All Blue Protein MW Marker (10-250kDa)

Item Number

rp210256

• Product Name: All Blue Protein MW Marker (10-250kDa)
• SDS-PAGE: 10/15/20/25/30/40/50/70/100/130/180/250 kDa

Product Description

Color pre dyed protein molecular weight standard:
1. This product contains 12 highly purified and pre stained recombinant proteins ranging from 10kDa to 250kDa.
2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting.
3. This color pre stained protein molecular weight standard has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents.
4. According to the size of the sample well, this color pre stained protein molecular weight standard usually requires 5-10 microliters of sample per loading (5μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer.
Usage method:
1. After thawing at room temperature, completely dissolve and gently mix well, do not boil.
2. Take 5µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images.
3. Unused color pre dyed protein molecular weight standards should be stored under storage conditions and can be left at 4°C for 2 months.
Matters needing attention:
1. In low concentration gels, low molecular weight proteins will swim at the front edge of the dye.
2. When transferring high molecular weight proteins, it is recommended to extend the transfer time or increase the transfer voltage. If the effect is not good, it is recommended to adjust the transfer solution formula, reduce the amount of methanol used, and add a small amount of SDS (final concentration not exceeding 0.04%).
3. Pre stained proteins have different apparent molecular weights in different buffer systems. If non pre stained proteins are calibrated in advance in this buffer system, the protein molecular weight can be roughly determined.