As part of the LanthaScreen® TR-FRET toolbox of assay reagents, LanthaScreen® Amine Reactive Tb Chelate labeling reagent is available for assay development. The amine-reactive, isothiocyanate group conjugates to virtually any peptide or protein that contains one or more accessible amine moieties, such as the amine group found on a lysine residue or an unmodified N-terminal amine group of a peptide or protein. Amine-modified DNA oligonucleotides can be labeled as well. Energy transfer from the terbium donor to a suitable acceptor such as fluorescein is readily detected by monitoring an increase in acceptor fluorescence intensity. See the user guide for application-based protocols for labeling peptides containing free amino groups, amine-modified oligonucleotides, and IgG antibodies.
