The amine-reactive form of 5-CR 6G is used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red™ conjugates.
1.Henegariu O, Bray-Ward P, Ward DC. () Custom fluorescent-nucleotide synthesis as an alternative method for nucleic acid labeling.. Nat Biotechnol, [PMID:10700155][http://pubmed.ncbi.nlm.nih.gov/10700155]
2.Widengren J, Kudryavtsev V, Antonik M, Berger S, Gerken M, Seidel CA. () Single-molecule detection and identification of multiple species by multiparameter fluorescence detection.. Anal Chem, [PMID:16536444][http://pubmed.ncbi.nlm.nih.gov/16536444]
3.Bonnet G, Krichevsky O, Libchaber A. () Kinetics of conformational fluctuations in DNA hairpin-loops.. Proc Natl Acad Sci U S A, [PMID:9671724][http://pubmed.ncbi.nlm.nih.gov/9671724]
5.Talaga DS, Lau WL, Roder H, Tang J, Jia Y, DeGrado WF, Hochstrasser RM. () Dynamics and folding of single two-stranded coiled-coil peptides studied by fluorescent energy transfer confocal microscopy.. Proc Natl Acad Sci U S A, [PMID:11087856][http://pubmed.ncbi.nlm.nih.gov/11087856]
6.Ogawa M, Kosaka N, Choyke PL, Kobayashi H,. () H-type dimer formation of fluorophores: a mechanism for activatable, in vivo optical molecular imaging.. ACS Chem Biol, [PMID:19480464][http://pubmed.ncbi.nlm.nih.gov/19480464]
7.Castro A, Williams JG. () Single-molecule detection of specific nucleic acid sequences in unamplified genomic DNA.. Anal Chem, [PMID:9322430][http://pubmed.ncbi.nlm.nih.gov/9322430]
8.Hung SC, Ju J, Mathies RA, Glazer AN. () Energy transfer primers with 5- or 6-carboxyrhodamine-6G as acceptor chromophores.. Anal Biochem, [PMID:8660606][http://pubmed.ncbi.nlm.nih.gov/8660606]
10.Longmire MR, Ogawa M, Hama Y, Kosaka N, Regino CA, Choyke PL, Kobayashi H,. () Determination of optimal rhodamine fluorophore for in vivo optical imaging.. Bioconjug Chem, [PMID:18610943][http://pubmed.ncbi.nlm.nih.gov/18610943]
Solution Calculators
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.