Biotin-PEG3-azide is PEG derivative containing a biotin group and an azide group. The azide group can react with either alkyne moiety in Cu(I)-catalyzed Click Chemistry reaction or DBCO moiety in copper-free Click Chemistry reaction to form a stable triazole linkage. The hydrophilic PEG spacer increases solubility in aqueous media of the molecules conjugated to the biotin compound. It also helps to minimize steric hindrance involved with the binding to avidin molecules.
1.Jiang Yongpeng, Huang Jie, Tian Kai, Yi Xiao, Zheng Haonan, Zhu Yi, Guo Tiannan, Ji Xiong. (2022) Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture. GENOME BIOLOGY, 23 (1):(1-37). [PMID:36443871][10.1186/s13059-022-02812-w]
2.Zhan Yujuan, Chen Qiugu, Song Yue, Wei Xianli, Zhao Tingxiu, Chen Bonan, Li Chengxi, Zhang Wenbo, Jiang Yanjun, Tan Yuhui, Du Biaoyan, Xiao Jianyong, Wang Kun. (2022) Berbamine Hydrochloride inhibits lysosomal acidification by activating Nox2 to potentiate chemotherapy-induced apoptosis via the ROS-MAPK pathway in human lung carcinoma cells. CELL BIOLOGY AND TOXICOLOGY, (1-21). [PMID:36070022][10.1007/s10565-022-09756-8]
3.Zhai Yansheng, Huang Xiaoyan, Zhang Keren, Huang Yuchen, Jiang Yanlong, Cui Jingwei, Zhang Zhe, Chiu Cookson K. C., Zhong Weiye, Li Gang. (2022) Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling. Nature Communications, 13 (1):(1-12). [PMID:35987950][10.1038/s41467-022-32689-z]
References
1.Jiang Yongpeng, Huang Jie, Tian Kai, Yi Xiao, Zheng Haonan, Zhu Yi, Guo Tiannan, Ji Xiong. (2022) Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture. GENOME BIOLOGY, 23 (1):(1-37). [PMID:36443871][10.1186/s13059-022-02812-w]
2.Zhan Yujuan, Chen Qiugu, Song Yue, Wei Xianli, Zhao Tingxiu, Chen Bonan, Li Chengxi, Zhang Wenbo, Jiang Yanjun, Tan Yuhui, Du Biaoyan, Xiao Jianyong, Wang Kun. (2022) Berbamine Hydrochloride inhibits lysosomal acidification by activating Nox2 to potentiate chemotherapy-induced apoptosis via the ROS-MAPK pathway in human lung carcinoma cells. CELL BIOLOGY AND TOXICOLOGY, (1-21). [PMID:36070022][10.1007/s10565-022-09756-8]
3.Zhai Yansheng, Huang Xiaoyan, Zhang Keren, Huang Yuchen, Jiang Yanlong, Cui Jingwei, Zhang Zhe, Chiu Cookson K. C., Zhong Weiye, Li Gang. (2022) Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling. Nature Communications, 13 (1):(1-12). [PMID:35987950][10.1038/s41467-022-32689-z]
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