Basic Description

Product Name	CD11a Mouse mAb - Primary antibody, specific to CD11a, Mouse IgG1
Synonyms	CD11 antigen-like family member A; CD11a antigen; CD11a; CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide; Integrin alpha L; integrin alpha-L; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte
Specifications & Purity	ExactAb™, Validated, Carrier Free, Lot by Lot
Host species	Mouse
Specificity	CD11a
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human CD11a Antibody, Monoclonal (HI111), could be used for Flow and so on. Application: Flow: ＜0.5µg/100w cells Protein Function Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot.

Images

CD11a Mouse mAb (Ab093706) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD11a (red) with CD11a Mouse mAb (Ab093706) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets

ITGAL Tclin Integrin alpha-L 0 Activities

Discover Target: ITGAL

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
Flow	< 0.5µg/100w cells

Certificates

Certificate of Analysis(COA)

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0102458	Certificate of Analysis	Jan 18, 2024	Ab093706
ZJ24F0102457	Certificate of Analysis	Jan 18, 2024	Ab093706
ZJ24F0102456	Certificate of Analysis	Jan 18, 2024	Ab093706

References

1. Lin Kc, Ateeq HS, Hsiung SH, Chong LT, Zimmerman CN, Castro A, Lee WC, Hammond CE, Kalkunte S, Chen LL et al.. (1999) Selective, tight-binding inhibitors of integrin alpha4beta1 that inhibit allergic airway responses.. J Med Chem, 42 (5): (920-34). [PMID:10072689]
2. Yenari MA, Kunis D, Sun GH, Onley D, Watson L, Turner S, Whitaker S, Steinberg GK. (1998) Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin, reduces injury in a rabbit model of transient focal cerebral ischemia.. Exp Neurol, 153 (2): (223-33). [PMID:9784282]
3. Burdick DJ, Paris K, Weese K, Stanley M, Beresini M, Clark K, McDowell RS, Marsters JC, Gadek TR.. (2003) N-Benzoyl amino acids as LFA-1/ICAM inhibitors 1: amino acid structure-activity relationship.. Bioorg Med Chem Lett, 13 (6): (1015-1018). [PMID:12643901]
4. Pei Z, Xin Z, Liu G, Li Y, Reilly EB, Lubbers NL, Huth JR, Link JT, von Geldern TW, Cox BF, Leitza S, Gao Y, Marsh KC, DeVries P, Okasinski GF.. (2001) Discovery of potent antagonists of leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction. 3. Amide (C-ring) structure-activity relationship and improvement of overall properties of arylthio cinnamides.. J Med Chem, 44 (18): (2913-2920). [PMID:11520199]
5. Zhong M, Shen W, Barr KJ, Arbitrario JP, Arkin MR, Bui M, Chen T, Cunningham BC, Evanchik MJ, Hanan EJ, Hoch U, Huen K, Hyde J, Kumer JL, Lac T, Lawrence CE, Martell JR, Oslob JD, Paulvannan K, Prabhu S, Silverman JA, Wright J, Yu CH, Zhu J, Flanagan WM.. (2010) Discovery of tetrahydroisoquinoline (THIQ) derivatives as potent and orally bioavailable LFA-1/ICAM-1 antagonists.. Bioorg Med Chem Lett, 20 (17): (5269-5273). [PMID:20655213]
6. San Sebastián E, Zimmerman T, Zubia A, Vara Y, Martin E, Sirockin F, Dejaegere A, Stote RH, Lopez X, Pantoja-Uceda D, Valcárcel M, Mendoza L, Vidal-Vanaclocha F, Cossío FP, Blanco FJ.. (2013) Design, synthesis, and functional evaluation of leukocyte function associated antigen-1 antagonists in early and late stages of cancer development.. J Med Chem, 56 (3): (735-747). [PMID:23339734]
7. Larson, R S RS, Corbi, A L AL, Berman, L L and Springer, T T.. (1989) Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily.. The Journal of cell biology, [PMID:2537322]
8. Qu, A A and Leahy, D J DJ.. (1995) Crystal structure of the I-domain from the CD11a/CD18 (LFA-1, alpha L beta 2) integrin.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:7479767]
9. Cornwell, R D RD, Gollahon, K A KA and Hickstein, D D DD.. (1993) Description of the leukocyte function-associated antigen 1 (LFA-1 or CD11a) promoter.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8097887]
10. Shelley, C S CS, Farokhzad, O C OC and Arnaout, M A MA.. (1993) Identification of cell-specific and developmentally regulated nuclear factors that direct myeloid and lymphoid expression of the CD11a gene.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8099450]
