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CD25 Rat mAb (Biotin) - Primary antibody, specific to CD25, IgG1
Specifications & Purity
ExactAb™, Validated, Azide Free, 1.0 mg/mL
Host species
Rat
Specificity
CD25
Immunogen
IL-2-dependent cytolytic mouse T cell clone B6.1
Positive Control
Flow: Murine splenocytes
Conjugation
Biotin
Grade
Azide Free, ExactAb™, Validated
Product Description
Application: Flow: ≤0.5μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.
Product Properties
Isotype
IgG1
Light Chain Type
λ
Purification Method
Protein A High Salt
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at 2-8°C,Protected from light,Freeze prohibited
Shipped In
Wet ice
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry BALB/c mouse splenocytes are stained with CD25 Rat mAb (Biotin) (Ab179014) and CD4 Rat mAb (APC) (Ab181112) (right), followed by Recombinant Streptavidin Protein (R-PE) (rp156150). BALB/c mouse splenocytes are stained with CD4 Rat mAb (APC) (Ab181112) and Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left).
CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (Biotin) (Ab179014) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).