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Acridine Orange hemi(zinc chloride) salt - for electrophoresis, high purity , CAS No.10127-02-3

22 Citations

for electrophoresis

CAS#: 10127-02-3
Formula: C₁₇H₂₀ClN₃ · HCl · 1/2ZnCl₂
Molecular Weight: 369.96
Beilstein Registry Number: 3734978
EC Number: 233-353-6
MDL number: MFCD00081043
PubChem CID: 159263

Item Number

A100495

Grouped product items
SKU	Size	Availability	Price
A100495-250mg	250mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$9.90
A100495-1g	1g	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$11.90
A100495-5g	5g	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$22.90
A100495-25g	25g	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$45.90

Cell-permeable metachromatic fluorescent cationic DNA and RNA dye; RNA polymerase inhibitor.

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Basic Description

Synonyms	C.I. 46005 \| Euchrysin GNY \| Euchrysine 3RX \| AKOS027250830 \| N,N,N inverted exclamation marka,N inverted exclamation marka-Tetramethylacridine-3,6-diamine monohydrochloride compd. with zinc dichloride \| BasicOrange14 \| DTXSID20906035 \| N,N,N',N'-Tetramet
Specifications & Purity	for electrophoresis
Biochemical and Physiological Mechanisms	Cell-permeable metachromatic fluorescent cationic dye that stains DNA and RNA. Also an inhibitor of RNA polymerase.
Storage Temp	Store at 2-8°C,Argon charged
Shipped In	Wet ice
Grade	for electrophoresis
Note	Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Toxic, refer to SDS for further information . Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description	Acridine Orange hemi (zinc chloride) salt is a cell-permeable metachromatic fluorescent dye that stains DNA and RNA. This dye is also an inhibitor of RNA polymerase. Fluorescent dye that stains DNA and RNA Application Suitable for detection of nucleic acids separated by gel electrophoresis fluorescence and epifluorescence microscopy analysis of mitochondria and lysosomes by flow cytometry DNA staining in apoptosis studies Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Names and Identifiers

IUPAC Name	zinc;3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine;dichloride;hydrochloride
INCHI	InChI=1S/C17H19N3.3ClH.Zn/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;/h5-11H,1-4H3;3*1H;/q;;;;+2/p-2
InChi Key	VADJQOXWNSPOQA-UHFFFAOYSA-L
Canonical SMILES	CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C.Cl.[Cl-].[Cl-].[Zn+2]
Isomeric SMILES	CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C.Cl.[Cl-].[Cl-].[Zn+2]
WGK Germany	3
PubChem CID	159263
Molecular Weight	369.96
Beilstein	3734978
Reaxy-Rn	10510633

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

5 results found

Lot Number	Certificate Type	Date	Item
F1529001	Certificate of Analysis	Mar 10, 2023	A100495
G2216055	Certificate of Analysis	Jun 15, 2022	A100495
G2216079	Certificate of Analysis	Jun 15, 2022	A100495
I2205456	Certificate of Analysis	Jun 15, 2022	A100495
F2207282	Certificate of Analysis	Jul 30, 2021	A100495

Chemical and Physical Properties

Solubility	Soluble in water (1 mg/ml).
Sensitivity	Moisture sensitive.

Safety and Hazards(GHS)

Pictogram(s)	GHS08
Signal	Warning
Hazard Statements	H341:Suspected of causing genetic defects
Precautionary Statements	P405:Store locked up. P501:Dispose of contents/container to ... P281:Use personal protective equipment as required. P203:Obtain, read and follow all safety instructions before use. P318:if exposed or concerned, get medical advice.
WGK Germany	3
Reaxy-Rn	10510633

