Click Here for 5% Off Your First Aladdin Purchase!

CD25 Rat mAb (Biotin) - Primary antibody, specific to CD25, IgG1

  • Azide Free
  • ExactAb™
  • Validated
  • 1.0 mg/mL
Features and benefits
  • Short Overview:

    mAb(PC-61.5.3); Rat anti Mouse CD25 Antibody (Biotin); Flow

  • Species reactivity(Reacts with): Mouse
  • Isotype: IgG1
    Application:
  • Flow Cytometry
Item Number
Ab179014
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab179014-10μg (Trial Size)
Apply for free trial size(?)
Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10μg
In stock
$29.90
Ab179014-25μg
25μg
In stock
$49.90
Ab179014-100μg
100μg
In stock
$89.90

mAb(PC-61.5.3); Rat anti Mouse CD25 Antibody (Biotin); Flow

Basic Description

Product NameCD25 Rat mAb (Biotin) - Primary antibody, specific to CD25, IgG1
Specifications & PurityExactAb™, Validated, Azide Free, 1.0 mg/mL
Host speciesRat
SpecificityCD25
ImmunogenIL-2-dependent cytolytic mouse T cell clone B6.1
Positive ControlFlow: Murine splenocytes
ConjugationBiotin
GradeAzide Free, ExactAb™, Validated
Product Description

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Product Properties

IsotypeIgG1
Light Chain Typeλ
Purification MethodProtein A High Salt
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry
BALB/c mouse splenocytes are stained with CD25 Rat mAb (Biotin) (Ab179014) and CD4 Rat mAb (APC) (Ab181112) (right), followed by Recombinant Streptavidin Protein (R-PE) (rp156150). BALB/c mouse splenocytes are stained with CD4 Rat mAb (APC) (Ab181112) and Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left).

CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry
Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (Biotin) (Ab179014) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).

Associated Targets

IL2RA Tclin Interleukin-2 receptor subunit alpha 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
Flow

≤0.5μg/test

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404896Certificate of AnalysisApr 25, 2024 Ab179014
ZJ24F0404895Certificate of AnalysisApr 25, 2024 Ab179014
ZJ24F0404894Certificate of AnalysisApr 25, 2024 Ab179014

Related Documents

Solution Calculators