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Bovine Thrombin

20 Citations
Features and benefits
    Item Number
    B741825
    Grouped product items
    SKUSizeAvailabilityPrice Qty
    B741825-6500US
    		6500US unit
    Available within 8-12 weeks(?)
    Production requires sourcing of materials. We appreciate your patience and understanding.
    $619.90
    Add to Wish List Compare
    Bulk Order  DATASHEET

    Basic Description

    Product NameBovine Thrombin
    GradeHigh Purity
    Product Description

    TestSpecification
    Thrombin Activity (by coagulation)> 200,000 US units/g powder
    Protein (by Biuret)Not Specified
    Thrombin Specific Activity> 1500 US units/mg powder
    Moisture0 - 5%
    pH (1% solution)6.5 - 7.5
    Salt EnvironmentMannitol, Sodium Chloride
    Recommended diluent for reconstitution0.9% NaCl

    This product is for in vitro (diagnostic) use only. Not for human or animal consumption.

    The high purity bovine thrombin (> 1500 U/mg protein) is used by many diagnostic companies worldwide to defibrinate plasma, producing serum matrixes to be used as controls and standards or in polyclonal antibody production. Using bovine thrombin in polyclonal antibody production to defibrinate plasma may increase antibody yields by 15-20% over off-the-clot methods of defibrination.

    Our high purity thrombin is also used to cleave His Tags and GST Tags with a high degree of specificity. We express our units in US Units (aka, NIH Units) measured by coagulation. There are approximately 5 Thrombin cleavage units for every one US Unit.
    Specifications & PurityHigh Purity, > 1500 U/Mg Protein

    Product Properties

    NacresNA.54

    Mechanisms of Action

    Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

    Product Specifications

    Concentration> 1500 U/Mg Protein
    CAS9002-04-4
    Enzyme Commission Number3.4.21.5 (BRENDA, IUBMB)

    Certificates

    Certificate of Analysis(COA)

    Enter Lot Number to search for COA:

    Safety and Hazards(GHS)

    Pictogram(s) GHS08,   GHS07
    Signal Danger
    Hazard Statements

    H315:Causes skin irritation

    H319:Causes serious eye irritation

    H335:May cause respiratory irritation

    H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled

    Precautionary Statements

    P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

    P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

    P280:Wear protective gloves/protective clothing/eye protection/face protection.

    P302+P352:IF ON SKIN: wash with plenty of water.

    P321:Specific treatment (see ... on this label).

    P405:Store locked up.

    P501:Dispose of contents/container to ...

    P264:Wash hands [and …] thoroughly after handling.

    P284:[In case of inadequate ventilation] Wear respiratory protection.

    P271:Use only outdoors or in a well-ventilated area.

    P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

    P403+P233:Store in a well-ventilated place. Keep container tightly closed.

    P362+P364:Take off contaminated clothing and wash it before reuse.

    P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

    P337+P317:If eye irritation persists: Get medical help.

    P332+P317:If skin irritation occurs: Get medical help.

    P319:Get medical help if you feel unwell.

    P342+P316:If experiencing respiratory symptoms: Get emergence medical help immediately.

