| Product Description | Aladdin's BODIPY 500/510 C1, C12 (Green-fluorescent Fatty Acid Probe) is a fluorescence-labelled fatty acid. It is cell membrane permeable and can specifically stain polar lipids in cells. It is often used as a fluorescent probe to stain cell membranes or to investigate fatty acid uptake, lipid transport, intestinal lipid uptake, lipid uptake and their accumulation in tissues such as fat and liver. It can also be used as a precursor for the synthesis of a variety of fluorescent phospholipids. Its fluorescence can be examined by fluorescence microscopy, confocal microscopy, flow cytometry, and fluorescence plate reader, etc.BODIPY (4,4-Difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid) is a uncharged and neutral small molecule dye with strong UV absorption, low inter-dye interference, narrow spectral bandwidth, relatively sharp fluorescence peaks, and high fluorescence quantum yield. Unlike most other long-wavelength dyes, BODIPY is lipophilic and can mimic the properties of natural lipids. It is relatively stable under different physiological conditions and insensitive to the polarity and pH of the environment. Due to its asymmetric structure, there are a variety of derived structural products, of which BODIPY 500/510 C1, C12 is one. The molecular formula of BODIPY 500/510 C1, C12 is C22H31BF2N2O2 with a molecular weight of 404.3. When doped into living cells, it can form excimers and emit red-shifted fluorescence, with the excitation and emission maxima at 500nm and 510nm, respectively.Lipids are a group of compounds that are insoluble in water but soluble in organic solvents such as ethanol, ether, and benzene. In animals, lipids mainly include fats, phospholipids, and steroids. Defects in lipid processing and transport are the basis of many metabolic diseases, and abnormalities in lipid metabolism are also major factors leading to obesity, diabetes, cardiovascular diseases, and carcinogenesis. By labeling fatty acids with different chain lengths, the transport and metabolism of fatty acids can be visualized, which can provide better understanding of metabolic functions [1]. BODIPY 500/510 C1, C12 is commonly used as a fluorescent probe for studying fatty acid uptake and lipid transport. For example, it can be dissolved in olive oil to detect the absorption of fatty acids in the intestine of mice, assess the absorption of fatty acids in liver and adipose tissues after gavage, and investigate lipid transport in vascular smooth muscles to evaluate the role of lipotoxicity in the development of atherosclerosis [2].Please refer to Figure 1 for the performance of this product in staining cellular lipids.Figure 1. NRK-52 cells stained with Aladdin's BODIPY 500/510 C1, C12 (Green-fluorescent Fatty Acid Probe) . A. Morphology of normal NRK-52 cells under brightfield; B. Labeling of normal cell membrane with BODIPY 500/510 C1, C12, and no obvious lipid droplet aggregation was observed within the normal cells; E. NRK-52 cells treated with oleic acid, with significant aggregation of lipid droplets in cells; F. Lipid droplets labeled with BODIPY 500/510 C1, C12. C, D. Cell nuclei stained with DAPI . This figure is for reference only, which may vary due to different experimental conditions.The recommended starting working concentration for BODIPY 500/510 C1, C12 is 1-10µM. The recommended dosage for gavage in mice is 0.5µg/g body weight. For cell staining, we recommend using DMSO as the solvent, but for gavage in animals such as mice, Olive Oil is recommended.
Precautions： Prepare the stock solution in appropriate solvents as required, such as DMSO and Olive Oil. Stock solutions in DMSO can be stored at -20℃ in the dark for up to one month, or -80℃ for longer periods.Fluorescent dyes are susceptible to quenching. Please avoid light exposure to minimize fluorescence quenching.This product should be used aseptically to avoid any potential impact on the assay results.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.1. Carten JD, Bradford MK, Farber SA. Dev Biol. 2011. 360 (2):276-285.2. Mattern HM, Hardin CD. Physiol Res. 2007. 56 (2):149-158.
Instructions for Use： none
|
|---|
