Alpha-lytic protease

  • ≥0.0005 U/mg
Item Number
A755283
Grouped product items
SKUSizeAvailabilityPrice Qty
A755283-20μg
20μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$131.90
A755283-5×20μg
5×20μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$495.90

Basic Description

Specifications & Purity≥0.0005 U/mg
Storage TempStore at -80°C
Shipped InIce chest + Ice pads
Product DescriptionAlpha-lytic protease (aLP) is an alternative specificity protease for proteomics applications. This protease cleaves after T, A, S, and V residues. It generates peptides of similar average length as trypsin.

aLP was first isolated from the myxobacterium Lysobacter enzymogenes. The pro-form of aLP is 397 amino acids long. In its mature form, aLP is 198 amino acids long. Its tertiary structural core resembles those of pancreatic serine proteases.

Crystal structure studies of aLP have been reported. Several studies are available on the active site and catalytic mechanism of aLP. The role of the pro-region in the activation, secretion and folding of aLP has been studied.

The activity of aLP in the presence of various solution components is as follows:

  • 0.1% sodium deoxycholate: ~1.75-fold enhanced activity
  • 1.0% sodium deoxycholate: ~60% activity
  • 0.1% SDS: ~50% activity
  • 1.0% SDS: ~40% activity
  • 1 M guanidine HCl: ~20% activity
  • 4 M guanidine HCl: ~1% activity (essentially inactivated)

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Related Documents

Solution Calculators