Alanine is a nonessential amino acid that plays a key role in the glucose-alanine cycle between muscle tissue and the liver. In amino acid-degrading tissues such as muscle, amino groups are pooled as glutamate by transamination reactions. The amino group of glutamate is transferred to pyruvate via alanine aminotransferase, forming alanine and α-ketoglutarate. The alanine is passed into the blood and transported to the liver. This reaction is reversed in the liver where pyruvate can be used in gluconeogenesis to form glucose, which may return to other tissues through the circulatory system. Increased alanine levels correlate with higher blood pressure, energy intake, cholesterol levels, and body mass index.Alanine Assay Kit has been used to determine the intracellular alanine pool.Suitability: Suitable for alanine detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.Principle: In this kit, alanine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex= 535/λem= 587 nm) product, proportional to the alanine present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).
1.Deanna N Edwards,Verra M Ngwa,Ariel L Raybuck,Shan Wang,Yoonha Hwang,Laura C Kim,Sung Hoon Cho,Yeeun Paik,Qingfei Wang,Siyuan Zhang,H Charles Manning,Jeffrey C Rathmell,Rebecca S Cook,Mark R Boothby,Jin Chen. (2020-12-16) Selective glutamine metabolism inhibition in tumor cells improves antitumor T lymphocyte activity in triple-negative breast cancer.. The Journal of clinical investigation, 131 ((4)):[PMID:33320840]
2.Dmitry Frank,Ruslan Kuts,Philip Tsenter,Benjamin F Gruenbaum,Yulia Grinshpun,Vladislav Zvenigorodsky,Ilan Shelef,Dmitry Natanel,Evgeny Brotfain,Alexander Zlotnik,Matthew Boyko. (2019-06-04) The effect of pyruvate on the development and progression of post-stroke depression: A new therapeutic approach.. Neuropharmacology, 155 (173-184). [PMID:31153808]
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