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B196451-100ml

100ml

Available within 4-8 weeks(?)

$20.90

B196451-500ml

500ml

Available within 4-8 weeks(?)

$79.90

Basic Description

Synonyms	Bromophenol Blue, ACS reagent \| Bromophenol Blue, ACS reagent, EP \| bromophenol blue, sultone form \| Tetrabromphenol Blue \| CAS-115-39-9 \| CS-0013463 \| FT-0614001 \| CHEBI:59424 \| Bromophenol blue indicator (3.0-4.6) \| 4,4'-(1,1-Dioxido-3H-2,1-benzoxathiol
Specifications & Purity	0.05%(W/V)in water
Shipped In	Normal
Product Description	Bromophenol blue is useful as a tracking dye in electrophoresis, an industrial dye, a laboratory acid-base indicator and a biological stain. In gel electrophoresis it can be used as a color marker and as as a biological stain it can be used to stain proteins and nucleic acids. Bromophenol blue is an intermediate and is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue. An electrophoresis tracking dye also used as an acid-base indicator

Associated Targets(non-human)

Cavia porcellus (23802 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Names and Identifiers

IUPAC Name	2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]phenol
INCHI	InChI=1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
InChi Key	UDSAIICHUKSCKT-UHFFFAOYSA-N
Canonical SMILES	C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC(=C(C(=C3)Br)O)Br)C4=CC(=C(C(=C4)Br)O)Br
Isomeric SMILES	C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC(=C(C(=C3)Br)O)Br)C4=CC(=C(C(=C4)Br)O)Br
WGK Germany	2
RTECS	SJ7453000
PubChem CID	8272
Molecular Weight	669.96
Beilstein	61698
Reaxy-Rn	61698

Certificates

Certificate of Analysis(COA)

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Chemical and Physical Properties

Melt Point(°C)	297°C

Safety and Hazards(GHS)

WGK Germany	2
RTECS	SJ7453000
Reaxy-Rn	61698
Merck Index	1444

FAQs and Articles

FAQ

Methods of Lysate Preparation for Western Blotting

Categories: Protocols

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Western blotting SDS Cell lysis Tissue dissociation Protein extraction RIPA buffer

