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Aladdin® 594 NHS Ester (Succinimidyl Ester) - ≥97%(HPLC), high purity , CAS No.295348-87-7

Item Number
A729269
Grouped product items
SKUSizeAvailabilityPrice Qty
A729269-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$929.90
A729269-5mg
5mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,699.90
View related series
Fluorescence dye (46)

Basic Description

Specifications & PurityEx:590nm, Em:618nm, mixture of isomers
Stability And StorageStore in freezer (-20 ℃) and protect from light.
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
GradeEm:618nm, Ex:590nm
Product Description

Aladdin® 594 is a bright red dye. Used for stable signal generation in imaging and flow cytometry, Aladdin® 594 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Aladdin® 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. 

The NHS ester (or succinimidyl ester) of Aladdin® 594 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Aladdin® conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores. 

Detailed information about this Aladdin® NHS ester:

Fluorophore label: Aladdin® 594 dye

Reactive group: NHS ester

Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides

Ex/Em of the conjugate: 590/617 nm

Extinction coefficient: 92,000 cm-1M-1

Molecular weight: 819.8

The Aladdin® NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO), and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.

AI Insight

Names and Identifiers

Molecular Weight 819.85(free base)

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
B2526242Certificate of AnalysisDec 03, 2024 A729269
B2526243Certificate of AnalysisDec 03, 2024 A729269
B2526244Certificate of AnalysisDec 03, 2024 A729269
B2526245Certificate of AnalysisDec 03, 2024 A729269

Chemical and Physical Properties

Solubilitysoluble in water, DMSO, DMF
SensitivityLight sensitive

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