mAb (CD01/2B7); Mouse anti Human beta 2 Microglobulin Antibody; WB, ELISA, ICC/IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab169199-10μl (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μl | In stock | $99.90 | |
| Ab169199-50μl | 50μl | In stock | $189.90 | |
| Ab169199-100μl | 100μl | In stock | $299.90 | |
| Ab169199-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
mAb (CD01/2B7); Mouse anti Human beta 2 Microglobulin Antibody; WB, ELISA, ICC/IF; Unconjugated
| Product Name | beta 2 Microglobulin Mouse mAb - Primary antibody, specific to beta 2 Microglobulin, IgG2a |
|---|---|
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | beta 2 Microglobulin |
| Immunogen | Recombinant protein corresponding to Human beta-2-microglobulin |
| Positive Control | WB: HeLa, Raji, THP-1 and HepG2 Whole cell Lysates ICC/IF: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Application |
| Isotype | IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - ICC/IF
IF analysis of beta 2 Microglobulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta 2 Microglobulin Mouse mAb (Ab169199) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| ICC/IF |
1/200
|
| WB | 1/1,000 - 1/10,000; Predicted molecular weight: 13.7 KDa |
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404432 | Certificate of Analysis | Apr 11, 2024 | Ab169199 |
| ZJ24F0404431 | Certificate of Analysis | Apr 11, 2024 | Ab169199 |
| ZJ24F0404430 | Certificate of Analysis | Apr 11, 2024 | Ab169199 |
| ZJ24R0300410 | Certificate of Analysis | Mar 29, 2024 | Ab169199 |
