Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG4SP; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab182780-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab182780-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab182780-5mg | 5mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $749.90 | |
| Ab182780-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,189.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG4SP; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | Bleselumab (anti-CD40) - Primary antibody, specific to CD40, Human IgG4SP |
|---|---|
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Specificity | CD40 |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
| Action Type | INHIBITOR |
| Mechanism of action | INHIBITOR of Tumor necrosis factor receptor superfamily member 5 inhibitor |
| Product Description | Bleselumab (anti-CD40) is a human anti-CD40 monoclonal antibody (mAb). Bleselumab (anti-CD40) binds to human CD40 with high affinity (Kd: 0.24 nM). Bleselumab (anti-CD40) inhibits immune responses by blocking the interaction of CD40 with its ligand CD40L. Bleselumab (anti-CD40) prevents organ transplant rejection. Purity >95% (SDS-PAGE&SEC) Endotoxin Level < 1.0EU/mg |
| Isotype | Human IgG4SP |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 25.8 kDa (Light Chain) & 53.0 kDa (Heavy Chain), under reducing conditions; 186.9 kDa, under non-reducing conditions. |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| CAS | 1453067-91-8 |
Bleselumab (anti-CD40) (Ab182780) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 with Bleselumab (anti-CD40) (Ab182780) conjugated with biotin (right panel) compared with Human IgG (Biotin) (Ab180314) - Isotype Control (left panel) and detected with SA-PE (rp156150).
Bleselumab (anti-CD40) (Ab182780) - SEC
The purity of Bleselumab (anti-CD40) (Ab182780) is more than 95% verified by HPLC.
Bleselumab (anti-CD40) (Ab182780) - ELISA
Immobilized Recombinant Human CD40 protein (rp144000) at 1.0 μg/mL can bind Bleselumab (anti-CD40) (Ab182780) with the EC50 of 27.51 ng/mL.
Bleselumab (anti-CD40) (Ab182780) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD40 (red) with Bleselumab (anti-CD40) (Ab182780). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Bleselumab (anti-CD40) (Ab182780) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 (red) with Bleselumab (anti-CD40) (Ab182780) conjugated with biotin and detected with SA-PE (rp156150). Blue - Isotype control, Human IgG (Biotin) (Ab180314). Black - Unlabelled control, cells without incubation with primary antibody.
Bleselumab (anti-CD40) (Ab182780) - Flow Cytometry
Flow Cytometry analysis of U-2 OS cells labelling CD40 (red) with Bleselumab (anti-CD40) (Ab182780). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505999 | Certificate of Analysis | May 23, 2024 | Ab182780 |
| ZJ24F0505998 | Certificate of Analysis | May 23, 2024 | Ab182780 |
| ZJ24F0505997 | Certificate of Analysis | May 23, 2024 | Ab182780 |
