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Basic Description
| Storage Temp | Store at 2-8°C |
|---|---|
| Shipped In | Wet ice |
| Product Description | Product introduction： The Click-iT™ O-GlcNAc Enzymatic Labeling System provides a highly sensitive and efficient method for the in-vitro modification of O-GlcNAc modified proteins. Proteins are enzymatically labeled utilizing the permissive mutant β-1,4-galactosyltransferase (Gal-T1 (Y289L)) which transfers azido-modified galactose (GalNAz) from UDP-GalNAz to O-GlcNAc residues. Then, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT™ Glycoprotein Detection kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne). Labeling and detection can be completed in less than 24 hours and the Click-iT™ glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of this important post-translational modification. • Green Features:Less hazardous • Labeling Target:Proteins(Glycoproteins) |
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Related Documents
References
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| 2. Li X, Molina H, Huang H, Zhang YY, Liu M, Qian SW, Slawson C, Dias WB, Pandey A, Hart GW, Lane MD, Tang QQ,. () O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation.. J Biol Chem, [PMID:19478079] |
| 3. Clark PM, Dweck JF, Mason DE, Hart CR, Buck SB, Peters EC, Agnew BJ, Hsieh-Wilson LC,. () Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.. J Am Chem Soc, [PMID:18683930] |
| 4. Yadav V, Paniliatis BJ, Shi H, Lee K, Cebe P, Kaplan DL,. () Novel in vivo-degradable cellulose-chitin copolymer from metabolically engineered Gluconacetobacter xylinus.. Appl Environ Microbiol, [PMID:20656868] |
| 5. Wang Z, Pandey A, Hart GW. () Dynamic interplay between O-linked N-acetylglucosaminylation and glycogen synthase kinase-3-dependent phosphorylation.. Mol Cell Proteomics, [PMID:17507370] |
| 6. Dehennaut V, Slomianny MC, Page A, Vercoutter-Edouart AS, Jessus C, Michalski JC, Vilain JP, Bodart JF, Lefebvre T,. () Identification of structural and functional O-linked N-acetylglucosamine-bearing proteins in Xenopus laevis oocyte.. Mol Cell Proteomics, [PMID:18617508] |
| 7. Murrey HE, Hsieh-Wilson LC,. () The chemical neurobiology of carbohydrates.. Chem Rev, [PMID:18452339] |