Spectrophotometry is a scientific method that measures the interaction of light with matter. In particular, it's used to determine the concentration of a sample by analyzing the amount of light absorbed, transmitted, or reflected by the sample. The method can be used in the ultraviolet (UV), visible (Vis), or infrared (IR) regions of the electromagnetic spectrum. UV-visible spectrophotometry (UV-Vis) is a commonly used technique that measures the absorbance of a sample in the ultraviolet and visible regions of the spectrum. This type of spectrophotometry is used to study the molecular absorption and transmission of light in a sample and is particularly useful for identifying the presence of chromophores (substances that absorb light) in a sample. Infrared spectrophotometry (IR) is another variation of the method that measures the absorption of infrared radiation by a sample. It is used to study the vibrations and rotations of molecules in a sample and can provide information on the functional groups present in a sample. Overall, spectrophotometry is an important tool in a variety of fields, including chemistry, biology, and environmental science, and is used to determine the concentration of substances, identify the presence of specific compounds, and study the molecular structure of samples.
