Alexa Fluor™ 700 NHS Ester (Succinimidyl Ester) , CAS No.A638844

  • Molecular Weight:  ∼1400
Item Number
A638844
Grouped product items
SKUSizeAvailabilityPrice Qty
A638844-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$969.90
A638844-5mg
5mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,759.90

Basic Description

SynonymsAlexa Fluor 700 equivalent
Specifications & PurityEx:696nm, Em:719nm
Stability And StorageStore in freezer (-5 to -30 ℃) and protect from light.
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
GradeEm:719nm, Ex:696nm
Product Description

AF 700 is a bright and photostable near-IR dye that can be excited with a xenon-arc lamp, far-red diode lasers, or dye pumped lasers operating in the 675–700 nm range. Used for stable signal generation in imaging and flow cytometry, AF 700 dye is water soluble and pH-insensitive from pH 4 to pH 10. Fluorescence of this long-wavelength AF dye is not visible to the human eye but is readily detected by most imaging systems. In addition to reactive dye formulations, we offer AF 700 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.The NHS ester (or succinimidyl ester) of AF 700 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Alexa Fluor™ conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Fluorophore label: AF 700 dye
Reactive group: NHS ester
Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides
Ex/Em of the conjugate: 702/723 nm
Extinction coefficient: 205,000 cm-1M-1
Molecular weight: ∼1400

Product Properties

ReactivityAmine

Names and Identifiers

Molecular Weight ∼1400

Certificates

Certificate of Analysis(COA)

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Chemical and Physical Properties

SensitivityLight sensitive

Related Documents

References

1. Krutzik PO, Nolan GP.  ()  Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling..  Nat Methods,      [PMID:16628206] [http://pubmed.ncbi.nlm.nih.gov/16628206]
2. Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugland RP, Haugland RP.  ()  Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates..  J Histochem Cytochem,      [PMID:14623938] [http://pubmed.ncbi.nlm.nih.gov/14623938]
3. Choi HM, Chang JY, Trinh le A, Padilla JE, Fraser SE, Pierce NA,.  ()  Programmable in situ amplification for multiplexed imaging of mRNA expression..  Nat Biotechnol,      [PMID:21037591] [http://pubmed.ncbi.nlm.nih.gov/21037591]
4. Clutter MR, Heffner GC, Krutzik PO, Sachen KL, Nolan GP,.  ()  Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry..  Cytometry A,      [PMID:20824632] [http://pubmed.ncbi.nlm.nih.gov/20824632]
5. Appelblom H, Nurmi J, Soukka T, Pasternack M, Penttilä KE, Lövgren T, Niemelä P,.  ()  Homogeneous TR-FRET high-throughput screening assay for calcium-dependent multimerization of sorcin..  J Biomol Screen,      [PMID:17579123] [http://pubmed.ncbi.nlm.nih.gov/17579123]

Solution Calculators