Acetyl-Coenzyme A, trilithium saltAcetyl-Coenzyme A is used to assay CAT enzyme activity in cell extracts using radioisotopes. CAT enzyme activity in cell extracts catalyzes the transfer of acetyl groups from acetylcoenzyme A to chloramphenicol. A number of assays have been developed to measure CAT activity in cell extracts. Acetyl-Coenzyme A has also been used to determine citrate synthase activity.
1.Liu Xinning, Liu Yuanchang, Zhao Guangjian, Zhang Yidan, Liu Lu, Wang Juan, Wang Yifan, Zhang Siyu, Li Xin, Guo Dongliang, Wang Peng, Xu Ximing. (2021) Biochemical Characterization of Arylamine N-acetyltransferases From Vibrio vulnificus. Frontiers in Microbiology, 11 [PMID:33537010][10.3389/fmicb.2020.595083]
References
1.Eileen B Ekstrom,François M M Morel,Janina M Benoit. (2003-09-06) Mercury methylation independent of the acetyl-coenzyme A pathway in sulfate-reducing bacteria.. Applied and environmental microbiology, 69 ((9)):(5414-5422). [PMID:12957930]
2.Giosalba Burgio,Maria C Onorati,Davide F V Corona. (2010-05-25) Chromatin remodeling regulation by small molecules and metabolites.. Biochimica et biophysica acta, 1799 ((10-12)):(671-680). [PMID:20493981]
3.Morten Scheibye-Knudsen,Sarah J Mitchell,Evandro F Fang,Teruaki Iyama,Theresa Ward,James Wang,Christopher A Dunn,Nagendra Singh,Sebastian Veith,Md Mahdi Hasan-Olive,Aswin Mangerich,Mark A Wilson,Mark P Mattson,Linda H Bergersen,Victoria C Cogger,Alessandra Warren,David G Le Couteur,Ruin Moaddel,David M Wilson,Deborah L Croteau,Rafael de Cabo,Vilhelm A Bohr. (2014-12-03) A high-fat diet and NAD(+) activate Sirt1 to rescue premature aging in cockayne syndrome.. Cell metabolism, 20 ((5)):(840-855). [PMID:25440059]
5.Liu Xinning, Liu Yuanchang, Zhao Guangjian, Zhang Yidan, Liu Lu, Wang Juan, Wang Yifan, Zhang Siyu, Li Xin, Guo Dongliang, Wang Peng, Xu Ximing. (2021) Biochemical Characterization of Arylamine N-acetyltransferases From Vibrio vulnificus. Frontiers in Microbiology, 11 [PMID:33537010][10.3389/fmicb.2020.595083]
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