Alcohol Dehydrogenase from Saccharomyces cerevisiae - ≥300 units/mg protein, high purity , CAS No.9031-72-5

Item Number

A124756

• Specifications & Purity: ≥300 units/mg protein
• Storage Temp: Store at -20°C
• Shipped In: Dry ice

Product Description

Protein Accession Number: P00330

CATH Classification (v. 3.2.0):

Class: Alpha Beta

Architecture: Alpha-Beta Complex and 3-Layer (aba) Sandwich

Topology: Quinone Oxidoreductase; Chain A, domain 1 and Rossmann fold

Molecular Weight: 146.8 kDa (Theoretical)

Optimal pH: 5.4 (Shore and Theorell 1966)

Isoelectric Point: 6.23 (Theoretical)

Extinction Coefficient:

189,320 cm-1 M-1 (Theoretical)

E1%,280 = 12.89 (Theoretical)

Activators:

Sulfhydryl activating reagents

Mercaptoethanol

Dithiothreitol

Cysteine

Heavy metal chelating agents

(White and White 1997)

Inhibitors:

Heavy metals and -SH reagents

Thiourea

Purine and pyrimidine derivatives

Chloroethanol and fluoroethanol

N-alkylmaleimides

Iodoacetamide

1,10-phenanthroline

8-hydroxyquinoline

Beta-NAD analogs

         (White and White 1997)

Applications:

Enzymatic determination of primary alcohols, and aldehydes

Synthesis of chiral compounds

Spectrophotometric assay of plasmalogenase

Enzymatic catalysis in organic solvents

Studies of NAD+, NADH, NADP+, and NADPH