Acridine Orange hydrochloride - >99%, high purity , CAS No.65-61-2

  • ≥99%
Item Number
A304298
Grouped product items
SKUSizeAvailabilityPrice Qty
A304298-25mg
25mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$121.90
A304298-100mg
100mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$340.90

Nucleic acid binding dye

Basic Description

SynonymsN3,N3,N6,N6-tetramethylacridine-3,6-diamine hydrochloride | acridine orange hydrochloride | Acridine Orange (Hydrochloride)
Specifications & Purity≥99%
Biochemical and Physiological MechanismsCell and organelle membrane permeable nucleic acid binding dye. Emits green fluorescence when bound to double-stranded DNA and red fluorescence when bound to RNA or single-stranded DNA. Used in cell cycle and apoptosis studies and as a lysosomal dye.
Storage TempProtected from light,Store at -20°C,Argon charged
Shipped InIce chest + Ice pads
Product Description

A rigorously purified zinc-free material suitable for use in flow cytometry.

Acridine Orange hydrochloride hydrate dye has been used:
• to stain intracellular acidic vesicles.
• to measure lysosomal membrane permeability (LMP). 
• or autophagy detection. Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Names and Identifiers

IUPAC Name 3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine;hydrochloride
INCHI InChI=1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
InChi Key VSTHNGLPHBTRMB-UHFFFAOYSA-N
Canonical SMILES CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C.Cl
Isomeric SMILES CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C.Cl
PubChem CID 517204
Molecular Weight 301.81

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

8 results found

Lot NumberCertificate TypeDateItem
K2429523Certificate of AnalysisNov 20, 2024 A304298
K2429524Certificate of AnalysisNov 20, 2024 A304298
K2429546Certificate of AnalysisNov 20, 2024 A304298
G2415813Certificate of AnalysisJul 05, 2024 A304298
G2416637Certificate of AnalysisJul 05, 2024 A304298
A2425086Certificate of AnalysisJun 14, 2022 A304298
G2205051Certificate of AnalysisJun 14, 2022 A304298
G2205052Certificate of AnalysisJun 14, 2022 A304298

Chemical and Physical Properties

SolubilitySolvent:water, Max Conc. mg/mL: 30.18, Max Conc. mM: 100; Solvent:DMSO, Max Conc. mg/mL: 30.18, Max Conc. mM: 100
SensitivityHygroscopic;light sensitive;heat sensitive

Safety and Hazards(GHS)

Pictogram(s) GHS08
Signal Danger
Hazard Statements

H340:May cause genetic defects

Precautionary Statements

P405:Store locked up.

P501:Dispose of contents/container to ...

P281:Use personal protective equipment as required.

P203:Obtain, read and follow all safety instructions before use.

P318:if exposed or concerned, get medical advice.

Related Documents

Citations of This Product

1. Hao-kun Hua, Hui-xing Guo, Wen-bin Liu, Zi-shang Liu, Chao-fan He, Wan-ting Du, Yang-yang Huang, Wei Xiong, Li-ren Ding, Guang-zhen Jiang.  (2023)  Dietary nicotinic acid promotes the growth and tryptophan metabolome of Chinese mitten crab (Eriocheir sinensis) through ACMSD and NADs.  Aquaculture Reports,  33  (15): (101800).  [PMID:10716937] [10.1016/j.aqrep.2023.101800]
2. Jun Fang Jiang, Juan Qiao, Xiao Yu Mu, Myeong Hee Moon, Li Qi.  (2017)  Fabrication of enzyme reactor utilizing magnetic porous polymer membrane for screening D-Amino acid oxidase inhibitors.  TALANTA,  165  (251).  [PMID:28153250] [10.1016/j.talanta.2016.12.055]
3. Xiaoyu Mu, Juan Qiao, Li Qi, Ying Liu, Huimin Ma.  (2014)  Construction of a d-Amino Acid Oxidase Reactor Based on Magnetic Nanoparticles Modified by a Reactive Polymer and Its Application in Screening Enzyme Inhibitors.  ACS Applied Materials & Interfaces,  (15): (12979–12987).  [PMID:24980686] [10.1021/am502901b]

References

1. Hao-kun Hua, Hui-xing Guo, Wen-bin Liu, Zi-shang Liu, Chao-fan He, Wan-ting Du, Yang-yang Huang, Wei Xiong, Li-ren Ding, Guang-zhen Jiang.  (2023)  Dietary nicotinic acid promotes the growth and tryptophan metabolome of Chinese mitten crab (Eriocheir sinensis) through ACMSD and NADs.  Aquaculture Reports,  33  (15): (101800).  [PMID:10716937] [10.1016/j.aqrep.2023.101800]
2. Jun Fang Jiang, Juan Qiao, Xiao Yu Mu, Myeong Hee Moon, Li Qi.  (2017)  Fabrication of enzyme reactor utilizing magnetic porous polymer membrane for screening D-Amino acid oxidase inhibitors.  TALANTA,  165  (251).  [PMID:28153250] [10.1016/j.talanta.2016.12.055]
3. Xiaoyu Mu, Juan Qiao, Li Qi, Ying Liu, Huimin Ma.  (2014)  Construction of a d-Amino Acid Oxidase Reactor Based on Magnetic Nanoparticles Modified by a Reactive Polymer and Its Application in Screening Enzyme Inhibitors.  ACS Applied Materials & Interfaces,  (15): (12979–12987).  [PMID:24980686] [10.1021/am502901b]

Solution Calculators