Grouped product items

SKU

Size

Availability

Price

Qty

A491865-1mg

1mg

Available within 8-12 weeks(?)

$59.90

A491865-5mg

5mg

Available within 4-8 weeks(?)

$189.90

A491865-25mg

25mg

Available within 4-8 weeks(?)

$629.90

Basic Description

Synonyms	ADHP \| J-100008 \| Amplex red \| A 6550 \| N13275 \| BCP29392 \| HY-101880 \| 1-(3,7-dihydroxy-10H-phenoxazin-10-yl)ethan-1-one \| Ethanone, 1-(3,7-dihydroxy-10H-phenoxazin-10-yl)- \| Amplisyn Red;ADHP;Amplex red reagent;1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)etha
Specifications & Purity	≥96%
Storage Temp	Store at 2-8°C,Argon charged
Shipped In	Wet ice
Product Description	Product Describtion: Fluorogenic substrate for peroxidase;Ampliflu Red is used for the following applications: Quantification of inflammatory mediators;Determination of free and esterified cholesterol contents; Evaluation of compounds as substrates for GABA-AT compounds. We are pleased to introduce our serieal Amplite™ fluorogenic substrates to address this unmet need. Our Amplite™ ADHP is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc. Although ADHP exhibits very high sensitivity for detecting peroxidate activity either in a fluorimetric or colorimetric mode, it requires higher pH to reach its maximum fluorescence. We are pleased to introduce our Amplite™ ADAP that has better water solubility and stronger fluorescence than ADHP. Addtionally, our Amplite™ is the only fluorogenic peroxidase substrate that generates fluorescent precipitates upon the peroxidase-catalyzed oxidation.

Names and Identifiers

IUPAC Name	1-(3,7-dihydroxyphenoxazin-10-yl)ethanone
INCHI	InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
InChi Key	PKYCWFICOKSIHZ-UHFFFAOYSA-N
Canonical SMILES	CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O
Isomeric SMILES	CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O
PubChem CID	167453
Molecular Weight	257.241

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

9 results found

Lot Number	Certificate Type	Date	Item
F2425084	Certificate of Analysis	Jul 01, 2024	A491865
F2425085	Certificate of Analysis	Jul 01, 2024	A491865
F2412080	Certificate of Analysis	Jun 17, 2024	A491865
F2411041	Certificate of Analysis	Jun 14, 2024	A491865
B2419173	Certificate of Analysis	Dec 20, 2022	A491865
C2411064	Certificate of Analysis	Dec 20, 2022	A491865
L2210099	Certificate of Analysis	Dec 20, 2022	A491865
L2210100	Certificate of Analysis	Dec 20, 2022	A491865
L2210110	Certificate of Analysis	Dec 20, 2022	A491865

Chemical and Physical Properties

Sensitivity	Light & Air Sensitive.
Melt Point(°C)	> 250 °C

Safety and Hazards(GHS)

Pictogram(s)	GHS07
Signal	Warning
Hazard Statements	H302:Harmful if swallowed
Precautionary Statements	P501:Dispose of contents/container to ... P264:Wash hands [and …] thoroughly after handling. P270:Do not eat, drink or smoke when using this product. P330:Rinse mouth. P301+P317:IF SWALLOWED: Get medical help.

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Hongwei Su, Xin Chen, Hua Hu, Xiaofang Zhang, Guilong Yan, Zhenyu Li, Li Wang, Dong Xiang, Chunxia Zhao, Yuanpeng Wu, Yong Du, Jingyu Chen. (2024) Carbon/MnO2-x nanofibrous membrane for superior O/W emulsions purification with robust and comprehensive-fouling mitigation. Surfaces and Interfaces, 44 (30): (103660). [PMID:27502274] [10.1016/j.surfin.2023.103660]
2. Qian Yang, Heng Qian, Yang Guo, Xueling Bai, Jing Li, Chuncheng Chen. (2023) Rapid Release of Halocarbons from Saline Water by Iron-Based Photochemistry. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 57 (49): (20781–20791). [PMID:38010203] [10.1021/acs.est.3c05552]
3. Qinqin Jiang, Bin Qiao, Xiaohong Lin, Jin Cao, Nan Zhang, Huanling Guo, Weiwei Liu, Lingyu Zhu, Xue Xie, Li Wan, Rui Tang, Bing Liang, Dong Wang, Zhigang Wang, Yang Zhou, HaiTao Ran, Pan Li. (2022) A hydrogen peroxide economizer for on-demand oxygen production-assisted robust sonodynamic immunotherapy. Theranostics, 12 (1): ( 59–75). [PMID:34987634] [10.7150/thno.64862]
4. Chan Song, Haibo Liu, Linlin Zhang, Jie Wang, Chenglong Zhao, Qinyuan Xu, Cheng Yao. (2022) FeS nanoparticles embedded in 2D carbon nanosheets as novel nanozymes with peroxidase-like activity for colorimetric and fluorescence assay of H2O2 and antioxidant capacity. SENSORS AND ACTUATORS B-CHEMICAL, 353 (131131). [PMID:] [10.1016/j.snb.2021.131131]
5. Nuanfei Zhu, Xuesong Li, Ye Liu, Jingfu Liu, Yawei Wang, Xiangyang Wu, Zhen Zhang. (2021) Dual amplified ratiometric fluorescence ELISA based on G-quadruplex/hemin DNAzyme using tetrahedral DNA nanostructure as scaffold for ultrasensitive detection of dibutyl phthalate in aquatic system. SCIENCE OF THE TOTAL ENVIRONMENT, 784 (147212). [PMID:33905933] [10.1016/j.scitotenv.2021.147212]
6. Tian Guan, Dayu Liu, Xiangmei Li, Bowen Shu, Mengting Li, Yingju Liu, Zhenlin Xu, Yudong Shen, Yuanming Sun, Hongtao Lei, Xing Shen. (2020) Magnet-actuated droplet microfluidic immunosensor coupled with gel imager for detection of microcystin-LR in aquatic products. TALANTA, 219 (121329). [PMID:32887063] [10.1016/j.talanta.2020.121329]
7. Tiantian Yao, Anran Liu, Yong Liu, Min Wei, Wei Wei, Songqin Liu. (2019) Ratiometric fluorescence sensor for organophosphorus pesticide detection based on opposite responses of two fluorescence reagents to MnO2 nanosheets. BIOSENSORS & BIOELECTRONICS, 145 (111705). [PMID:31550630] [10.1016/j.bios.2019.111705]
8. Daoqing Fan, Changshuai Shang, Wenling Gu, Erkang Wang, Shaojun Dong. (2017) Introducing Ratiometric Fluorescence to MnO2 Nanosheet-Based Biosensing: A Simple, Label-Free Ratiometric Fluorescent Sensor Programmed by Cascade Logic Circuit for Ultrasensitive GSH Detection. ACS Applied Materials & Interfaces, 9 (31): (25870–25877). [PMID:28696093] [10.1021/acsami.7b07369]

