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6-Carboxyfluorescein - 95%, high purity , CAS No.3301-79-9

  • ≥95%
Item Number
C105327
Grouped product items
SKUSizeAvailabilityPrice Qty
C105327-25mg
25mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$27.90
C105327-100mg
100mg
In stock
$90.90
C105327-250mg
250mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$204.90
C105327-500mg
500mg
In stock
$247.90
C105327-1g
1g
In stock
$446.90
C105327-5g
5g
In stock
$2,008.90
C105327-25g
25g
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$9,039.90

Amine-reactive fluorescent label

Basic Description

Synonyms6-FAM | FLUOS | AS-47134 | AC-28936 | CHEBI:39073 | J-100061 | 6-FAM | F12261 | 1,5-diamino-4,8-dihydroxy-anthracene-9,10-dione | SCHEMBL20833 | SY057358 | Quinaldinium, 4-amino-1-dodecyl-, acetate | 5(6)-carboxy fluorescein | HY-66021 | Spiro[isobenzofur
Specifications & Purity≥95%
Biochemical and Physiological MechanismsAmine-reactive fluorescent label. 5-FAM isomer. 6-CFDA and 6-CFDA N -succinimidyl ester hydrolytic product. Polar tracer. Covalently labels peptides and proteins. Deprotonates below pH 7 resulting in reduced fluoresc
Storage TempProtected from light,Store at -20°C
Shipped InIce chest + Ice pads
NoteWherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

6-Carboxyfluorescein, or 6-FAM is a fluorescent dye primarily utilized in labeling nucleotides and nucleic acids. Also useful as a starting material for the synthesis of other fluorescein-derived reagents.

Names and Identifiers

IUPAC Name 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
INCHI InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)
InChi Key BZTDTCNHAFUJOG-UHFFFAOYSA-N
Canonical SMILES C1=CC2=C(C=C1C(=O)O)C3(C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O)OC2=O
Isomeric SMILES C1=CC2=C(C=C1C(=O)O)C3(C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O)OC2=O
WGK Germany 3
PubChem CID 76806
Molecular Weight 376.32
Beilstein 54341
Reaxy-Rn 54341

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

10 results found

Lot NumberCertificate TypeDateItem
G2403242Certificate of AnalysisApr 13, 2024 C105327
L1910174Certificate of AnalysisSep 11, 2023 C105327
C2404222Certificate of AnalysisJun 15, 2022 C105327
G2222137Certificate of AnalysisJun 15, 2022 C105327
G2222146Certificate of AnalysisJun 15, 2022 C105327
G2222157Certificate of AnalysisJun 15, 2022 C105327
G2222158Certificate of AnalysisJun 15, 2022 C105327
G2222202Certificate of AnalysisJun 15, 2022 C105327
H2307931Certificate of AnalysisJun 15, 2022 C105327
L2306051Certificate of AnalysisJun 15, 2022 C105327

Chemical and Physical Properties

Solubility0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange
SensitivityLight sensitive.
Melt Point(°C)150°C

Safety and Hazards(GHS)

Pictogram(s) GHS07
Signal Warning
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P271:Use only outdoors or in a well-ventilated area.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P403+P233:Store in a well-ventilated place. Keep container tightly closed.

P362+P364:Take off contaminated clothing and wash it before reuse.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P337+P317:If eye irritation persists: Get medical help.

P332+P317:If skin irritation occurs: Get medical help.

P319:Get medical help if you feel unwell.

WGK Germany 3
Reaxy-Rn 54341

Related Documents

Citations of This Product(12)

