pAb; Rabbit anti Human Calpain 2 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab092535-10μl | 10μl | In stock | $69.90 | |
| Ab092535-50μl | 50μl | In stock | $199.90 | |
| Ab092535-100μl | 100μl | In stock | $309.90 | |
| Ab092535-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
pAb; Rabbit anti Human Calpain 2 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Calpain 2 Antibody - Primary antibody, specific to Calpain 2, Rabbit IgG |
|---|---|
| Synonyms | Calcium-activated neutral proteinase 2 | Calpain large polypeptide L2 | Calpain M-type | Calpain-2 large subunit | CANP 2 | EC 3.4.22.53 | M-calpain | Millimolar-calpain | Calcium activated neutral proteinase 2 antibody | Calcium activated neutral protein |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | Calpain 2 |
| Immunogen | Recombinant CAPN2 expressed in E.coli (AA 45-514). |
| Positive Control | WB: HeLa, A549 and HEK293 cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Rabbit anti Human Calpain 2 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified + Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Calpain 2 Antibody (Ab092535) - Western Blot
All lanes: Calpain 2 Antibody (Ab092535) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 80 kDa
Observed band size: 76 kDa
Exposure time: 52.9 s
Calpain 2 Antibody (Ab092535) - IF/ICC
FITC staining on IF
Samples: Human HeLa Cells.
Primary Ab: 10 µg/mL Calpain 2 Antibody (Ab092535).
Calpain 2 Antibody (Ab092535) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL Calpain 2 Antibody (Ab092535).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
| ICC/IF |
5-20 µg/mL
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404438 | Certificate of Analysis | Apr 11, 2024 | Ab092535 |
| ZJ24F0404437 | Certificate of Analysis | Apr 11, 2024 | Ab092535 |
| ZJ24F0404436 | Certificate of Analysis | Apr 11, 2024 | Ab092535 |
| 1. Qian J, Cuerrier D, Davies PL, Li Z, Powers JC, Campbell RL.. (2008) Cocrystal structures of primed side-extending alpha-ketoamide inhibitors reveal novel calpain-inhibitor aromatic interactions.. J Med Chem, 51 (17): (5264-5270). [PMID:18702462] |
| 2. Hata, A A, Ohno, S S, Akita, Y Y and Suzuki, K K.. (1989) Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium-dependent protease.. The Journal of biological chemistry, (15): [PMID:2539381] |
| 3. Imajoh, S S and 6 more authors.. (1988) Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease.. Biochemistry, (18): [PMID:2852952] |
| 4. Strobl, S S and 11 more authors.. (2000) The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:10639123] |
| 5. Ye, Z Z and Connor, J R JR.. (2000) cDNA cloning by amplification of circularized first strand cDNAs reveals non-IRE-regulated iron-responsive mRNAs.. Biochemical and biophysical research communications, (18): [PMID:10944468] |
| 6. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 7. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 8. Sánchez-Sánchez, Francisco F, Martínez-Redondo, Francisco F, Aroca-Aguilar, J Daniel JD, Coca-Prados, Miguel M and Escribano, Julio J.. (2007) Characterization of the intracellular proteolytic cleavage of myocilin and identification of calpain II as a myocilin-processing protease.. The Journal of biological chemistry, (21): [PMID:17650508] |
| 9. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 10. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