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Zhiwen Shen, Mingli Tian, Faxiang Wang, Yongle Liu, Jinhong Wu, Xianghong Li. (2024) Enhancing freeze-thaw stability and flavor in surimi products: Impact of virgin coconut oil and fish oil incorporation. Food Bioscience, 57 (103515). [PMID:] [10.1016/j.fbio.2023.103515]
2. Zhiwen Shen, Huaqian Gao, Wanqi Peng, Faxiang Wang, Yongle Liu, Jinhong Wu, Shaoyun Wang, Xianghong Li. (2023) Cryoprotective effect of soybean oil on surimi gels and the mechanism based on molecular dynamics simulation. JOURNAL OF TEXTURE STUDIES, (1): [PMID:37968073] [10.1111/jtxs.12812]
3. Xi Chen, Chenyao Tian, Hao Zhang, Hui Xie. (2023) Biodegradable Magnetic Hydrogel Robot with Multimodal Locomotion for Targeted Cargo Delivery. ACS Applied Materials & Interfaces, 15 (24): (28922–28932). [PMID:37283440] [10.1021/acsami.3c02703]
4. Hua-Jie Wang, Meng-Fei Hao, Guan Wang, Hao Peng, Fazli Wahid, Yan Yang, Lei Liang, Shan-Qin Liu, Ren-Long Li, Shu-Ying Feng. (2023) Zein nanospheres assisting inorganic and organic drug combination to overcome stent implantation-induced thrombosis and infection. SCIENCE OF THE TOTAL ENVIRONMENT, 873 (162438). [PMID:36842591] [10.1016/j.scitotenv.2023.162438]
5. Tianyi Chen, Haoze Chen, Anli Wang, Weixuan Yao, Zhongshi Xu, Binjie Wang, Jiye Wang, Yuanzhao Wu. (2023) Methyl Parathion Exposure Induces Development Toxicity and Cardiotoxicity in Zebrafish Embryos. Toxics, 11 (1): (84). [PMID:36668810] [10.3390/toxics11010084]
6. Yu Zhang, Dekun Cheng, Zhangtie Wang, Yiwen Guo, Ming Chang, Ruijie Liu. (2022) Protective Effect of Orbitides from Linseed (Linum usitatissimum L.) against Ultraviolet B-induced Photoaging in Zebrafish. PHOTOCHEMISTRY AND PHOTOBIOLOGY, [PMID:36484266] [10.1111/php.13758]
7. Xi Chen, Hao Zhang, Chenyao Tian, Jingzhi Jia, Hui Xie. (2022) Multiscale Magnetic Hydrogel Robot with a Core–Shell Structure for Active Targeted Delivery. ACS Applied Polymer Materials, 4 (11): (8645–8655). [PMID:] [10.1021/acsapm.2c01555]
8. Miaoqi Dai, Xiong Xiong, Anwei Cheng, Zhengtao Zhao, Qian Xiao. (2022) Development of pullulan-based nanocomposite films reinforced with starch nanocrystals for the preservation of fresh beef. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 103 (4): (1981-1993). [PMID:36260277] [10.1002/jsfa.12280]
9. Jiao Yang Lu, Xiaolong Zhou, Jialiang Yang, Yi Zhou, Binsheng He, Wei Tao Huang, Yajing Wang, Zhen Guo. (2022) Migration inhibition and selective cytotoxicity of cobalt hydroxide nanosheets on different cancer cell lines. NEW JOURNAL OF CHEMISTRY, 46 (21): (10289-10298). [PMID:] [10.1039/D2NJ01466A]
10. Qing Yu Liu, Zhen Qi Bu, Qing Feng Yao, Xuezhi Ding, Li Qiu Xia, Wei Tao Huang. (2021) Microwave-Assisted Synthesis of Chromium Oxide Nanoparticles for Fluorescence Biosensing of Mercury Ions and Molecular Logic Computing. ACS Applied Nano Materials, 4 (7): (7086–7096). [PMID:] [10.1021/acsanm.1c01102]
11. Zhenglu Wang, Shuiping Dai, Jinze Wang, Wei Du, Lin Zhu. (2021) Assessment on chronic and transgenerational toxicity of methamphetamine to Caenorhabditis elegans and associated aquatic risk through toxicity indicator sensitivity distribution (TISD) analysis. ENVIRONMENTAL POLLUTION, 288 (117696). [PMID:34243081] [10.1016/j.envpol.2021.117696]
