ExactAb™, Validated, Carrier Free, Azide Free, High performance, 0.5 mg/mL
Host species
Mouse
Immunogen
Bromodeoxyuridine
Positive Control
Flow: Jurkat cells; ICC/IF: HeLa cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Note
20μl卖完停产，不再备货
Product Description
Application Flow: 0.16 μg/mL - 5 μg/mL ICC/IF: 0.5 μg/mL Mouse anti BrdU antibody, clone 2F8, is suitable for detecting incorporated BrdU in a wide variety of cell types.
Product Properties
Isotype
IgG1
Light Chain Type
lambda
Purification Method
Protein A purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
BrdU Mouse mAb (Ab179917) - Flow Cytometry Dot plot showing BrdU-treated (A) and DMSO-treated (B) Jurkat cells stained with Ab179917. Cells were incubated with 10 µM BrdU for 1 hour prior to being harvested. And then fixed in 100% methanol for 20 minutes at -20℃. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature. Cells were stained with 0.32 μg/mL BrdU Mouse mAb (Ab179917) for 1 hour at room temperature. Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as a secondary antibody at a 1/1000 dilution. Propidium Iodide was used to stain total DNA.
BrdU Mouse mAb (Ab179917) - ICC/IF IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab179917) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab179917) at 0.5 μg/mL. beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue). Images were taken on the confocal laser scanning microscope.
Application
Application
Dilution info
IF/ICC
0.5 μg/mL
Flow Cytometry
0.16 μg/mL - 5.0 μg/mL
Certificates
Certificate of Analysis(COA)
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ZJ23F1202282