    Related Documents

     SDS

     Specification Sheet

    Citations of This Product

    How to Cite Aladdin Scientific Products?
    1. Zhechang Chen, Caijie Yuan, Yaxin Ye, Bitao Lu, Enling Hu, Fei Lu, Kun Yu, Ruiqi Xie, Guangqian Lan.  (2024)  Dual-targeting fucoidan-based microvesicle for arterial thrombolysis and re-occlusion inhibition.  CARBOHYDRATE POLYMERS,  328  (121703).  [PMID:38220339] [10.1016/j.carbpol.2023.121703]
    2. Hong Liu, Xuliang Yang, Bo Huang, Hongxiang Liu.  (2023)  A universal approach for synthesis of copper nanoclusters templated by G-rich oligonucleotide sequences and their applications in sensing.  SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,  297  (122740).  [PMID:37080047] [10.1016/j.saa.2023.122740]
    3. Xinxin Ren, Qingqing Zhang, Lingxia Qin, Kaiyue Hu, Zhiyong Guo, Sui Wang, Yufang Hu.  (2023)  Checkerboard arranged G4 nanostructure-supported electrochemical platform and its application to unique bio-enzymes examination.  BIOELECTROCHEMISTRY,  149  (108282).  [PMID:36242949] [10.1016/j.bioelechem.2022.108282]
    4. Xinxin Ren, Kaiyue Hu, Lingxia Qin, Di Wu, Zhiyong Guo, Sui Wang, Yufang Hu.  (2022)  Development of ZnO nanoflowers-assisted DNAzyme-based electrochemical platform for invertase and glucose oxidase-dominated biosensing.  ANALYTICA CHIMICA ACTA,  1232  (340438).  [PMID:36257731] [10.1016/j.aca.2022.340438]
    5. Songzhi Xie, Chuanfei Mo, Wenxiong Cao, Shuang Xie, Shang Li, Zhanlin Zhang, Xiaohong Li.  (2022)  Bacteria-propelled microtubular motors for efficient penetration and targeting delivery of thrombolytic agents.  Acta Biomaterialia,  142  (49).  [PMID:35158079] [10.1016/j.actbio.2022.02.008]
    6. Fuad Abduro Bushira, Shimeles Addisu Kitte, Yong Wang, Haijuan Li, Ping Wang, Yongdong Jin.  (2021)  Plasmon-Boosted Cu-Doped TiO2 Oxygen Vacancy-Rich Luminol Electrochemiluminescence for Highly Sensitive Detection of Alkaline Phosphatase.  ANALYTICAL CHEMISTRY,  93  (45): (15183–15191).  [PMID:34743510] [10.1021/acs.analchem.1c03842]
    7. Qing Zhu, Ping Ye, Fang Guo, Yimen Zhu, Wenbin Nan, Zhaohua Chang.  (2021)  A heparin-functionalized covered stent prepared by plasma technology.  JOURNAL OF BIOMATERIALS APPLICATIONS,      [PMID:34672223] [10.1177/08853282211051871]
    8. Liu Shanshan, Zheng Sitian, Chu Jing.  (2021)  Cationic Polythiophene-based Colorimetric Assay for Probing the Activity of Protein Kinase A.  ANALYTICAL SCIENCES,  37  (7): (1039-1043).  [PMID:33250451] [10.2116/analsci.20N034]
    9. Zheng Di-Wei, Hong Sheng, Zhang Qiu-Ling, Dong Xue, Pan Pei, Song Wen-Fang, Song Wen, Cheng Si-Xue, Zhang Xian-Zheng.  (2020)  Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies.  Nature Communications,  11  (1): (1-15).  [PMID:32999289] [10.1038/s41467-020-18493-7]
    10. Zhang Hong, Liu Pengfei, Wang Huifang, Ji Xiaoming, Zhao Mingqin, Song Zhaopeng.  (2021)  Label-free fluorescent sensor for one-step lysozyme detection via positively charged gold nanorods.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  413  (6): (1541-1547).  [PMID:32705288] [10.1007/s00216-020-02814-2]
