A549 Whole Cell Lysateused for WB various applications
Grade
Azide Free, Carrier Free
Product Description
The A549 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
A549 cells are used as an in vitro model for a type II pulmonary epithelial cell model for drug metabolism. Cells are cytokeratin positive by immunoperoxidase staining. This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male (PubMed 4357758). Further studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway (PubMed 175022).
Specifications & Purity
Carrier Free, Azide Free, 200μg, Mycoplasma free
Images
A549 Whole Cell Lysate (cl155906) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
A549 Whole Cell Lysate (cl155906) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
A549 Whole Cell Lysate (cl155906) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 58 kDa
Product Specifications
Application
WB
Form
Lyophilized
Concentration
200μg, Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white po
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
Certificates
Certificate of Analysis(COA)
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ZJ23F0500182