11. Nueda, A A, López-Cabrera, M M, Vara, A A and Corbí, A L AL.. (1993) Characterization of the CD11a (alpha L, LFA-1 alpha) integrin gene promoter.. The Journal of biological chemistry, (15): [PMID:8103515]
12. Qu, A A and Leahy, D J DJ.. (1996) The role of the divalent cation in the structure of the I domain from the CD11a/CD18 integrin.. Structure (London, England : 1993), (15): [PMID:8805579]
13. Loftus, B J BJ and 18 more authors.. (1999) Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q.. Genomics, (15): [PMID:10493829]
14. Kallen, J J and 8 more authors.. (1999) Structural basis for LFA-1 inhibition upon lovastatin binding to the CD11a I-domain.. Journal of molecular biology, (10): [PMID:10493852]
15. Weitz-Schmidt, G G and 8 more authors.. (2001) Statins selectively inhibit leukocyte function antigen-1 by binding to a novel regulatory integrin site.. Nature medicine, [PMID:11385505]
16. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ.. (2002) TTD: Therapeutic Target Database.. Nucleic acids research, (1): [PMID:11752352]
17. Ostermann, Georg G, Weber, Kim S C KS, Zernecke, Alma A, Schröder, Andreas A and Weber, Christian C.. (2002) JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes.. Nature immunology, [PMID:11812992]
18. Shimaoka, Motomu M and 11 more authors.. (2003) Structures of the alpha L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation.. Cell, (10): [PMID:12526797]
19. Katano, Harutaka H, Pesnicak, Lesley L and Cohen, Jeffrey I JI.. (2004) Simvastatin induces apoptosis of Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines and delays development of EBV lymphomas.. Proceedings of the National Academy of Sciences of the United States of America, (6): [PMID:15041742]
20. Barber, Domingo F DF, Faure, Mathias M and Long, Eric O EO.. (2004) LFA-1 contributes an early signal for NK cell cytotoxicity.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:15356110]
21. Martin, Joel J and 121 more authors.. (2004) The sequence and analysis of duplication-rich human chromosome 16.. Nature, (23): [PMID:15616553]
22. Wellington, Keri K and Perry, Caroline M CM.. (2005) Efalizumab.. American journal of clinical dermatology, [PMID:15799683]
23. Liao, James K JK and Laufs, Ulrich U.. (2005) Pleiotropic effects of statins.. Annual review of pharmacology and toxicology, [PMID:15822172]
24. Fagerholm, Susanna C SC, Hilden, Tiina J TJ, Nurmi, Susanna M SM and Gahmberg, Carl G CG.. (2005) Specific integrin alpha and beta chain phosphorylations regulate LFA-1 activation through affinity-dependent and -independent mechanisms.. The Journal of cell biology, (21): [PMID:16301335]
25. and Descamps, V V.. () [Efalizumab].. Annales de dermatologie et de venereologie, [PMID:17053736]
26. Frampton, James E JE and Plosker, Greg L GL.. (2009) Efalizumab: a review of its use in the management of chronic moderate-to-severe plaque psoriasis.. American journal of clinical dermatology, [PMID:19170413]
27. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973]
28. Kristóf, Endre E and 5 more authors.. (2013) Novel role of ICAM3 and LFA-1 in the clearance of apoptotic neutrophils by human macrophages.. Apoptosis : an international journal on programmed cell death, [PMID:23775590]
29. Kawamoto, Eiji E and 6 more authors.. (2016) LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin.. Biochemical and biophysical research communications, (13): [PMID:27055590]
30. and Paton, D M DM.. (2016) Lifitegrast: First LFA-1/ICAM-1 antagonist for treatment of dry eye disease.. Drugs of today (Barcelona, Spain : 1998), [PMID:27883115]
31. Swaim, Caleb D CD, Scott, Ariella F AF, Canadeo, Larissa A LA and Huibregtse, Jon M JM.. (2017) Extracellular ISG15 Signals Cytokine Secretion through the LFA-1 Integrin Receptor.. Molecular cell, (2): [PMID:29100055]

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SKU	Size	Availability	Price
Ab093706-10μg	10μg	In stock	$26.90
Ab093706-50μg	50μg	In stock	$69.90
Ab093706-100μg	100μg	In stock	$99.90
Ab093706-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$899.90

CD11a Mouse mAb - Primary antibody, specific to CD11a, Mouse IgG1