1. Qiang Chen, Lili Zheng, Xiaoqin Deng, Menghan Zhang, Wendi Han, Zhengjun Huang, Chenfang Miao, Shaohuang Weng.  (2023)  A fluorescence biosensor for tyrosinase activity analysis based on silicon-doped carbon quantum dots.  CHEMICAL & PHARMACEUTICAL BULLETIN,    (4):   [PMID:37704432] [10.1248/cpb.c23-00410]
2. Mingliang Mei, Lixuan Mu, Sen Liang, Yuan Wang, Guangwei She, Wensheng Shi.  (2021)  A general configurational strategy to quencher-free aptasensors.  BIOSENSORS & BIOELECTRONICS,  178  (113025).  [PMID:33529860] [10.1016/j.bios.2021.113025]
3. Ruicong Ma, Gentao Li, Xue Wang, Yajuan Bi, Youcai Zhang.  (2021)  Inhibitory effect of sixteen pharmaceutical excipients on six major organic cation and anion uptake transporters.  XENOBIOTICA,  51  (1): (95-104).  [PMID:32544367] [10.1080/00498254.2020.1783720]
4. Li Caiyu, Wang Xue, Bi Yajuan, Yu Heshui, Wei Jing, Zhang Yi, Han Lifeng, Zhang Youcai.  (2020)  Potent Inhibitors of Organic Anion Transporters 1 and 3 From Natural Compounds and Their Protective Effect on Aristolochic Acid Nephropathy.  TOXICOLOGICAL SCIENCES,  175  (2): (279-291).  [PMID:32159797] [10.1093/toxsci/kfaa033]
5. Xiaobo Fan, Wei Xu, Wei Gao, Xinglu Jiang, Guoqiu Wu.  (2020)  A facile method to classify clinic isolates with a turn-off sensor array based on graphene oxide and antimicrobial peptides.  SENSORS AND ACTUATORS B-CHEMICAL,  307  (127607).  [PMID:] [10.1016/j.snb.2019.127607]
6. Peter Yaro, Jing Nie, Mingcheng Xu, Kui Zeng, Houhong He, Jianbiao Yao, Ruwei Wang, Su Zeng.  (2019)  Influence of organic anion transporter 1/3 on the pharmacokinetics and renal excretion of ginkgolides and bilobalide.  JOURNAL OF ETHNOPHARMACOLOGY,  243  (112098).  [PMID:31325605] [10.1016/j.jep.2019.112098]
7. Li Xue, Wang Xue, Li Caiyu, Khutsishvili Manana, Fayvush George, Atha Daniel, Zhang Youcai, Borris Robert P..  (2019)  Unusual Flavones from Primula macrocalyx as Inhibitors of OAT1 and OAT3 and as Antifungal Agents against Candida rugosa.  Scientific Reports,  (1): (1-9).  [PMID:31239507] [10.1038/s41598-019-45728-5]
8. Yilin Qiao, Xueling Liu, Xue Li, Xue Wang, Caiyu Li, Manana Khutsishvili, Valida Alizade, Daniel Atha, Youcai Zhang, Robert P. Borris.  (2019)  Biflavonoids from Juniperus oblonga inhibit organic anion transporter 3.  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,  509  (931).  [PMID:30648554] [10.1016/j.bbrc.2019.01.039]
9. Xu Pang, Li-Ming Wang, You-Cai Zhang, Li-Ping Kang, He-Shui Yu, Guan-Wei Fan, Li-Feng Han.  (2019)  New anthraquinone and eurotinone analogue from the seeds of Senna obtusifolia and their inhibitory effects on human organic anion transporters 1 and 3.  NATURAL PRODUCT RESEARCH,  33  (23): (3409-3416).  [PMID:29863900] [10.1080/14786419.2018.1480621]
10. Wang Xue, Han Lifeng, Li Gentao, Peng Wei, Gao Xiumei, Klaassen Curtis D, Fan Guanwei, Zhang Youcai.  (2017)  From the Cover: Identification of Natural Products as Inhibitors of Human Organic Anion Transporters (OAT1 and OAT3) and Their Protective Effect on Mercury-Induced Toxicity.  TOXICOLOGICAL SCIENCES,  161  (2): (321-334).  [PMID:29045746] [10.1093/toxsci/kfx216]
11. Hang Lu, Zhiqiang Lu, Xue Li, Gentao Li, Yilin Qiao, Robert P. Borris, Youcai Zhang.  (2017)  Interactions of 172 plant extracts with human organic anion transporter 1 (SLC22A6) and 3 (SLC22A8): a study on herb-drug interactions.  PeerJ,  (e3333).  [PMID:28560096] [10.7717/peerj.3333]
12. Li X, Qiao Y, Wang X, Ma R, Li T, Zhang Y, Borris RP..  (2019)  Dihydrophenanthrenes from Juncus effusus as Inhibitors of OAT1 and OAT3..  J Nat Prod,  82  (4): (832-839).  [PMID:30892891] [10.1021/acs.jnatprod.8b00888]