    11. Liping Lin, Yaxin Luo, Qiushuang Chen, Qingjiao Lai, Qiaoling Zheng.  (2020)  Redox-modulated colorimetric detection of ascorbic acid and alkaline phosphatase activity with gold nanoparticles.  LUMINESCENCE,  35  (4): (542-549).  [PMID:31898408] [10.1002/bio.3749]
    12. Ding Wang, Yaqin Chai, Yali Yuan, Ruo Yuan.  (2020)  Lattice-Like DNA Tetrahedron Nanostructure as Scaffold to Locate GOx and HRP Enzymes for Highly Efficient Enzyme Cascade Reaction.  ACS Applied Materials & Interfaces,  12  (2): (2871–2877).  [PMID:31849211] [10.1021/acsami.9b18702]
    13. Wang Jing, Li Bingzhi, Lu Qiaoyun, Li Xiaoyun, Weng Chenyuan, Yan Xiaoqiang, Hong Junli, Zhou Xuemin.  (2019)  A versatile fluorometric aptasensing scheme based on the use of a hybrid material composed of polypyrrole nanoparticles and DNA-silver nanoclusters: application to the determination of adenosine, thrombin, or interferon-gamma.  MICROCHIMICA ACTA,  186  (6): (1-8).  [PMID:31098714] [10.1007/s00604-019-3459-9]
    14. Fangjing Mo, Jingling Wu, Min Chen, Hui Meng, Qian Han, Yingzi Fu.  (2019)  Enzyme-free “on-off-on” photoelectrochemical biosensor based on cascaded quadratic amplification strategy for miRNA 141 detection.  SENSORS AND ACTUATORS B-CHEMICAL,  289  (269).  [PMID:] [10.1016/j.snb.2019.03.044]
    15. Shuang Tan, Rui Han, Shilian Wu, Huan Liang, Yuting Zhao, Hui Zhao, Can-Peng Li.  (2019)  A novel fluorescent sensing platform for insulin detection based on competitive recognition of cationic pillar[6]arene.  TALANTA,  197  (130).  [PMID:30771914] [10.1016/j.talanta.2019.01.004]
    16. Shaohua Ma, Yufang Hu, Qingqing Zhang, Zhiyong Guo, Sui Wang, Qingpeng Shen, Chunbo Liu, Zhihua Liu.  (2018)  Adenine/Au complex-dependent versatile electrochemical platform for ultrasensitive DNA-related enzyme activity assay.  SENSORS AND ACTUATORS B-CHEMICAL,  273  (760).  [PMID:] [10.1016/j.snb.2018.06.115]
    17. Mao Mi, Tian Tian, He Yu, Ge Yili, Zhou Jiangang, Song Gongwu.  (2018)  Inner filter effect based fluorometric determination of the activity of alkaline phosphatase by using carbon dots codoped with boron and nitrogen.  MICROCHIMICA ACTA,  185  (1): (1-6).  [PMID:29594532] [10.1007/s00604-017-2541-4]
    18. Yufang Hu, Qingqing Zhang, Zhiyong Guo, Sui Wang, Chunnuan Du, Chunyang Zhai.  (2017)  In situ grown DNA nanotail-templated silver nanoclusters enabling label-free electrochemical sensing of terminal deoxynucleotidyl transferase activity.  BIOSENSORS & BIOELECTRONICS,  98  (91).  [PMID:28662471] [10.1016/j.bios.2017.06.017]
    19. Xiangying Sun, Bin Liu, Chuanxiao Yang, Congcong Li.  (2014)  An extremely sensitive aptasensor based on interfacial energy transfer between QDS SAMs and GO.  SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,  131  (288).  [PMID:24835931] [10.1016/j.saa.2014.04.093]
    20. Ting Cheng,Xiang Li,Peng Huang,Han Wang,Meixia Wang,Wenming Yang.  (2019-01-12)  Colorimetric and electrochemical (dual) thrombin assay based on the use of a platinum nanoparticle modified metal-organic framework (type Fe-MIL-88) acting as a peroxidase mimic..  Mikrochimica acta,  186  ((2)): (94-94).  [PMID:30631938]