12. Qi Liu, Yi Luo, Yun Zhao, Pingping Xiang, Jinyun Zhu, Wangwei Jing, Wenjing Jin, Mingyao Chen, Ruikang Tang, Hong Yu. (2022) Nano-hydroxyapatite accelerates vascular calcification via lysosome impairment and autophagy dysfunction in smooth muscle cells. Bioactive Materials, 8 (478). [PMID:34541414] [10.1016/j.bioactmat.2021.06.004]
13. Zhaohui Chen, Shuihua Wu. (2020) Protective Effects of SIRT6 Against Inflammation, Oxidative Stress, and Cell Apoptosis in Spinal Cord Injury. INFLAMMATION, 43 (5): (1751-1758). [PMID:32445068] [10.1007/s10753-020-01249-2]
14. Leipu Zhao, Cui Du, Chen Zhou, Shasha Sun, Yuanzheng Jia, Jiasheng Yuan, Guangming Song, Xin Zhou, Qian Zhao, Shengyang Yang. (2020) Structurally Ordered AgNPs@C3N4/GO Membranes toward Solar-Driven Freshwater Generation. ACS Sustainable Chemistry & Engineering, 8 (11): (4362–4370). [PMID:] [10.1021/acssuschemeng.9b06603]
15. Zeng Xin, Zhu Min, Liu Xiaohong, Chen Xuanmin, Yuan Yujia, Li Lan, Liu Jingping, Lu Yanrong, Cheng Jingqiu, Chen Younan. (2020) Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis. Nutrition & Metabolism, 17 (1): (1-14). [PMID:32021639] [10.1186/s12986-020-0434-8]
16. Zhigang Guo, Hu Guozhang, Hang Wang, Zhaohui Li, Naijie Liu. (2019) Ampelopsin inhibits human glioma through inducing apoptosis and autophagy dependent on ROS generation and JNK pathway. BIOMEDICINE & PHARMACOTHERAPY, 116 (108524). [PMID:31108349] [10.1016/j.biopha.2018.12.136]
17. Zhenqiang Wang, Liucan Wang, Neeraj Prabhakar, Yuxin Xing, Jessica M. Rosenholm, Jixi Zhang, Kaiyong Cai. (2019) CaP coated mesoporous polydopamine nanoparticles with responsive membrane permeation ability for combined photothermal and siRNA therapy. Acta Biomaterialia, 86 (416). [PMID:30611792] [10.1016/j.actbio.2019.01.002]
18. Nan Fang Nie, Xin Xing Zhang, Chu Shan Fang, Qiu Yan Zhu, Jiao Yang Lu, Fu Rui Zhang, Qing Feng Yao, Wei Tao Huang, Xue Zhi Ding, Li Qiu Xia. (2018) Game Theory in Molecular Nanosensing System for Rapid Detection of Hg2+ in Aqueous Solutions. Applied Sciences-Basel, 8 (12): (2530). [PMID:] [10.3390/app8122530]
19. Lin Xi, Yan Shu-Zhen, Qi Shan-Shan, Xu Qiao, Han Shuang-Shuang, Guo Ling-Yuan, Zhao Ning, Chen Shuang-Lin, Yu Shu-Qin. (2017) Transferrin-Modified Nanoparticles for Photodynamic Therapy Enhance the Antitumor Efficacy of Hypocrellin A. Frontiers in Pharmacology, 8 (815). [PMID:29209206] [10.3389/fphar.2017.00815]
20. Huan Wang, Ping Zhang, Jing Liu. (2017) Triethylene Tetramine Functionalized Magnetic Graphene Oxide Chitosan Composite with Superior Capacity for the Removal of Phosphate. JOURNAL OF CHEMICAL AND ENGINEERING DATA, 62 (10): (3341–3352). [PMID:] [10.1021/acs.jced.7b00417]
21. Huanan Wang, Wenqing Xu. (2017) Mito-methyl coumarin, a novel mitochondria-targeted drug with great antitumor potential was synthesized. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 489 (1). [PMID:28546001] [10.1016/j.bbrc.2017.05.116]
22. Lin Zhou, Yingzhen Lai, Wenxiu Huang, Sijia Huang, Zhiqiang Xu, Jiang Chen, Dong Wu. (2015) Biofunctionalization of microgroove titanium surfaces with an antimicrobial peptide to enhance their bactericidal activity and cytocompatibility. COLLOIDS AND SURFACES B-BIOINTERFACES, 128 (552). [PMID:25800357] [10.1016/j.colsurfb.2015.03.008]

