ATP6V1D Antibody - Primary antibody, specific to ATP6V1D, Rabbit IgG
Synonyms
ATP6M antibody; ATP6V1D antibody; V-ATPase 28 kDa accessory proteinantibody; V-ATPase subunit D antibody; V-type proton ATPase subunit D antibody; Vacuolar ATP synthase subunit D antibody; Vacuolar proton pump subunit D antibody; VATD antibody; VMA8 antib
Specifications & Purity
ExactAb™, Validated, Carrier Free, High performance, 0.9 mg/mL
Host species
Rabbit
Specificity
ATP6V1D
Immunogen
Fusion protein of human ATP6V1D (AA 169-238).
Positive Control
WB: Mouse brain tissue lysate, NIH/3T3, C6, HeLa and HEK293 cell lysates. IHC: Human colorectal cancer tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated, 高性能
Product Description
Rabbit anti Human ATP6V1D Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/4000 IHC: 1/50-1/100 Protein Function Relevance': ""ATP6V1D is a subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.9 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 113.8 s
ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 17.7 s
ATP6V1D Antibody (Ab090177) - IHC Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer using ATP6V1D Antibody (Ab090177). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets
ATP6V1DTbio V-type proton ATPase subunit D 0 Activities
1.Qin A, Cheng TS, Pavlos NJ, Lin Z, Dai KR, Zheng MH. (2012) V-ATPases in osteoclasts: structure, function and potential inhibitors of bone resorption.. Int J Biochem Cell Biol, 44 (9):(1422-35). [PMID:22652318]
2.David, P P, Nguyen, H H, Barbier, A A and Baron, R R.. (1996) The bisphosphonate tiludronate is a potent inhibitor of the osteoclast vacuolar H(+)-ATPase.. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, [PMID:8889850]
3.Hu, R M RM and 28 more authors.. (2000) Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.. Proceedings of the National Academy of Sciences of the United States of America, (15):[PMID:10931946]
4.Chen, Yanqun Y and 11 more authors.. (2012) A SNX10/V-ATPase pathway regulates ciliogenesis in vitro and in vivo.. Cell research, [PMID:21844891]
5.Duan, Xiaohong; Yang, Shaoqing; Zhang, Lei and Yang, Tielin.. (2018) V-ATPases and osteoclasts: ambiguous future of V-ATPases inhibitors in osteoporosis.. Theranostics, [PMID:30555553]
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