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Specifications & Purity | sufficient for 100colorimetrictests |
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Storage Temp | Store at -20°C |
Shipped In | Ice chest + Ice pads |
Product Description | Description Aconitase (Aconitate Hydratase) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich′s ataxia and diabetes. Preparation instructions Suitable for aconitase activity determination in biological samples (e.g. cell lysate, tissue homogenate, serum, etc.) Principle The Aconitase Assay Kit measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption m |
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