Click Here for 5% Off Your First Aladdin Purchase!

ATP6V1D Antibody - Primary antibody, specific to ATP6V1D, Rabbit IgG

  • Carrier Free
  • ExactAb™
  • Validated
  • 高性能
  • 0.9 mg/mL
Features and benefits
  • Short Overview:

    pAb; Rabbit anti Human ATP6V1D Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IHC
  • WB
Item Number
Ab090177
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab090177-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab090177-50μl
50μl
In stock
$239.90
Ab090177-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$369.90
Ab090177-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

pAb; Rabbit anti Human ATP6V1D Antibody; WB, IHC; Unconjugated

Basic Description

Product NameATP6V1D Antibody - Primary antibody, specific to ATP6V1D, Rabbit IgG
SynonymsATP6M antibody; ATP6V1D antibody; V-ATPase 28 kDa accessory proteinantibody; V-ATPase subunit D antibody; V-type proton ATPase subunit D antibody; Vacuolar ATP synthase subunit D antibody; Vacuolar proton pump subunit D antibody; VATD antibody; VMA8 antib
Specifications & PurityExactAb™, Validated, Carrier Free, High performance, 0.9 mg/mL
Host speciesRabbit
SpecificityATP6V1D
ImmunogenFusion protein of human ATP6V1D (AA 169-238).
Positive ControlWB: Mouse brain tissue lysate, NIH/3T3, C6, HeLa and HEK293 cell lysates. IHC: Human colorectal cancer tissue.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated, 高性能
Product Description

Rabbit anti Human ATP6V1D Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/4000
IHC: 1/50-1/100
Protein Function
Relevance': ""ATP6V1D is a subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.9 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

ATP6V1D Antibody (Ab090177) - Western Blot
All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 113.8 s

ATP6V1D Antibody (Ab090177) - Western Blot
All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 17.7 s

ATP6V1D Antibody (Ab090177) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer using ATP6V1D Antibody (Ab090177). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

ATP6V1D Tbio V-type proton ATPase subunit D 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

1/50 - 1/100

WB

1/500-1/4000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202129Certificate of AnalysisDec 20, 2023 Ab090177
ZJ23F1202130Certificate of AnalysisDec 20, 2023 Ab090177

Related Documents

References

1. Qin A, Cheng TS, Pavlos NJ, Lin Z, Dai KR, Zheng MH.  (2012)  V-ATPases in osteoclasts: structure, function and potential inhibitors of bone resorption..  Int J Biochem Cell Biol,  44  (9): (1422-35).  [PMID:22652318]
2. David, P P, Nguyen, H H, Barbier, A A and Baron, R R..  (1996)  The bisphosphonate tiludronate is a potent inhibitor of the osteoclast vacuolar H(+)-ATPase..  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,      [PMID:8889850]
3. Hu, R M RM and 28 more authors..  (2000)  Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning..  Proceedings of the National Academy of Sciences of the United States of America,    (15):   [PMID:10931946]
4. Chen, Yanqun Y and 11 more authors..  (2012)  A SNX10/V-ATPase pathway regulates ciliogenesis in vitro and in vivo..  Cell research,      [PMID:21844891]
5. Duan, Xiaohong; Yang, Shaoqing; Zhang, Lei and Yang, Tielin..  (2018)  V-ATPases and osteoclasts: ambiguous future of V-ATPases inhibitors in osteoporosis..  Theranostics,      [PMID:30555553]

Solution Calculators