Amine-reactive fluorescent label. 6-FAM isomer. Polar tracer. Covalently labels peptides and proteins. 5-CFDA N-succinimidyl ester and 5-CFDA hydrolytic product.
Storage Temp
Store at 2-8°C
Shipped In
Wet ice
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.
1.Xiaowei Cao, Zhengqing Liu, Xiaogang Qin, Yuexing Gu, Yong Huang, Yayun Qian, Zhenguang Wang, Hongbo Li, Qunshan Zhu, Wei Wei. (2024) LoC-SERS platform for rapid and sensitive detection of colorectal cancer protein biomarkers. TALANTA, 270 (125563). [PMID:38134815][10.1016/j.talanta.2023.125563]
2.Min Li, Honglang Lu, Menghan Pi, Hui Zhou, Yufei Wang, Bin Yan, Wei Cui, Rong Ran. (2023) Water-Induced Phase Separation for Anti-Swelling Hydrogel Adhesives in Underwater Soft Electronics. Advanced Science, (2304780). [PMID:37750254][10.1002/advs.202304780]
3.Wang Youwei, Chen Bing, Fan Jiang, Wang Zhong. (2023) A simple and efficient strategy for trace detection of ferroptosis-related miRNAs based on novel hydrophobic paper-based plasmonic substrate and “inverse molecular sentinel (iMS)” nanoprobes. Frontiers in Bioengineering and Biotechnology, 11 [PMID:36937763][10.3389/fbioe.2023.1146111]
4.Weizhou Sha, Mingzhu Wang, Wei Wang, Zhi Yuan. (2022) pH-Responsive nanoprobes for tumour fluorescence imaging based on spirolactam rhodamine. Materials Chemistry Frontiers, 6 (19):(2869-2879). [PMID:][10.1039/D2QM00406B]
5.Xiao-Qin Yang, Li-Wen Bai, Yu Chen, Yue-Xiao Lin, Hua Xiang, Ting-Ting Xiang, Shuang-Xing Zhu, Li Zhou, Kai Li, Xinxiang Lei. (2022) Peptide probes with high affinity to target protein selection by phage display and characterization using biophysical approaches. NEW JOURNAL OF CHEMISTRY, 46 (21):(10299-10307). [PMID:][10.1039/D2NJ00621A]
7.Di Wang, Erjun Sun. (2020) Loading carboxyfluorescein into porous SiO2 spheres and UCNPs-cored porous SiO2 spheres: Emission turn-on sensing with a warning signal for tumor-biomarker 5-HIAA urine test. MICROPOROUS AND MESOPOROUS MATERIALS, 299 (110131). [PMID:][10.1016/j.micromeso.2020.110131]
8.Xiuxia Sun, Linyan Yao, Caihong Fu, Liting Luo, Jie Wang, Jianxi Xiao. (2019) Detection of target collagen peptides with single amino acid mutation using two fluorescent peptide probes. Journal of Materials Chemistry B, 7 (48):(7676-7682). [PMID:31566640][10.1039/C9TB00610A]
1.Xiaowei Cao, Zhengqing Liu, Xiaogang Qin, Yuexing Gu, Yong Huang, Yayun Qian, Zhenguang Wang, Hongbo Li, Qunshan Zhu, Wei Wei. (2024) LoC-SERS platform for rapid and sensitive detection of colorectal cancer protein biomarkers. TALANTA, 270 (125563). [PMID:38134815][10.1016/j.talanta.2023.125563]
2.Min Li, Honglang Lu, Menghan Pi, Hui Zhou, Yufei Wang, Bin Yan, Wei Cui, Rong Ran. (2023) Water-Induced Phase Separation for Anti-Swelling Hydrogel Adhesives in Underwater Soft Electronics. Advanced Science, (2304780). [PMID:37750254][10.1002/advs.202304780]
3.Wang Youwei, Chen Bing, Fan Jiang, Wang Zhong. (2023) A simple and efficient strategy for trace detection of ferroptosis-related miRNAs based on novel hydrophobic paper-based plasmonic substrate and “inverse molecular sentinel (iMS)” nanoprobes. Frontiers in Bioengineering and Biotechnology, 11 [PMID:36937763][10.3389/fbioe.2023.1146111]
4.Weizhou Sha, Mingzhu Wang, Wei Wang, Zhi Yuan. (2022) pH-Responsive nanoprobes for tumour fluorescence imaging based on spirolactam rhodamine. Materials Chemistry Frontiers, 6 (19):(2869-2879). [PMID:][10.1039/D2QM00406B]
5.Xiao-Qin Yang, Li-Wen Bai, Yu Chen, Yue-Xiao Lin, Hua Xiang, Ting-Ting Xiang, Shuang-Xing Zhu, Li Zhou, Kai Li, Xinxiang Lei. (2022) Peptide probes with high affinity to target protein selection by phage display and characterization using biophysical approaches. NEW JOURNAL OF CHEMISTRY, 46 (21):(10299-10307). [PMID:][10.1039/D2NJ00621A]
7.Di Wang, Erjun Sun. (2020) Loading carboxyfluorescein into porous SiO2 spheres and UCNPs-cored porous SiO2 spheres: Emission turn-on sensing with a warning signal for tumor-biomarker 5-HIAA urine test. MICROPOROUS AND MESOPOROUS MATERIALS, 299 (110131). [PMID:][10.1016/j.micromeso.2020.110131]
8.Xiuxia Sun, Linyan Yao, Caihong Fu, Liting Luo, Jie Wang, Jianxi Xiao. (2019) Detection of target collagen peptides with single amino acid mutation using two fluorescent peptide probes. Journal of Materials Chemistry B, 7 (48):(7676-7682). [PMID:31566640][10.1039/C9TB00610A]
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