Basic Description

Specifications & Purity	≥99%
Storage Temp	Store at -20°C,Argon charged,Desiccated
Shipped In	Ice chest + Ice pads
Product Description	Product introduction： The N-hydroxysuccinimide ester (NHS)-biotin modification helps in the biotinylation of the target lysine residue at its ε-amino group and identifies the surface lysine residues. It is used as a tool to identify the interaction of proteins with several biological molecules, like protein-polysaccharide interactions, protein-protein interactions, protein-nucleic acid interactions, and protein-ligand interactions. NHS-biotin may be used in the biotinylation of proteins and peptides. It is typically coupled to primary amine in the pH range of 6.5-8.5.

Names and Identifiers

IUPAC Name	(2,5-dioxopyrrolidin-1-yl) 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate
INCHI	InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
InChi Key	YMXHPSHLTSZXKH-RVBZMBCESA-N
Canonical SMILES	C1CC(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3
Isomeric SMILES	C1CC(=O)N(C1=O)OC(=O)CCCC[C@H]2[C@@H]3[C@H](CS2)NC(=O)N3
WGK Germany	3
PubChem CID	6710714
Molecular Weight	341.38
Beilstein	4720781
Reaxy-Rn	4720781

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
F2426271	Certificate of Analysis	Jul 04, 2024	A638743
F2417102	Certificate of Analysis	Jun 25, 2024	A638743

Chemical and Physical Properties

Solubility	DMF(Dimethylformamide),DMSO(Dimethylsulfoxide)
Sensitivity	Moisture sensitive；air sensitive；heat sensitive
Melt Point(°C)	140°C

Safety and Hazards(GHS)

Pictogram(s)	GHS07
Signal	Warning
Hazard Statements	H315:Causes skin irritation H319:Causes serious eye irritation H335:May cause respiratory irritation
Precautionary Statements	P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of water. P321:Specific treatment (see ... on this label). P405:Store locked up. P501:Dispose of contents/container to ... P264:Wash hands [and …] thoroughly after handling. P271:Use only outdoors or in a well-ventilated area. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P403+P233:Store in a well-ventilated place. Keep container tightly closed. P362+P364:Take off contaminated clothing and wash it before reuse. P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes. P337+P317:If eye irritation persists: Get medical help. P332+P317:If skin irritation occurs: Get medical help. P319:Get medical help if you feel unwell.
WGK Germany	3
Reaxy-Rn	4720781

References

1. Wang JJ, Liu F, Yang F, Wang YZ, Qi X, Li Y, Hu Q, Zhu MX, Xu TL. (2020) Disruption of auto-inhibition underlies conformational signaling of ASIC1a to induce neuronal necroptosis.. Nat Commun, 11 (1): (475). [PMID:31980622]
2. Koleva L, Bovt E, Ataullakhanov F, Sinauridze E. () Erythrocytes as Carriers: From Drug Delivery to Biosensors.. Pharmaceutics, [PMID:32197542]
3. Pak JS, DeLoughery ZJ, Wang J, Acharya N, Park Y, Jaworski A, Özkan E. () NELL2-Robo3 complex structure reveals mechanisms of receptor activation for axon guidance.. Nat Commun, [PMID:32198364]
4. Zeng W, Niu L, Wang Z, Wang X, Wang Y, Pan L, Lu Z, Cui G, Weng W, Wang M, Meng X, Wang Z. () Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model.. Hortic Res, [PMID:32194967]
5. Tharp JM, Hampton JT, Reed CA, Ehnbom A, Chen PC, Morse JS, Kurra Y, Pérez LM, Xu S, Liu WR. () An amber obligate active site-directed ligand evolution technique for phage display.. Nat Commun, [PMID:32170178]
6. Zhang C, He T, Vedadghavami A, Bajpayee AG. () Avidin-biotin technology to synthesize multi-arm nano-construct for drug delivery.. MethodsX, [PMID:32405463]
7. Ohlendorf R, Wisniowska A, Desai M, Barandov A, Slusarczyk AL, Li N, Jasanoff A. () Target-responsive vasoactive probes for ultrasensitive molecular imaging.. Nat Commun, [PMID:32404879]
8. Seo JA, Kang MC, Yang WM, Hwang WM, Kim SS, Hong SH, Heo JI, Vijyakumar A, Pereira de Moura L, Uner A, Huang H, Lee SH, Lima IS, Park KS, Kim MS, Dagon Y, Willnow TE, Aroda V, Ciaraldi TP, Henry RR, Kim YB. () Apolipoprotein\xa0J is a hepatokine regulating muscle glucose metabolism and insulin sensitivity.. Nat Commun, [PMID:32332780]
9. Wymant JM, Sayers EJ, Muir D, Jones AT. () Strategic Trastuzumab Mediated Crosslinking Driving Concomitant HER2 and HER3 Endocytosis and Degradation in Breast Cancer.. J Cancer, [PMID:32231734]
10. Rao L, Hülsemann M, Gennerich A. () Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein.. Methods Mol Biol, [PMID:28940064]
11. Cardote TAF, Gadd MS, Ciulli A. () Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase Complex.. Structure, [PMID:28591624]
12. Brouillette RB, Phillips EK, Ayithan N, Maury W. () Differences in Glycoprotein Complex Receptor Binding Site Accessibility Prompt Poor Cross-Reactivity of Neutralizing Antibodies between Closely Related Arenaviruses.. J Virol, [PMID:28100617]
13. Wolhuter K, Whitwell HJ, Switzer CH, Burgoyne JR, Timms JF, Eaton P. () Evidence against Stable Protein S-Nitrosylation as a Widespread Mechanism of Post-translational Regulation.. Mol Cell, [PMID:29358077]
14. Pazina T, James AM, MacFarlane AW, Bezman NA, Henning KA, Bee C, Graziano RF, Robbins MD, Cohen AD, Campbell KS. () The anti-SLAMF7 antibody elotuzumab mediates NK cell activation through both CD16-dependent and -independent mechanisms.. Oncoimmunology, [PMID:28932638]
15. Diner I,Hales CM,Bishof I,Rabenold L,Duong DM,Yi H,Laur O,Gearing M,Troncoso J,Thambisetty M,Lah JJ,Levey AI,Seyfried NT. () Aggregation properties of the small nuclear ribonucleoprotein U1-70K in Alzheimer disease.. , [PMID:25355317]
16. Lei L, Yang YR, Tran K, Wang Y, Chiang CI, Ozorowski G, Xiao Y, Ward AB, Wyatt RT, Li Y. () The HIV-1 Envelope Glycoprotein C3/V4 Region Defines a Prevalent Neutralization Epitope following Immunization.. Cell Rep, [PMID:30970260]

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SKU	Size	Availability	Price
A638743-25mg	25mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
A638743-50mg	50mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$109.90
A638743-100mg	100mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$189.90

Aladdin™ NHS-Biotin - ≥99%, high purity , CAS No.35013-72-0