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bisBenzimide H 33342 trihydrochloride - 98%, high purity , CAS No.23491-52-3

  • ≥98%
Item Number
B113845
Grouped product items
SKUSizeAvailabilityPrice Qty
B113845-5mg
5mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$9.90
B113845-25mg
25mg
In stock
$25.90
B113845-100mg
100mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$83.90
B113845-500mg
500mg
In stock
$378.90

Discover bisBenzimide H 33342 trihydrochloride by Aladdin Scientific in 98% for only $9.90. Available - in Ligands at Aladdin Scientific. Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Tags: .

Basic Description

SynonymsHoechst 33342|23491-52-3|Bisbenzimide|Hoe 33342|Bisbenzimide ethoxide|2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazole|2,5'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-|99KZS6CNZX|2'-(4-ethoxyphenyl)-5
Specifications & Purity98%
Storage TempProtected from light,Store at -20°C
Shipped InDry ice
Product Description

Cell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258. Cell-permeable adenine-thymidine specific fluorescent stain. Useful for staining DNA, chromosomes or nuclei for fluorescence microscopy and flow cytometry applications. DNA-bound dye has an excitation maximum of 358 nm and an emission maximum of 460 nm. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H33342 and the related compound H33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10 fold greater cell-permeability than H 33258.

Associated Targets

BCL2L1 Tchem Bcl-2-like protein 1 1 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Names and Identifiers

IUPAC Name 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole
INCHI InChI=1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)
InChi Key PRDFBSVERLRRMY-UHFFFAOYSA-N
Canonical SMILES CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C
Isomeric SMILES CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C
WGK Germany 3
Alternate CAS 875756-97-1
PubChem CID 1464
Molecular Weight 561.93(anhydrous basis)
Beilstein 1234011

Certificates

Certificate of Analysis(COA)

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5 results found

Lot NumberCertificate TypeDateItem
C2004089Certificate of AnalysisDec 12, 2023 B113845
F2228081Certificate of AnalysisJun 09, 2022 B113845
F2228099Certificate of AnalysisJun 09, 2022 B113845
F2228100Certificate of AnalysisJun 09, 2022 B113845
H2426085Certificate of AnalysisNov 08, 2021 B113845

Chemical and Physical Properties

SolubilityH2O: 20 mg/mL,phosphate buffer: precipitates
Sensitivitylight sensitive

Safety and Hazards(GHS)

Pictogram(s) GHS08,   GHS07
Signal Warning
Hazard Statements

H315:Causes skin irritation

H335:May cause respiratory irritation

H341:Suspected of causing genetic defects

H302:Harmful if swallowed

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P281:Use personal protective equipment as required.

P271:Use only outdoors or in a well-ventilated area.

P270:Do not eat, drink or smoke when using this product.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P403+P233:Store in a well-ventilated place. Keep container tightly closed.

P362+P364:Take off contaminated clothing and wash it before reuse.

P330:Rinse mouth.

P203:Obtain, read and follow all safety instructions before use.

P301+P317:IF SWALLOWED: Get medical help.

P318:if exposed or concerned, get medical advice.

P332+P317:If skin irritation occurs: Get medical help.

P319:Get medical help if you feel unwell.

WGK Germany 3

Related Documents

References

1. Fahkrajang W et al..  (2021)  Bovine respiratory coronavirus enhances bacterial adherence by upregulating expression of cellular receptors on bovine respiratory epithelial cells..  Vet Microbiol,  255  (109017).  [PMID:33639390]
2. Sudaryatma PE et al..  (2020)  Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells..  Vet Microbiol,  246  (108748).  [PMID:32605748]
3. Sudaryatma PE et al..  (2019)  Co-infection of epithelial cells established from the upper and lower bovine respiratory tract with bovine respiratory syncytial virus and bacteria..  Vet Microbiol,  235  (80-85).  [PMID:31282382]
4. McCulloch A et al..  (2020)  Endothelialized silicone aneurysm models for in vitro evaluation of flow diverters..  J Neurointerv Surg,      [PMID:33106319]
5. Lorenzoni M et al..  (2022)  ETS-related gene (ERG) undermines genome stability in mouse prostate progenitors via Gsk3β dependent Nkx3.1 degradation..  Cancer Lett,  534  (215612).  [PMID:35259458]
6. Cambuli F et al..  (2022)  Intra-epithelial non-canonical Activin A signaling safeguards prostate progenitor quiescence..  EMBO Rep,  23  (5): (e54049).  [PMID:35253958]
7. Pazinato FM et al..  (2019)  Immunolocalization of Leptin and its Receptor (ObR-b) in Equine Placenta at Term and Plasma Level Measurement in the Late Gestation..  J Equine Vet Sci,  78  (1-5).  [PMID:31203970]
8. Dos Santos CJ et al..  (2018)  Impact of human cytomegalovirus on glioblastoma cell viability and chemotherapy treatment..  J Gen Virol,  99  (9): (1274-1285).  [PMID:30045780]
9. Tian NX et al..  (2018)  KChIP3 N-Terminal 31-50 Fragment Mediates Its Association with TRPV1 and Alleviates Inflammatory Hyperalgesia in Rats..  J Neurosci,  38  (7): (1756-1773).  [PMID:29335353]
10. Ming-Yan Y et al..  (2019)  Liraglutide inhibits the apoptosis of human nucleus pulposus cells induced by high glucose through PI3K/Akt/caspase-3 signaling pathway..  Biosci Rep,  39  (8):   [PMID:31383790]
11. Rodríguez-Nuevo A et al..  (2022)  Oocytes maintain ROS-free mitochondrial metabolism by suppressing complex I..  Nature,  607  (7920): (756-761).  [PMID:35859172]
12. Kumar M et al..  (2020)  Quinacrine inhibits GSTA1 activity and induces apoptosis through G1/S arrest and generation of ROS in human non-small cell lung cancer cell lines..  Oncotarget,  11  (18): (1603-1617).  [PMID:32405336]

Solution Calculators