References

1. Glass LJ et al.. (2017) Brain antibodies in the cortex and blood of people with schizophrenia and controls.. Transl Psychiatry, 7 (8): (e1192). [PMID:28786974]
2. Zhiwen Shen, Mingli Tian, Faxiang Wang, Yongle Liu, Jinhong Wu, Xianghong Li. (2024) Enhancing freeze-thaw stability and flavor in surimi products: Impact of virgin coconut oil and fish oil incorporation. Food Bioscience, 57 (103515). [PMID:] [10.1016/j.fbio.2023.103515]
3. Zhiwen Shen, Huaqian Gao, Wanqi Peng, Faxiang Wang, Yongle Liu, Jinhong Wu, Shaoyun Wang, Xianghong Li. (2023) Cryoprotective effect of soybean oil on surimi gels and the mechanism based on molecular dynamics simulation. JOURNAL OF TEXTURE STUDIES, (1): [PMID:37968073] [10.1111/jtxs.12812]
4. Xi Chen, Chenyao Tian, Hao Zhang, Hui Xie. (2023) Biodegradable Magnetic Hydrogel Robot with Multimodal Locomotion for Targeted Cargo Delivery. ACS Applied Materials & Interfaces, 15 (24): (28922–28932). [PMID:37283440] [10.1021/acsami.3c02703]
5. Hua-Jie Wang, Meng-Fei Hao, Guan Wang, Hao Peng, Fazli Wahid, Yan Yang, Lei Liang, Shan-Qin Liu, Ren-Long Li, Shu-Ying Feng. (2023) Zein nanospheres assisting inorganic and organic drug combination to overcome stent implantation-induced thrombosis and infection. SCIENCE OF THE TOTAL ENVIRONMENT, 873 (162438). [PMID:36842591] [10.1016/j.scitotenv.2023.162438]
6. Tianyi Chen, Haoze Chen, Anli Wang, Weixuan Yao, Zhongshi Xu, Binjie Wang, Jiye Wang, Yuanzhao Wu. (2023) Methyl Parathion Exposure Induces Development Toxicity and Cardiotoxicity in Zebrafish Embryos. Toxics, 11 (1): (84). [PMID:36668810] [10.3390/toxics11010084]
7. Yu Zhang, Dekun Cheng, Zhangtie Wang, Yiwen Guo, Ming Chang, Ruijie Liu. (2022) Protective Effect of Orbitides from Linseed (Linum usitatissimum L.) against Ultraviolet B-induced Photoaging in Zebrafish. PHOTOCHEMISTRY AND PHOTOBIOLOGY, [PMID:36484266] [10.1111/php.13758]
8. Xi Chen, Hao Zhang, Chenyao Tian, Jingzhi Jia, Hui Xie. (2022) Multiscale Magnetic Hydrogel Robot with a Core–Shell Structure for Active Targeted Delivery. ACS Applied Polymer Materials, 4 (11): (8645–8655). [PMID:] [10.1021/acsapm.2c01555]
9. Miaoqi Dai, Xiong Xiong, Anwei Cheng, Zhengtao Zhao, Qian Xiao. (2022) Development of pullulan-based nanocomposite films reinforced with starch nanocrystals for the preservation of fresh beef. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 103 (4): (1981-1993). [PMID:36260277] [10.1002/jsfa.12280]
10. Jiao Yang Lu, Xiaolong Zhou, Jialiang Yang, Yi Zhou, Binsheng He, Wei Tao Huang, Yajing Wang, Zhen Guo. (2022) Migration inhibition and selective cytotoxicity of cobalt hydroxide nanosheets on different cancer cell lines. NEW JOURNAL OF CHEMISTRY, 46 (21): (10289-10298). [PMID:] [10.1039/D2NJ01466A]
11. Qing Yu Liu, Zhen Qi Bu, Qing Feng Yao, Xuezhi Ding, Li Qiu Xia, Wei Tao Huang. (2021) Microwave-Assisted Synthesis of Chromium Oxide Nanoparticles for Fluorescence Biosensing of Mercury Ions and Molecular Logic Computing. ACS Applied Nano Materials, 4 (7): (7086–7096). [PMID:] [10.1021/acsanm.1c01102]
12. Zhenglu Wang, Shuiping Dai, Jinze Wang, Wei Du, Lin Zhu. (2021) Assessment on chronic and transgenerational toxicity of methamphetamine to Caenorhabditis elegans and associated aquatic risk through toxicity indicator sensitivity distribution (TISD) analysis. ENVIRONMENTAL POLLUTION, 288 (117696). [PMID:34243081] [10.1016/j.envpol.2021.117696]
