BS(PEG)5 is a bis-succinimide ester-activated PEG compound used for crosslinking between primary amines (—NH2) in proteins and other molecules. The N-hydroxysuccinimide ester (NHS) groups at either end of the PEG5 spacer react specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds.
No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.
Features of BS(PEG)5:
• Reactive groups:sulfo-NHS ester (both ends) • Reactive towards: amino groups (primary amines) • NHS esters react with—NH2 groups at pH 7-9, forming stable amide bonds • Water-soluble; compare to non-pegylatedBS3 (also soluble) andDSS (water-insoluble) • Membrane-impermeable, allowing for cell surface labeling • Irreversibly crosslink proteins or peptides by flexible PEG spacer arms • Polyethylene glycol spacer arms help maintain conjugate solubility • Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects • PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity • Ideal for small molecule or peptide conjugations
