Rat anti Human CCR5 Antibody, Monoclonal (HEK/1/85a/7a), could be used for Flow, ICC/IF and so on. Application Flow: ≤1.0µg/100w cells ICC/IF: Use at an assay dependent concentration. Protein Function Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
Product Properties
Isotype
Rat IgG2b
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
CCR5 Rat mAb (Ab093557) - Flow Cytometry Two-color flow cytometric analysis of CD19 expression on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with CD3 Mouse mAb (APC) (Ab179706) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CCR5 Rat mAb (Ab093557; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CCR5 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.
CCR5 Rat mAb (Ab093557) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD19 (red) with CCR5 Rat mAb (Ab093557) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
CCR5 Rat mAb (Ab093557) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD19 with CCR5 Rat mAb (Ab093557) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).
Associated Targets
CCR5Tclin C-C chemokine receptor type 5 0 Activities
ZJ24F0708252