    References

    1. Zhechang Chen, Caijie Yuan, Yaxin Ye, Bitao Lu, Enling Hu, Fei Lu, Kun Yu, Ruiqi Xie, Guangqian Lan.  (2024)  Dual-targeting fucoidan-based microvesicle for arterial thrombolysis and re-occlusion inhibition.  CARBOHYDRATE POLYMERS,  328  (121703).  [PMID:38220339] [10.1016/j.carbpol.2023.121703]
    2. Hong Liu, Xuliang Yang, Bo Huang, Hongxiang Liu.  (2023)  A universal approach for synthesis of copper nanoclusters templated by G-rich oligonucleotide sequences and their applications in sensing.  SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,  297  (122740).  [PMID:37080047] [10.1016/j.saa.2023.122740]
    3. Xinxin Ren, Qingqing Zhang, Lingxia Qin, Kaiyue Hu, Zhiyong Guo, Sui Wang, Yufang Hu.  (2023)  Checkerboard arranged G4 nanostructure-supported electrochemical platform and its application to unique bio-enzymes examination.  BIOELECTROCHEMISTRY,  149  (108282).  [PMID:36242949] [10.1016/j.bioelechem.2022.108282]
    4. Xinxin Ren, Kaiyue Hu, Lingxia Qin, Di Wu, Zhiyong Guo, Sui Wang, Yufang Hu.  (2022)  Development of ZnO nanoflowers-assisted DNAzyme-based electrochemical platform for invertase and glucose oxidase-dominated biosensing.  ANALYTICA CHIMICA ACTA,  1232  (340438).  [PMID:36257731] [10.1016/j.aca.2022.340438]
    5. Songzhi Xie, Chuanfei Mo, Wenxiong Cao, Shuang Xie, Shang Li, Zhanlin Zhang, Xiaohong Li.  (2022)  Bacteria-propelled microtubular motors for efficient penetration and targeting delivery of thrombolytic agents.  Acta Biomaterialia,  142  (49).  [PMID:35158079] [10.1016/j.actbio.2022.02.008]
    6. Fuad Abduro Bushira, Shimeles Addisu Kitte, Yong Wang, Haijuan Li, Ping Wang, Yongdong Jin.  (2021)  Plasmon-Boosted Cu-Doped TiO2 Oxygen Vacancy-Rich Luminol Electrochemiluminescence for Highly Sensitive Detection of Alkaline Phosphatase.  ANALYTICAL CHEMISTRY,  93  (45): (15183–15191).  [PMID:34743510] [10.1021/acs.analchem.1c03842]
    7. Qing Zhu, Ping Ye, Fang Guo, Yimen Zhu, Wenbin Nan, Zhaohua Chang.  (2021)  A heparin-functionalized covered stent prepared by plasma technology.  JOURNAL OF BIOMATERIALS APPLICATIONS,      [PMID:34672223] [10.1177/08853282211051871]
    8. Liu Shanshan, Zheng Sitian, Chu Jing.  (2021)  Cationic Polythiophene-based Colorimetric Assay for Probing the Activity of Protein Kinase A.  ANALYTICAL SCIENCES,  37  (7): (1039-1043).  [PMID:33250451] [10.2116/analsci.20N034]
    9. Zheng Di-Wei, Hong Sheng, Zhang Qiu-Ling, Dong Xue, Pan Pei, Song Wen-Fang, Song Wen, Cheng Si-Xue, Zhang Xian-Zheng.  (2020)  Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies.  Nature Communications,  11  (1): (1-15).  [PMID:32999289] [10.1038/s41467-020-18493-7]
    10. Zhang Hong, Liu Pengfei, Wang Huifang, Ji Xiaoming, Zhao Mingqin, Song Zhaopeng.  (2021)  Label-free fluorescent sensor for one-step lysozyme detection via positively charged gold nanorods.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  413  (6): (1541-1547).  [PMID:32705288] [10.1007/s00216-020-02814-2]
