WB: K562, HeLa, HEK293, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human CCDC47 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions. Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein (By similarity). Within the PAT subcomplex, CCDC47 occludes the lateral gate of the SEC61 complex (By similarity). Involved in the regulation of calcium ion homeostasis in the ER. Required for proper protein degradation via the ERAD (ER-associated degradation) pathway. Has an essential role in the maintenance of ER organization during embryogenesis (By similarity).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.8 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
CCDC47 Antibody (Ab093389) - Western Blot All lanes: CCDC47 Antibody (Ab093389) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 58 kDa Exposure time: 8.1 s
CCDC47 Antibody (Ab093389) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using CCDC47 Antibody (Ab093389). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets
CCDC47Tbio Coiled-coil domain-containing protein 47 0 Activities