Citations of This Product

1. Xianheng Li, Huan Li, Keqiang Lai, Junjian Miao, Jie Zhao. (2024) New natural deep eutectic solvent for vortex-assisted liquid–liquid microextraction for the determination of bisphenols in beverages. MICROCHEMICAL JOURNAL, 197 (18): (109710). [PMID:26382858]
2. Wang Tingting, Yue Xiaoju, Han Lin, Wang Jinnong, Zhang Yuzhen, Tang Xiaofeng, Wang Shifeng. (2023) Synthesis of UiO-66-NH2@SiO2 with a multistage pore structure for effective adsorption of organic pollutants. International Journal of Low-Carbon Technologies, 18 (1284-1295). [PMID:9466265]
3. Zhihui Yu, Li Ma, Binbin Liu, Wenqing Wang, Ziqi Shang, Huichao Dang, Chunyou Liu. (2023) Improvement of foaming properties of ovalbumin: Insights into the synergistic effect of preheating and high-intensity ultrasound on physicochemical properties and structure analysis. ULTRASONICS SONOCHEMISTRY, 101 (106672). [PMID:37925915]
4. Huimin Shi, Yang Li, Kailei Xu, Jun Yin. (2023) Advantages of photo-curable collagen-based cell-laden bioinks compared to methacrylated gelatin (GelMA) in digital light processing (DLP) and extrusion bioprinting. Materials Today Bio, 23 (21): (100799). [PMID:37766893]
5. Xiang-Yu Wang, Ming-Wei Zhao, Xu-Hao Wang, Peng Liu, Meng-Yao Fan, Teng Li, Zhen-Feng Ma, Ying-Jie Dai, Cai-Li Dai. (2023) Intermolecular interactions induced property improvement for clean fracturing fluid by deep eutectic solvents. Petroleum Science, [PMID:]
6. Xue Liu, Li Wang, Bowen He, Qiao Liu, He Zhu, Andrew J. Carrier, Ken D. Oakes, Xu Zhang. (2023) Mechanistic Insights into Myofibrillar Protein Oxidation by Fenton Chemistry Regulated by Gallic Acid. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 71 (33): (12587–12596). [PMID:37561819]
7. Ting Zeng, Jie Yin, Ziwen Liu, Zhaoxing Li, Yu Zhang, Yang Lv, Mei-Ling Lu, Min Luo, Meirong Chen, Yibei Xiao. (2023) Mechanistic insights into transposon cleavage and integration by TnsB of ShCAST system. Cell Reports, 42 (112698). [PMID:37379212]
8. Jie Wang, Chunyan Wu, Xing Zhang, Yuanyuan Song, Binquan Wang, Ke Zhang, Mingkuan Sun. (2023) Developmental neurotoxic effects of bisphenol A and its derivatives in Drosophila melanogaster. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 260 (115098). [PMID:37269611]
9. Guorong Ma, Pengzheng Luo, Ruiqiang Xu, Rui Ma, Lei Qiu, Chenran Xu, Rang Yang, Yating Li, Zhihao Zhao, Ling Huang, Yanhui Yang, Pei Wang. (2023) Improving the profile and reliability of cytoplasmic proteins from M. tuberculosis using biomimetic affinity chromatography coupled with LC-MS/MS analysis. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1225 (123756). [PMID:37236071]
10. Xiqing Liu, Tao Wang, Yongqing Wang. (2023) Selective and ratiometric fluorescence sensing of bisphenol A in canned food based on portable fluorescent test strips. ANALYTICA CHIMICA ACTA, 1240 (340728). [PMID:36641151]
11. Hongfeng Li, Haohao Shi, Xinyi Chen, Zhen Ren, Yu Shen, Peng Wu, Yun Fan, Xinglong Zhang, Wenxiong Shi, Honggang Liao, Suoying Zhang, Weina Zhang, Fengwei Huo. (2023) Construction of Metal–Organic Framework Films via Crosslinking-Induced Assembly. ADVANCED MATERIALS, 35 (9): (2209777). [PMID:36493462]
12. Shixin Song, Yufei Li, Qiyuan Zhu, Xin Zhang, Yang Wang, Li Tao, Lei Yu. (2023) Structure and properties of Pickering emulsions stabilized solely with novel buckwheat protein colloidal particles. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 226 (61). [PMID:36493922]
13. Qingqing Yu, Tong Shi, Zhiyu Xiong, Li Yuan, Hui Hong, Ruichang Gao, Yulong Bao. (2023) Oxidation affects dye binding of myofibrillar proteins via alteration in net charges mediated by a reduction in isoelectric point. FOOD RESEARCH INTERNATIONAL, 163 (112204). [PMID:36596136]
14. Xin Luo, Jiankang Lu, Yongyan Wu, Wenshan Duan, Fengping An, Qun Huang, Lei Chen, Shaofeng Wei. (2022) Reducing the potential allergenicity of amandin through binding to (−)-epigallocatechin gallate. Food Chemistry-X, 16 (100482). [PMID:36304206]
15. Jianchao Gong, Tingting Wang, Wei Zhang, Lin Han, Mingxiao Gao, Tianen Chen, Tao Shen, Yaxiong Ji. (2022) Organo-Vermiculites Modified by Aza-Containing Gemini Surfactants: Efficient Uptake of 2-Naphthol and Bromophenol Blue. Nanomaterials, 12 (20): (3636). [PMID:36296825]
16. Hui Liu, Mengya Li, Keyong Tang, Jie Liu, Xiumin Li, Xueping Meng. (2022) Evolution of conformation and thermal properties of bovine hides collagen in the sodium sulphide solution. JOURNAL OF MOLECULAR LIQUIDS, 367 (120449). [PMID:]
17. Lian Xia, Yuxia Yang, Hongqin Yang, Yue Tang, Jianli Zhou, Yuangen Wu. (2022) Screening and identification of an aptamer as novel recognition molecule in the test strip and its application for visual detection of ethyl carbamate in liquor. ANALYTICA CHIMICA ACTA, 1226 (340289). [PMID:36068048]
18. Hong Zeng, Hongli Yang, Yue Tang, Xiaojuan Niu, Yuangen Wu. (2022) Aptamer-enhanced the Ag(I) ion-3,3′,5,5′-tetramethylbenzdine catalytic system as a novel colorimetric biosensor for ultrasensitive and selective detection of paraquat. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 280 (121571). [PMID:35780760]
19. Zhi-Yong Wu, Yu-Hang Wang, Bing-Su Niu, Yu Yang, Fang Fang, Yan-Yan Song. (2022) Simultaneous enrichment and separation based on ion concentration polarization effect on a paper based analytical device. ANALYTICA CHIMICA ACTA, 1208 (339844). [PMID:35525581]
20. Su Yang, Xiaoming Tao, Wei Chen, Jianfeng Mao, Heng Luo, Shuping Lin, Lisha Zhang, Jianhua Hao. (2022) Ionic Hydrogel for Efficient and Scalable Moisture-Electric Generation. ADVANCED MATERIALS, 34 (21): (2200693). [PMID:35358352]
21. Wenquan Huang, Shiyuan Liu, Zhiqiang Li, Yingying Liu, Qiuling Xie, Yanbin Jiang. (2022) Analysis of the differences in self-assembly behaviour, molecular structure and drug delivery performance between α and β-Zein. INDUSTRIAL CROPS AND PRODUCTS, 181 (114822). [PMID:]
22. Yu Wang, Xing-yi Huang, Joshua Aheto, Yi Ren, Xiaorui Zhang, Li Wang. (2021) Novel colorimetric sensor array for Chinese rice wine evaluation based on color reactions of flavor compounds. JOURNAL OF FOOD PROCESS ENGINEERING, 44 (12): (e13889). [PMID:]
23. Yan-Qing Wang,Hong-Mei Zhang,Jian Cao. (2014-09-10) Exploring the interactions of decabrominateddiphenyl ether and tetrabromobisphenol A with human serum albumin.. Environmental toxicology and pharmacology, 38 ((2)): (595-606). [PMID:25194328]
24. Wen Sun, Hui Li, Han Wang, Shan Xiao, Jihui Wang, Liang Feng,. (2015-06-17) Sensitivity enhancement of pH indicator and its application in the evaluation of fish freshness.. Talanta, ( 127-131 ). [PMID:26078139]