    11. Liping Lin, Yaxin Luo, Qiushuang Chen, Qingjiao Lai, Qiaoling Zheng.  (2020)  Redox-modulated colorimetric detection of ascorbic acid and alkaline phosphatase activity with gold nanoparticles.  LUMINESCENCE,  35  (4): (542-549).  [PMID:31898408] [10.1002/bio.3749]
    12. Ding Wang, Yaqin Chai, Yali Yuan, Ruo Yuan.  (2020)  Lattice-Like DNA Tetrahedron Nanostructure as Scaffold to Locate GOx and HRP Enzymes for Highly Efficient Enzyme Cascade Reaction.  ACS Applied Materials & Interfaces,  12  (2): (2871–2877).  [PMID:31849211] [10.1021/acsami.9b18702]
    13. Wang Jing, Li Bingzhi, Lu Qiaoyun, Li Xiaoyun, Weng Chenyuan, Yan Xiaoqiang, Hong Junli, Zhou Xuemin.  (2019)  A versatile fluorometric aptasensing scheme based on the use of a hybrid material composed of polypyrrole nanoparticles and DNA-silver nanoclusters: application to the determination of adenosine, thrombin, or interferon-gamma.  MICROCHIMICA ACTA,  186  (6): (1-8).  [PMID:31098714] [10.1007/s00604-019-3459-9]
    14. Fangjing Mo, Jingling Wu, Min Chen, Hui Meng, Qian Han, Yingzi Fu.  (2019)  Enzyme-free “on-off-on” photoelectrochemical biosensor based on cascaded quadratic amplification strategy for miRNA 141 detection.  SENSORS AND ACTUATORS B-CHEMICAL,  289  (269).  [PMID:] [10.1016/j.snb.2019.03.044]
    15. Shuang Tan, Rui Han, Shilian Wu, Huan Liang, Yuting Zhao, Hui Zhao, Can-Peng Li.  (2019)  A novel fluorescent sensing platform for insulin detection based on competitive recognition of cationic pillar[6]arene.  TALANTA,  197  (130).  [PMID:30771914] [10.1016/j.talanta.2019.01.004]
    16. Shaohua Ma, Yufang Hu, Qingqing Zhang, Zhiyong Guo, Sui Wang, Qingpeng Shen, Chunbo Liu, Zhihua Liu.  (2018)  Adenine/Au complex-dependent versatile electrochemical platform for ultrasensitive DNA-related enzyme activity assay.  SENSORS AND ACTUATORS B-CHEMICAL,  273  (760).  [PMID:] [10.1016/j.snb.2018.06.115]
    17. Mao Mi, Tian Tian, He Yu, Ge Yili, Zhou Jiangang, Song Gongwu.  (2018)  Inner filter effect based fluorometric determination of the activity of alkaline phosphatase by using carbon dots codoped with boron and nitrogen.  MICROCHIMICA ACTA,  185  (1): (1-6).  [PMID:29594532] [10.1007/s00604-017-2541-4]
    18. Yufang Hu, Qingqing Zhang, Zhiyong Guo, Sui Wang, Chunnuan Du, Chunyang Zhai.  (2017)  In situ grown DNA nanotail-templated silver nanoclusters enabling label-free electrochemical sensing of terminal deoxynucleotidyl transferase activity.  BIOSENSORS & BIOELECTRONICS,  98  (91).  [PMID:28662471] [10.1016/j.bios.2017.06.017]
    19. Xiangying Sun, Bin Liu, Chuanxiao Yang, Congcong Li.  (2014)  An extremely sensitive aptasensor based on interfacial energy transfer between QDS SAMs and GO.  SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,  131  (288).  [PMID:24835931] [10.1016/j.saa.2014.04.093]
    20. Ting Cheng,Xiang Li,Peng Huang,Han Wang,Meixia Wang,Wenming Yang.  (2019-01-12)  Colorimetric and electrochemical (dual) thrombin assay based on the use of a platinum nanoparticle modified metal-organic framework (type Fe-MIL-88) acting as a peroxidase mimic..  Mikrochimica acta,  186  ((2)): (94-94).  [PMID:30631938]

    Solution Calculators

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