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CD25 Rat mAb (APC) - Primary antibody, specific to CD25, IgG1

  • Azide Free
  • ExactAb™
  • Validated
  • 5μL/test
Features and benefits
  • Short Overview:

    mAb(PC-61.5.3); Rat anti Mouse CD25 Antibody (APC); Flow

  • Species reactivity(Reacts with): Mouse
  • Isotype: IgG1
    Application:
  • Flow Cytometry
Item Number
Ab179012
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab179012-10T (Trial Size)
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10T
In stock
$39.90
Ab179012-25T
25T
In stock
$59.90
Ab179012-100T
100T
In stock
$119.90

mAb(PC-61.5.3); Rat anti Mouse CD25 Antibody (APC); Flow

Basic Description

Product NameCD25 Rat mAb (APC) - Primary antibody, specific to CD25, IgG1
Specifications & PurityExactAb™, Validated, Azide Free, 5μL/test
Host speciesRat
SpecificityCD25
ImmunogenIL-2-dependent cytolytic mouse T cell clone B6.1
Positive ControlFlow: Murine splenocytes
ConjugationAPC
GradeAzide Free, ExactAb™, Validated
Product Description

Application:
Flow: 5μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume.

Product Properties

IsotypeIgG1
Light Chain Typeλ
Purification MethodProtein A High Salt
FormLiquid
Concentration5μL/test
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

CD25 Rat mAb (APC) (Ab179012) - Flow Cytometry
BALB/c mouse splenocytes are stained with CD25 Rat mAb (APC) (Ab179012) and CD4 Rat mAb (FITC) (Ab181111) (right). BALB/c mouse splenocytes are stained with CD4 Rat mAb (FITC) (Ab181111) and Rat IgG Isotype Control Antibody (APC) (Ab180313) (left).

CD25 Rat mAb (APC) (Ab179012) - Flow Cytometry
Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (APC) (Ab179012) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).

Associated Targets

IL2RA Tclin Interleukin-2 receptor subunit alpha 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
Flow

5μl/test

Certificates

Certificate of Analysis(COA)

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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404893Certificate of AnalysisApr 25, 2024 Ab179012
ZJ24F0404892Certificate of AnalysisApr 25, 2024 Ab179012
ZJ24F0404891Certificate of AnalysisApr 25, 2024 Ab179012

Related Documents

Solution Calculators