196-198°C
Safety and Hazards(GHS) Pictogram(s) GHS07 Hazard Statements H315: Causes skin irritation
H319: Causes serious eye irritation
H335: May cause respiratory irritation
Precautionary Statements P261: Avoid breathing dust/fume/gas/mist/vapors/spray.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.
WGK Germany 3
Citations of This Product 1. Yihao Li, Nan Zhang, Jiarui Xu, Lijuan Liu, Xiaochuang Cao, Xianyong Lin, Chengliang Sun. (2023) Imazethapyr disrupts plant phosphorus homeostasis and acquisition strategies. JOURNAL OF HAZARDOUS MATERIALS, 460 (132317). [PMID:37619275 ] 2. Dan Sun, Weiqing Xu, Chongyang Liang, Wei Shi, Shuping Xu. (2020) Smart Surface-Enhanced Resonance Raman Scattering Nanoprobe for Monitoring Cellular Alkaline Phosphatase Activity during Osteogenic Differentiation. ACS Sensors, 5 (6): (1758–1767). [PMID:32388973 ]
References 1. Yihao Li, Nan Zhang, Jiarui Xu, Lijuan Liu, Xiaochuang Cao, Xianyong Lin, Chengliang Sun. (2023) Imazethapyr disrupts plant phosphorus homeostasis and acquisition strategies. JOURNAL OF HAZARDOUS MATERIALS, 460 (132317). [PMID:37619275 ] 2. Dan Sun, Weiqing Xu, Chongyang Liang, Wei Shi, Shuping Xu. (2020) Smart Surface-Enhanced Resonance Raman Scattering Nanoprobe for Monitoring Cellular Alkaline Phosphatase Activity during Osteogenic Differentiation. ACS Sensors, 5 (6): (1758–1767). [PMID:32388973 ]
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