mAb(8H1-B10-A12); Mouse anti Human AIF Antibody; WB, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab087780-10μl | 10μl | In stock | $79.90 | |
| Ab087780-50μl | 50μl | In stock | $229.90 | |
| Ab087780-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $329.90 | |
| Ab087780-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
mAb(8H1-B10-A12); Mouse anti Human AIF Antibody; WB, IF, ICC; Unconjugated
| Product Name | AIF Mouse mAb - Primary antibody, specific to AIFM1, Mouse IgG2a |
|---|---|
| Synonyms | AIF | AIFapoptosis-inducing factor 1, mitochondrial | AIFM1 | apoptosis-inducing factor, mitochondrion-associated, 1 | COXPD6 | MGC111425 | PDCD8 | PDCD8striatal apoptosis-inducing factor | programmed cell death 8 (apoptosis-inducing factor) | Programmed |
| Specifications & Purity | ExactAb™, Validated, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | AIFM1 |
| Immunogen | Recombinant protein of human AIF (AA 1-170) |
| Positive Control | WB: HeLa, Ramos, HepG2, MCF-7, Jurkat lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Mouse anti Human AIF Antibody, Monoclonal(8H1-B10-A12), could be used for WB, IF, ICC and so on . |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
AIF Mouse mAb (Ab087780) - Western Blot
All lanes: AIF Mouse mAb (Ab087780) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 66 kDa
Exposure time: 24.0 s
AIF Mouse mAb (Ab087780) - IF/ICC
Immunocytochemistry analysis of AIF in HeLa using AIF Mouse mAb (Ab087780).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303224 | Certificate of Analysis | Mar 14, 2024 | Ab087780 |
| ZJ24F0303225 | Certificate of Analysis | Mar 14, 2024 | Ab087780 |
| 1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
| 2. Susin, S A SA and 15 more authors.. (1999) Molecular characterization of mitochondrial apoptosis-inducing factor.. Nature, (4): [PMID:9989411] |
| 3. Daugas, E E and 6 more authors.. (2000) Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis.. FEBS letters, (7): [PMID:10913597] |
| 4. Ye, Hong H and 9 more authors.. (2002) DNA binding is required for the apoptogenic action of apoptosis inducing factor.. Nature structural biology, [PMID:12198487] |
| 5. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651] |
| 6. Otera, Hidenori H, Ohsakaya, Shigenori S, Nagaura, Zen-Ichiro Z, Ishihara, Naotada N and Mihara, Katsuyoshi K.. (2005) Export of mitochondrial AIF in response to proapoptotic stimuli depends on processing at the intermembrane space.. The EMBO journal, (6): [PMID:15775970] |
| 7. Delettre, Cécile C and 7 more authors.. (2006) AIFsh, a novel apoptosis-inducing factor (AIF) pro-apoptotic isoform with potential pathological relevance in human cancer.. The Journal of biological chemistry, (10): [PMID:16365034] |
| 8. Delettre, Cécile C and 5 more authors.. (2006) Identification and characterization of AIFsh2, a mitochondrial apoptosis-inducing factor (AIF) isoform with NADH oxidase activity.. The Journal of biological chemistry, (7): [PMID:16644725] |
| 9. Kim, Jong-Tae JT and 10 more authors.. (2006) Apoptosis-inducing factor (AIF) inhibits protein synthesis by interacting with the eukaryotic translation initiation factor 3 subunit p44 (eIF3g).. FEBS letters, (27): [PMID:17094969] |
| 10. Wilkinson, John C JC, Wilkinson, Amanda S AS, Galbán, Stefanie S, Csomos, Rebecca A RA and Duckett, Colin S CS.. (2008) Apoptosis-inducing factor is a target for ubiquitination through interaction with XIAP.. Molecular and cellular biology, [PMID:17967870] |
| 11. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 12. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 13. Son, Young-Ok YO and 5 more authors.. (2009) Apoptosis-inducing factor plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells.. Apoptosis : an international journal on programmed cell death, [PMID:19418225] |
| 14. Hangen, E E and 23 more authors.. (2010) A brain-specific isoform of mitochondrial apoptosis-inducing factor: AIF2.. Cell death and differentiation, [PMID:20111043] |
| 15. Ghezzi, Daniele D and 9 more authors.. (2010) Severe X-linked mitochondrial encephalomyopathy associated with a mutation in apoptosis-inducing factor.. American journal of human genetics, (9): [PMID:20362274] |
| 16. McKeller, M R MR and 15 more authors.. (2010) Vital function of PRELI and essential requirement of its LEA motif.. Cell death & disease, [PMID:21364629] |
| 17. Berger, Itai I and 9 more authors.. (2011) Early prenatal ventriculomegaly due to an AIFM1 mutation identified by linkage analysis and whole exome sequencing.. Molecular genetics and metabolism, [PMID:22019070] |
| 18. Lewis, Eric M EM, Wilkinson, Amanda S AS, Davis, Nicole Y NY, Horita, David A DA and Wilkinson, John C JC.. (2011) Nondegradative ubiquitination of apoptosis inducing factor (AIF) by X-linked inhibitor of apoptosis at a residue critical for AIF-mediated chromatin degradation.. Biochemistry, (27): [PMID:22103349] |
| 19. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 20. Rinaldi, Carlo C and 13 more authors.. (2012) Cowchock syndrome is associated with a mutation in apoptosis-inducing factor.. American journal of human genetics, (7): [PMID:23217327] |
| 21. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 22. Ferreira, Patricia P and 10 more authors.. (2014) Structural insights into the coenzyme mediated monomer-dimer transition of the pro-apoptotic apoptosis inducing factor.. Biochemistry, (1): [PMID:24914854] |
| 23. Kettwig, Matthias M and 8 more authors.. (2015) From ventriculomegaly to severe muscular atrophy: expansion of the clinical spectrum related to mutations in AIFM1.. Mitochondrion, [PMID:25583628] |
| 24. Ardissone, Anna A and 10 more authors.. (2015) A slowly progressive mitochondrial encephalomyopathy widens the spectrum of AIFM1 disorders.. Neurology, (26): [PMID:25934856] |
| 25. Zong, Liang L and 29 more authors.. (2015) Mutations in apoptosis-inducing factor cause X-linked recessive auditory neuropathy spectrum disorder.. Journal of medical genetics, [PMID:25986071] |
| 26. Hangen, Emilie E and 26 more authors.. (2015) Interaction between AIF and CHCHD4 Regulates Respiratory Chain Biogenesis.. Molecular cell, (18): [PMID:26004228] |
| 27. Diodato, Daria D and 15 more authors.. (2016) A novel AIFM1 mutation expands the phenotype to an infantile motor neuron disease.. European journal of human genetics : EJHG, [PMID:26173962] |
| 28. and Sevrioukova, Irina F IF.. (2016) Structure/Function Relations in AIFM1 Variants Associated with Neurodegenerative Disorders.. Journal of molecular biology, (11): [PMID:27178839] |
| 29. Brosey, Chris A CA and 9 more authors.. (2016) Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor.. Structure (London, England : 1993), (6): [PMID:27818101] |
| 30. Miyake, Noriko N and 28 more authors.. (2017) X-linked hypomyelination with spondylometaphyseal dysplasia (H-SMD) associated with mutations in AIFM1.. Neurogenetics, [PMID:28842795] |
