Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab176220-100μg | 100μg | In stock | $69.90 | |
| Ab176220-1mg | 1mg | In stock | $339.90 | |
| Ab176220-5mg | 5mg | In stock | $929.90 | |
| Ab176220-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,429.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | Adecatumumab (anti-EpCAM) - Primary antibody, specific to EPCAM, >95%, high purity, Human IgG1 |
|---|---|
| Synonyms | 17 1A antibody; 323/A3 antibody; Adenocarcinoma associated antigen antibody; Adenocarcinoma-associated antigen antibody; Antigen identified by monoclonal antibody AUA1 antibody; AUA1 antibody; CD326 antibody; CD326 antigen antibody; Cell surface glycoprot |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Specificity | EPCAM |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
| Action Type | INHIBITOR |
| Mechanism of action | INHIBITOR of Epithelial cell adhesion molecule inhibitor |
| Product Description | Adecatumumab (anti-EpCAM) is a full human monoclonal antibody of the IgG1 isotype, targeting human EpCAM. Adecatumumab (anti-EpCAM) is expressed in almost all adenocarcinomas, and its activity is not dependent of K-Ras status. |
| Isotype | Human IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 27.2 kDa (Light Chain) & 51.4 kDa (Heavy Chain), under reducing conditions; 180.0 kDa, under non-reducing conditions. |
| Purification Method | Protein A purified |
| Purity | >95% |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| CAS | 503605-66-1 |
Adecatumumab (anti-EpCAM) (Ab176220) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Adecatumumab (anti-EpCAM) (Ab176220). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Adecatumumab (anti-EpCAM) (Ab176220) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling EpCAM (red) with Adecatumumab (anti-EpCAM) (Ab176220). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Adecatumumab (anti-EpCAM) (Ab176220) - SEC
The purity of Adecatumumab (anti-EpCAM) (Ab176220) is more than 95% verified by HPLC.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404305 | Certificate of Analysis | Apr 10, 2024 | Ab176220 |
| ZJ24F0404307 | Certificate of Analysis | Apr 10, 2024 | Ab176220 |
| ZJ24F0404306 | Certificate of Analysis | Apr 10, 2024 | Ab176220 |
| 1. Simon, B B and 5 more authors.. (1990) Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2108441] |
| 2. Szala, S S and 6 more authors.. (1990) Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2333300] |
| 3. Strnad, J J and 9 more authors.. (1989) Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA.. Cancer research, (15): [PMID:2463074] |
| 4. Perez, M S MS and Walker, L E LE.. (1989) Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2469722] |
| 5. Linnenbach, A J AJ and 7 more authors.. (1989) Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2911574] |
| 6. Björk, P P and 8 more authors.. (1993) Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody.. The Journal of biological chemistry, (15): [PMID:7693697] |
| 7. Linnenbach, A J AJ and 7 more authors.. (1993) Retroposition in a family of carcinoma-associated antigen genes.. Molecular and cellular biology, [PMID:8382772] |
| 8. Chong, J M JM and Speicher, D W DW.. (2001) Determination of disulfide bond assignments and N-glycosylation sites of the human gastrointestinal carcinoma antigen GA733-2 (CO17-1A, EGP, KS1-4, KSA, and Ep-CAM).. The Journal of biological chemistry, (23): [PMID:11080501] |
| 9. Münz, Markus M and 5 more authors.. (2004) The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation.. Oncogene, (29): [PMID:15195135] |
| 10. Münz, Markus M, Zeidler, Reinhard R and Gires, Olivier O.. (2005) The tumour-associated antigen EpCAM upregulates the fatty acid binding protein E-FABP.. Cancer letters, (8): [PMID:15922867] |
| 11. Ladwein, Markus M and 8 more authors.. (2005) The cell-cell adhesion molecule EpCAM interacts directly with the tight junction protein claudin-7.. Experimental cell research, (1): [PMID:16054130] |
| 12. Munz, Markus M, Fellinger, Karin K, Hofmann, Tanja T, Schmitt, Barbel B and Gires, Olivier O.. (2008) Glycosylation is crucial for stability of tumour and cancer stem cell antigen EpCAM.. Frontiers in bioscience : a journal and virtual library, (1): [PMID:18508581] |
| 13. Sivagnanam, Mamata M and 15 more authors.. (2008) Identification of EpCAM as the gene for congenital tufting enteropathy.. Gastroenterology, [PMID:18572020] |
| 14. Ligtenberg, Marjolijn J L MJ and 17 more authors.. (2009) Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3' exons of TACSTD1.. Nature genetics, [PMID:19098912] |
| 15. Ng, Valerie Y VY, Ang, Sheu Ngo SN, Chan, Jia Xin JX and Choo, Andre B H AB.. (2010) Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells.. Stem cells (Dayton, Ohio), [PMID:19785009] |
| 16. Lu, Tung-Ying TY and 6 more authors.. (2010) Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells.. The Journal of biological chemistry, (19): [PMID:20064925] |