References

1. Xianheng Li, Huan Li, Keqiang Lai, Junjian Miao, Jie Zhao. (2024) New natural deep eutectic solvent for vortex-assisted liquid–liquid microextraction for the determination of bisphenols in beverages. MICROCHEMICAL JOURNAL, 197 (18): (109710). [PMID:26382858]
2. Wang Tingting, Yue Xiaoju, Han Lin, Wang Jinnong, Zhang Yuzhen, Tang Xiaofeng, Wang Shifeng. (2023) Synthesis of UiO-66-NH2@SiO2 with a multistage pore structure for effective adsorption of organic pollutants. International Journal of Low-Carbon Technologies, 18 (1284-1295). [PMID:9466265]
3. Zhihui Yu, Li Ma, Binbin Liu, Wenqing Wang, Ziqi Shang, Huichao Dang, Chunyou Liu. (2023) Improvement of foaming properties of ovalbumin: Insights into the synergistic effect of preheating and high-intensity ultrasound on physicochemical properties and structure analysis. ULTRASONICS SONOCHEMISTRY, 101 (106672). [PMID:37925915]
4. Huimin Shi, Yang Li, Kailei Xu, Jun Yin. (2023) Advantages of photo-curable collagen-based cell-laden bioinks compared to methacrylated gelatin (GelMA) in digital light processing (DLP) and extrusion bioprinting. Materials Today Bio, 23 (21): (100799). [PMID:37766893]
5. Xiang-Yu Wang, Ming-Wei Zhao, Xu-Hao Wang, Peng Liu, Meng-Yao Fan, Teng Li, Zhen-Feng Ma, Ying-Jie Dai, Cai-Li Dai. (2023) Intermolecular interactions induced property improvement for clean fracturing fluid by deep eutectic solvents. Petroleum Science, [PMID:]
6. Xue Liu, Li Wang, Bowen He, Qiao Liu, He Zhu, Andrew J. Carrier, Ken D. Oakes, Xu Zhang. (2023) Mechanistic Insights into Myofibrillar Protein Oxidation by Fenton Chemistry Regulated by Gallic Acid. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 71 (33): (12587–12596). [PMID:37561819]
7. Ting Zeng, Jie Yin, Ziwen Liu, Zhaoxing Li, Yu Zhang, Yang Lv, Mei-Ling Lu, Min Luo, Meirong Chen, Yibei Xiao. (2023) Mechanistic insights into transposon cleavage and integration by TnsB of ShCAST system. Cell Reports, 42 (112698). [PMID:37379212]
8. Jie Wang, Chunyan Wu, Xing Zhang, Yuanyuan Song, Binquan Wang, Ke Zhang, Mingkuan Sun. (2023) Developmental neurotoxic effects of bisphenol A and its derivatives in Drosophila melanogaster. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 260 (115098). [PMID:37269611]
9. Guorong Ma, Pengzheng Luo, Ruiqiang Xu, Rui Ma, Lei Qiu, Chenran Xu, Rang Yang, Yating Li, Zhihao Zhao, Ling Huang, Yanhui Yang, Pei Wang. (2023) Improving the profile and reliability of cytoplasmic proteins from M. tuberculosis using biomimetic affinity chromatography coupled with LC-MS/MS analysis. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1225 (123756). [PMID:37236071]
10. Xiqing Liu, Tao Wang, Yongqing Wang. (2023) Selective and ratiometric fluorescence sensing of bisphenol A in canned food based on portable fluorescent test strips. ANALYTICA CHIMICA ACTA, 1240 (340728). [PMID:36641151]
11. Hongfeng Li, Haohao Shi, Xinyi Chen, Zhen Ren, Yu Shen, Peng Wu, Yun Fan, Xinglong Zhang, Wenxiong Shi, Honggang Liao, Suoying Zhang, Weina Zhang, Fengwei Huo. (2023) Construction of Metal–Organic Framework Films via Crosslinking-Induced Assembly. ADVANCED MATERIALS, 35 (9): (2209777). [PMID:36493462]
12. Shixin Song, Yufei Li, Qiyuan Zhu, Xin Zhang, Yang Wang, Li Tao, Lei Yu. (2023) Structure and properties of Pickering emulsions stabilized solely with novel buckwheat protein colloidal particles. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 226 (61). [PMID:36493922]
