The process of isolating and purifying DNA from various sources has become faster and more efficient. High yield and remnant-free DNA samples can be collected with products and kits tailored to the extraction component. Genomic DNA, cDNA, and DNA fragments can be isolated and/or purified from tissue samples, bacterial hosts, or plasmids. Over 2000 products are available for genomic DNA purification from samples like, fungus, yeast, virus, and plant in spin columns or microplate high-throughput kits. The isolation and purification systems will depend upon the downstream application including PCR or restriction digest. Column-based purification kits are also offered for cDNA samples intended for sequencing or library construction. A comprehensive product listing from an array of suppliers is presented for comparison. Isolating high-quality RNA molecules is crucial to many downstream experiments, such as cloning, reverse transcription for cDNA synthesis, RT-PCR, RT-qPCR and RNA-seq. There are various approaches to RNA purification including phenol-chloroform extraction, spin column purification, and the use of magnetic beads. Total RNA purification involves the extraction and purification of total RNA from your sample, for use in gene expression analyses such as RT-qPCR or RNA-seq. Depending on the starting sample, different protocols should be employed to most efficiently lyse cells or tissues, and purify the RNA from contamination genomic DNA and other cellular components. Theoretically, total RNA purification involves the isolation of all of the cellular RNA, including various sizes ranging from intact ribosomal RNA down to small RNA (including miRNA, tRNA and 5sRNA).