13. Qi Liu, Yi Luo, Yun Zhao, Pingping Xiang, Jinyun Zhu, Wangwei Jing, Wenjing Jin, Mingyao Chen, Ruikang Tang, Hong Yu. (2022) Nano-hydroxyapatite accelerates vascular calcification via lysosome impairment and autophagy dysfunction in smooth muscle cells. Bioactive Materials, 8 (478). [PMID:34541414] [10.1016/j.bioactmat.2021.06.004]
14. Zhaohui Chen, Shuihua Wu. (2020) Protective Effects of SIRT6 Against Inflammation, Oxidative Stress, and Cell Apoptosis in Spinal Cord Injury. INFLAMMATION, 43 (5): (1751-1758). [PMID:32445068] [10.1007/s10753-020-01249-2]
15. Leipu Zhao, Cui Du, Chen Zhou, Shasha Sun, Yuanzheng Jia, Jiasheng Yuan, Guangming Song, Xin Zhou, Qian Zhao, Shengyang Yang. (2020) Structurally Ordered AgNPs@C3N4/GO Membranes toward Solar-Driven Freshwater Generation. ACS Sustainable Chemistry & Engineering, 8 (11): (4362–4370). [PMID:] [10.1021/acssuschemeng.9b06603]
16. Zeng Xin, Zhu Min, Liu Xiaohong, Chen Xuanmin, Yuan Yujia, Li Lan, Liu Jingping, Lu Yanrong, Cheng Jingqiu, Chen Younan. (2020) Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis. Nutrition & Metabolism, 17 (1): (1-14). [PMID:32021639] [10.1186/s12986-020-0434-8]
17. Zhigang Guo, Hu Guozhang, Hang Wang, Zhaohui Li, Naijie Liu. (2019) Ampelopsin inhibits human glioma through inducing apoptosis and autophagy dependent on ROS generation and JNK pathway. BIOMEDICINE & PHARMACOTHERAPY, 116 (108524). [PMID:31108349] [10.1016/j.biopha.2018.12.136]
18. Zhenqiang Wang, Liucan Wang, Neeraj Prabhakar, Yuxin Xing, Jessica M. Rosenholm, Jixi Zhang, Kaiyong Cai. (2019) CaP coated mesoporous polydopamine nanoparticles with responsive membrane permeation ability for combined photothermal and siRNA therapy. Acta Biomaterialia, 86 (416). [PMID:30611792] [10.1016/j.actbio.2019.01.002]
19. Nan Fang Nie, Xin Xing Zhang, Chu Shan Fang, Qiu Yan Zhu, Jiao Yang Lu, Fu Rui Zhang, Qing Feng Yao, Wei Tao Huang, Xue Zhi Ding, Li Qiu Xia. (2018) Game Theory in Molecular Nanosensing System for Rapid Detection of Hg2+ in Aqueous Solutions. Applied Sciences-Basel, 8 (12): (2530). [PMID:] [10.3390/app8122530]
20. Lin Xi, Yan Shu-Zhen, Qi Shan-Shan, Xu Qiao, Han Shuang-Shuang, Guo Ling-Yuan, Zhao Ning, Chen Shuang-Lin, Yu Shu-Qin. (2017) Transferrin-Modified Nanoparticles for Photodynamic Therapy Enhance the Antitumor Efficacy of Hypocrellin A. Frontiers in Pharmacology, 8 (815). [PMID:29209206] [10.3389/fphar.2017.00815]
21. Huan Wang, Ping Zhang, Jing Liu. (2017) Triethylene Tetramine Functionalized Magnetic Graphene Oxide Chitosan Composite with Superior Capacity for the Removal of Phosphate. JOURNAL OF CHEMICAL AND ENGINEERING DATA, 62 (10): (3341–3352). [PMID:] [10.1021/acs.jced.7b00417]
22. Huanan Wang, Wenqing Xu. (2017) Mito-methyl coumarin, a novel mitochondria-targeted drug with great antitumor potential was synthesized. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 489 (1). [PMID:28546001] [10.1016/j.bbrc.2017.05.116]
23. Lin Zhou, Yingzhen Lai, Wenxiu Huang, Sijia Huang, Zhiqiang Xu, Jiang Chen, Dong Wu. (2015) Biofunctionalization of microgroove titanium surfaces with an antimicrobial peptide to enhance their bactericidal activity and cytocompatibility. COLLOIDS AND SURFACES B-BIOINTERFACES, 128 (552). [PMID:25800357] [10.1016/j.colsurfb.2015.03.008]

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