Antonia Red-lysine-dextran

Item Number

A491542

• Synonyms: Antonia Red™-lysine-dextran
• Storage Temp: Room temperature,Argon charged
• Shipped In: Normal

Product Description

mesenteroides labelled with Lysine. Lysine dextran is then functionalized with Antonia Red under mild

conditions to form Antonia Red Lysine Dextran. After purification, the products are controlled for Mw,

appearance, solubility, DS, pH, free Lysine and free dye. The products are designated by the

approximate molecular weights of the dextran fractions used. Thus, for example, the product Antonia

Red Lysine Dextran 4 has a molecular weight of approx. 4000 Da. The actual molecular weight is

determined by GPC. This value is supplied with the Certificate of Analysis. The dextran is essentially a linear α-(1-6)-linked glucose chain with

however a low percentage (2-5%) of α-(1-3) branches distributed along the chain. The dextran

fractions used are from Mw of 4000 to 500000 and are carefully controlled by GPC, optical rotation,

absorbance and other control parameters.Physical propertiesAntonia Red is a novel red-shifted dye similar to Texas Red and is developed and produced by Aladdin

Consultancy AB. Antonia Red Lysine Dextrans are purple powders that are readily soluble in water or

electrolyte solutions. Antonia Red Lysine Dextran are insoluble in most organic solvents, such as

ethanol, methanol, acetone, chloroform, ethyl acetate etc. The degree of substitution, (DS) is between

0.01-0.02 (mol Lysine/mol Glucose) and 0.001-0.01 (mol Antonia Red/mol Glucose).Spectral data Antonia Red-lysine-dextran has an absorbance maximum at 583nm and an emission wavelength of 602 nm in borate buffer at pH 9.0.Stability

No prospective stability studies on Antonia Red Lysine Dextrans have been performed yet. However,

the structural properties of the dextran and of the carbamide linkage of the Lysine to the dextran

chain as well as the convalent bond between Lysine and Antonia Red would suggest high stability of

the product. It is recommended that the products are stored in air-tight containers. Antonia Red

Lysine Dextrans may be stored at ambient temperatures. Application

Antonia Red-lysine-dextrans offer easy conjugation for biomolecules and may be used for preserving cells in a” life like” state. Antonia Red shows bright fluorescence, has good photo- and chemical stability and is non-pH dependent.Dextrans carrying amino groups are valuable to the scientific community as versatile tools for bioconjugation and fixation in living systems1. For conjugation, the free amino group of the Lysine structure will be able to covalently bind to activated entities such as NHS-esters or isothiocyanates or be coupled to carboxylic acids using common peptide coupling reagents such as DCC, HOBt and or HATU. Cell and tissue fixation is performed in order to preserve components in a “life-like state” and to make cells permeable to allow antibodies to access cellular structures, for example for microscopy studies or immunostaining. The amino groups on Lysine Dextran reacts with the fixing agent (here: formaldehyde or glutaraldehyde) to form a covalent crosslinking with biomolecules such as proteins and lipids, immobilizing the whole system and the dextran. This is of particular importance when evaluating biological events qualitatively or quantitatively in molecular imaging. Without fixation, these structures within a living system would fall apart and diffuse rapidly. Antonia Red is a fluorescent dye that has an excitation maximum at 583 nm and an emission maximum at 611 nm, making it an analogue of the popular Texas Red dye.

Product specifications and parameters：

SKU	NAME	Molecular Weight
A491542-4kDa	Antonia Red-lysine-dextran	4kDa
A491542-20kDa	Antonia Red-lysine-dextran	20kDa
A491542-40kDa	Antonia Red-lysine-dextran	40kDa
A491542-70kDa	Antonia Red-lysine-dextran	70kDa
A491542-150kDa	Antonia Red-lysine-dextran	150kDa