pAb; Rabbit anti Human AKT(phospho S473) Antibody; WB, IHC, IF/ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab087899-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $89.90 | |
| Ab087899-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $269.90 | |
| Ab087899-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $379.90 | |
| Ab087899-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
pAb; Rabbit anti Human AKT(phospho S473) Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | AKT(phospho S473) Antibody - Primary antibody, specific to AKT (phospho S473), Rabbit IgG |
|---|---|
| Synonyms | Akt(Phospho-Ser473) | AKT (phospho-S473) | AKT (phospho Ser473) | p-AKT (Ser473) | AKT 1 | AKT | AKT1 | AKT-1 | AKT1_HUMAN | C AKT | cAKT | MGC9965 | MGC99656 | Oncogene AKT1 | PKB | PKB alpha | PKB-ALPHA | PRKBA | Protein Kinase B Alpha | Protein kinase |
| Specifications & Purity | Validated, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | AKT (phospho S473) |
| Immunogen | A synthetic peptide derived from human AKT (phospho S473) (AA 431-480). |
| Positive Control | WB: HeLa, 3T3 lysates. IHC: Human breast carcinoma,Human lung, Human uterus, Mouse testis, Rat brain, Rat kidney, Rat spinal cord, Rat lung, Rat testis tissues. IF/ICC: Rat liver tissue. |
| Conjugation | Unconjugated |
| Grade | Validated |
| Product Description | Rabbit polyclonal to AKT (phospho S473), could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term. Store at -20°C for two years. Upon delivery aliquot. Avoid freeze/thaw cycle. |
AKT(phospho S473) Antibody (Ab087899) - Western Blot
All lanes: AKT(phospho S473) Antibody (Ab087899) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 57 kDa
Exposure time: 16.1 s
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunofluorescence analysis of rat-liver tissue.
1. AKT(phospho S473) Antibody (Ab087899) (red) was diluted at 1/200 (4°C, overnight).
2. Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
3. Picture B: DAPI (blue) 10 min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunofluorescence analysis of rat-liver tissue.
1. AKT(phospho S473) Antibody (Ab087899)(red) was diluted at 1/200 (4°C, overnight).
2. Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
3. Picture B: DAPI (blue) 10 min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AKT(phospho S473) Antibody (Ab087899). The picture on the right is blocked with the phospho peptide.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Human-lung tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IF/ICC | 1/50 - 1/200 |
| IHC | 1/100-1/300 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0707777 | Certificate of Analysis | Jul 04, 2024 | Ab087899 |
| ZJ24F0707776 | Certificate of Analysis | Jul 04, 2024 | Ab087899 |