13. Qingqing Yu, Tong Shi, Zhiyu Xiong, Li Yuan, Hui Hong, Ruichang Gao, Yulong Bao. (2023) Oxidation affects dye binding of myofibrillar proteins via alteration in net charges mediated by a reduction in isoelectric point. FOOD RESEARCH INTERNATIONAL, 163 (112204). [PMID:36596136]
14. Xin Luo, Jiankang Lu, Yongyan Wu, Wenshan Duan, Fengping An, Qun Huang, Lei Chen, Shaofeng Wei. (2022) Reducing the potential allergenicity of amandin through binding to (−)-epigallocatechin gallate. Food Chemistry-X, 16 (100482). [PMID:36304206]
15. Jianchao Gong, Tingting Wang, Wei Zhang, Lin Han, Mingxiao Gao, Tianen Chen, Tao Shen, Yaxiong Ji. (2022) Organo-Vermiculites Modified by Aza-Containing Gemini Surfactants: Efficient Uptake of 2-Naphthol and Bromophenol Blue. Nanomaterials, 12 (20): (3636). [PMID:36296825]
16. Hui Liu, Mengya Li, Keyong Tang, Jie Liu, Xiumin Li, Xueping Meng. (2022) Evolution of conformation and thermal properties of bovine hides collagen in the sodium sulphide solution. JOURNAL OF MOLECULAR LIQUIDS, 367 (120449). [PMID:]
17. Lian Xia, Yuxia Yang, Hongqin Yang, Yue Tang, Jianli Zhou, Yuangen Wu. (2022) Screening and identification of an aptamer as novel recognition molecule in the test strip and its application for visual detection of ethyl carbamate in liquor. ANALYTICA CHIMICA ACTA, 1226 (340289). [PMID:36068048]
18. Hong Zeng, Hongli Yang, Yue Tang, Xiaojuan Niu, Yuangen Wu. (2022) Aptamer-enhanced the Ag(I) ion-3,3′,5,5′-tetramethylbenzdine catalytic system as a novel colorimetric biosensor for ultrasensitive and selective detection of paraquat. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 280 (121571). [PMID:35780760]
19. Zhi-Yong Wu, Yu-Hang Wang, Bing-Su Niu, Yu Yang, Fang Fang, Yan-Yan Song. (2022) Simultaneous enrichment and separation based on ion concentration polarization effect on a paper based analytical device. ANALYTICA CHIMICA ACTA, 1208 (339844). [PMID:35525581]
20. Su Yang, Xiaoming Tao, Wei Chen, Jianfeng Mao, Heng Luo, Shuping Lin, Lisha Zhang, Jianhua Hao. (2022) Ionic Hydrogel for Efficient and Scalable Moisture-Electric Generation. ADVANCED MATERIALS, 34 (21): (2200693). [PMID:35358352]
21. Wenquan Huang, Shiyuan Liu, Zhiqiang Li, Yingying Liu, Qiuling Xie, Yanbin Jiang. (2022) Analysis of the differences in self-assembly behaviour, molecular structure and drug delivery performance between α and β-Zein. INDUSTRIAL CROPS AND PRODUCTS, 181 (114822). [PMID:]
22. Yu Wang, Xing-yi Huang, Joshua Aheto, Yi Ren, Xiaorui Zhang, Li Wang. (2021) Novel colorimetric sensor array for Chinese rice wine evaluation based on color reactions of flavor compounds. JOURNAL OF FOOD PROCESS ENGINEERING, 44 (12): (e13889). [PMID:]
23. Yan-Qing Wang,Hong-Mei Zhang,Jian Cao. (2014-09-10) Exploring the interactions of decabrominateddiphenyl ether and tetrabromobisphenol A with human serum albumin.. Environmental toxicology and pharmacology, 38 ((2)): (595-606). [PMID:25194328]
24. Wen Sun, Hui Li, Han Wang, Shan Xiao, Jihui Wang, Liang Feng,. (2015-06-17) Sensitivity enhancement of pH indicator and its application in the evaluation of fish freshness.. Talanta, ( 127-131 ). [PMID:26078139]

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Bromophenol blue indicator - 0.05%（W/V）in water, high purity , CAS No.115-39-9