References

1. Hongwei Su, Xin Chen, Hua Hu, Xiaofang Zhang, Guilong Yan, Zhenyu Li, Li Wang, Dong Xiang, Chunxia Zhao, Yuanpeng Wu, Yong Du, Jingyu Chen. (2024) Carbon/MnO2-x nanofibrous membrane for superior O/W emulsions purification with robust and comprehensive-fouling mitigation. Surfaces and Interfaces, 44 (30): (103660). [PMID:27502274] [10.1016/j.surfin.2023.103660]
2. Qian Yang, Heng Qian, Yang Guo, Xueling Bai, Jing Li, Chuncheng Chen. (2023) Rapid Release of Halocarbons from Saline Water by Iron-Based Photochemistry. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 57 (49): (20781–20791). [PMID:38010203] [10.1021/acs.est.3c05552]
3. Qinqin Jiang, Bin Qiao, Xiaohong Lin, Jin Cao, Nan Zhang, Huanling Guo, Weiwei Liu, Lingyu Zhu, Xue Xie, Li Wan, Rui Tang, Bing Liang, Dong Wang, Zhigang Wang, Yang Zhou, HaiTao Ran, Pan Li. (2022) A hydrogen peroxide economizer for on-demand oxygen production-assisted robust sonodynamic immunotherapy. Theranostics, 12 (1): ( 59–75). [PMID:34987634] [10.7150/thno.64862]
4. Chan Song, Haibo Liu, Linlin Zhang, Jie Wang, Chenglong Zhao, Qinyuan Xu, Cheng Yao. (2022) FeS nanoparticles embedded in 2D carbon nanosheets as novel nanozymes with peroxidase-like activity for colorimetric and fluorescence assay of H2O2 and antioxidant capacity. SENSORS AND ACTUATORS B-CHEMICAL, 353 (131131). [PMID:] [10.1016/j.snb.2021.131131]
5. Nuanfei Zhu, Xuesong Li, Ye Liu, Jingfu Liu, Yawei Wang, Xiangyang Wu, Zhen Zhang. (2021) Dual amplified ratiometric fluorescence ELISA based on G-quadruplex/hemin DNAzyme using tetrahedral DNA nanostructure as scaffold for ultrasensitive detection of dibutyl phthalate in aquatic system. SCIENCE OF THE TOTAL ENVIRONMENT, 784 (147212). [PMID:33905933] [10.1016/j.scitotenv.2021.147212]
6. Tian Guan, Dayu Liu, Xiangmei Li, Bowen Shu, Mengting Li, Yingju Liu, Zhenlin Xu, Yudong Shen, Yuanming Sun, Hongtao Lei, Xing Shen. (2020) Magnet-actuated droplet microfluidic immunosensor coupled with gel imager for detection of microcystin-LR in aquatic products. TALANTA, 219 (121329). [PMID:32887063] [10.1016/j.talanta.2020.121329]
7. Tiantian Yao, Anran Liu, Yong Liu, Min Wei, Wei Wei, Songqin Liu. (2019) Ratiometric fluorescence sensor for organophosphorus pesticide detection based on opposite responses of two fluorescence reagents to MnO2 nanosheets. BIOSENSORS & BIOELECTRONICS, 145 (111705). [PMID:31550630] [10.1016/j.bios.2019.111705]
8. Daoqing Fan, Changshuai Shang, Wenling Gu, Erkang Wang, Shaojun Dong. (2017) Introducing Ratiometric Fluorescence to MnO2 Nanosheet-Based Biosensing: A Simple, Label-Free Ratiometric Fluorescent Sensor Programmed by Cascade Logic Circuit for Ultrasensitive GSH Detection. ACS Applied Materials & Interfaces, 9 (31): (25870–25877). [PMID:28696093] [10.1021/acsami.7b07369]

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Ampliflu Red - 96%, high purity , CAS No.119171-73-2