References

1. Qiang Chen, Lili Zheng, Xiaoqin Deng, Menghan Zhang, Wendi Han, Zhengjun Huang, Chenfang Miao, Shaohuang Weng.  (2023)  A fluorescence biosensor for tyrosinase activity analysis based on silicon-doped carbon quantum dots.  CHEMICAL & PHARMACEUTICAL BULLETIN,    (4):   [PMID:37704432] [10.1248/cpb.c23-00410]
2. Mingliang Mei, Lixuan Mu, Sen Liang, Yuan Wang, Guangwei She, Wensheng Shi.  (2021)  A general configurational strategy to quencher-free aptasensors.  BIOSENSORS & BIOELECTRONICS,  178  (113025).  [PMID:33529860] [10.1016/j.bios.2021.113025]
3. Ruicong Ma, Gentao Li, Xue Wang, Yajuan Bi, Youcai Zhang.  (2021)  Inhibitory effect of sixteen pharmaceutical excipients on six major organic cation and anion uptake transporters.  XENOBIOTICA,  51  (1): (95-104).  [PMID:32544367] [10.1080/00498254.2020.1783720]
4. Li Caiyu, Wang Xue, Bi Yajuan, Yu Heshui, Wei Jing, Zhang Yi, Han Lifeng, Zhang Youcai.  (2020)  Potent Inhibitors of Organic Anion Transporters 1 and 3 From Natural Compounds and Their Protective Effect on Aristolochic Acid Nephropathy.  TOXICOLOGICAL SCIENCES,  175  (2): (279-291).  [PMID:32159797] [10.1093/toxsci/kfaa033]
5. Xiaobo Fan, Wei Xu, Wei Gao, Xinglu Jiang, Guoqiu Wu.  (2020)  A facile method to classify clinic isolates with a turn-off sensor array based on graphene oxide and antimicrobial peptides.  SENSORS AND ACTUATORS B-CHEMICAL,  307  (127607).  [PMID:] [10.1016/j.snb.2019.127607]
6. Peter Yaro, Jing Nie, Mingcheng Xu, Kui Zeng, Houhong He, Jianbiao Yao, Ruwei Wang, Su Zeng.  (2019)  Influence of organic anion transporter 1/3 on the pharmacokinetics and renal excretion of ginkgolides and bilobalide.  JOURNAL OF ETHNOPHARMACOLOGY,  243  (112098).  [PMID:31325605] [10.1016/j.jep.2019.112098]
7. Li Xue, Wang Xue, Li Caiyu, Khutsishvili Manana, Fayvush George, Atha Daniel, Zhang Youcai, Borris Robert P..  (2019)  Unusual Flavones from Primula macrocalyx as Inhibitors of OAT1 and OAT3 and as Antifungal Agents against Candida rugosa.  Scientific Reports,  (1): (1-9).  [PMID:31239507] [10.1038/s41598-019-45728-5]
8. Yilin Qiao, Xueling Liu, Xue Li, Xue Wang, Caiyu Li, Manana Khutsishvili, Valida Alizade, Daniel Atha, Youcai Zhang, Robert P. Borris.  (2019)  Biflavonoids from Juniperus oblonga inhibit organic anion transporter 3.  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,  509  (931).  [PMID:30648554] [10.1016/j.bbrc.2019.01.039]
9. Xu Pang, Li-Ming Wang, You-Cai Zhang, Li-Ping Kang, He-Shui Yu, Guan-Wei Fan, Li-Feng Han.  (2019)  New anthraquinone and eurotinone analogue from the seeds of Senna obtusifolia and their inhibitory effects on human organic anion transporters 1 and 3.  NATURAL PRODUCT RESEARCH,  33  (23): (3409-3416).  [PMID:29863900] [10.1080/14786419.2018.1480621]
10. Wang Xue, Han Lifeng, Li Gentao, Peng Wei, Gao Xiumei, Klaassen Curtis D, Fan Guanwei, Zhang Youcai.  (2017)  From the Cover: Identification of Natural Products as Inhibitors of Human Organic Anion Transporters (OAT1 and OAT3) and Their Protective Effect on Mercury-Induced Toxicity.  TOXICOLOGICAL SCIENCES,  161  (2): (321-334).  [PMID:29045746] [10.1093/toxsci/kfx216]
11. Hang Lu, Zhiqiang Lu, Xue Li, Gentao Li, Yilin Qiao, Robert P. Borris, Youcai Zhang.  (2017)  Interactions of 172 plant extracts with human organic anion transporter 1 (SLC22A6) and 3 (SLC22A8): a study on herb-drug interactions.  PeerJ,  (e3333).  [PMID:28560096] [10.7717/peerj.3333]
12. Li X, Qiao Y, Wang X, Ma R, Li T, Zhang Y, Borris RP..  (2019)  Dihydrophenanthrenes from Juncus effusus as Inhibitors of OAT1 and OAT3..  J Nat Prod,  82  (4): (832-839).  [PMID:30892891] [10.1021/acs.jnatprod.8b00888]

Solution Calculators