The 6X His Peptide is commonly used for the competitive elution of His tag fusion proteins bound to His antibodies. It can be used to elute His tag fusion proteins from Anti-His Magnetic Beads and conventional Anti-His antibodies during immunoprecipitation.This product competitively elutes the 6X His fusion protein. The eluted protein sample does not contain the light and heavy chains of Anti-His antibody, thus effectively eliminating the interference of anibodies with downstream Western blot analysis.The amino acid sequence of 6X His Peptide is His-His-His-His-His-His (HHHHHH).The main parameters of 6X His Peptide are as follows
Precautions：
The solution of this product should be aliquoted and stored at -20℃ or lower temperatures to avoid repeated freeze-thaw cycles.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
1. For 25mg provided in powder form, centrifuge at 8,000-12,000×g for 10-30 seconds before opening to collect the powder at the bottom of the tube. Dissolve it in TBS by gently pipetting or shaking. Do not vortex vigorously to avoid denaturation and inactivation of the peptide.2. P9811-1mg and P9811-5mg can be directly used for preparing elution buffer. 3. Competitive elution with the 6X His Peptide: This elution method is non-denaturing, with high elution efficiency, and the eluted sample does not contain the light and heavy chains of His antibodies.a. Preparation of 6X His tag elution buffer: Based on the required amount of elution buffer, dilute an appropriate amount of 6X His Peptide stock solution with TBS to a final concentration of 150μg/ml.b. Add 100μl of 6X His tag elution buffer (150μg/ml) to each immunoprecipitated sample, and incubate on ice or at 4℃ for 30 minutes to 2 hours with shaking. In order to improve the elution efficiency, the incubation time can be extended or the elution can be repeated. The volume of the 6X His tag elution buffer is generally 5 times that of the bead or gel suspension.c. Centrifuge at 6000×g for 30 seconds at 4℃, and carefully transfer the supernatant to a new tube. The supernatant contains the 6X His fusion protein and its protein complexes. Be careful not to touch the resin when taking the supernatant. 4. Store the eluted 6X His fusion protein at 4℃ for immediate use, or at -20℃ for long-term storage.Related Products:产品编号产品名称包装P9801-1mg3X Flag Peptide5mg/ml×0.2mlP9801-25mg3X Flag Peptide25mgP9801-5mg3X Flag Peptide5mg/ml×1mlP9805-1mgc-Myc Peptide5mg/ml×0.2mlP9805-25mgc-Myc Peptide25mgP9805-5mgc-Myc Peptide5mg/ml×1mlP9808-1mgHA Peptide5mg/ml×0.2mlP9808-25mgHA Peptide25mgP9808-5mgHA Peptide5mg/ml×1mlP9811-1mg6X His Peptide5mg/ml×0.2ml25mg6X His Peptide25mgP9811-5mg6X His Peptide5mg/ml×1mlP9813-1mgV5 Tag Peptide5mg/ml×0.2mlP9813-25mgV5 Tag Peptide25mgP9813-5mgV5 Tag Peptide5mg/ml×1mlP9816-1mgS Tag Peptide5mg/ml×0.2ml25mgS Tag Peptide25mgP9816-5mgS Tag Peptide5mg/ml×1mlP9819-1mgT7 Tag Peptide 5mg/ml×0.2mlP9819-25mgT7 Tag Peptide25mgP9819-5mgT7 Tag Peptide5mg/ml×1mlP9822-1mgVSV-G Tag Peptide 5mg/ml×0.2mlP9822-25mgVSV-G Tag Peptide25mgP9822-5mgVSV-G Tag Peptide5mg/ml×1ml
