CD3 epsilon Mouse mAb - Primary antibody, specific to CD3E, Mouse IgG1
Specifications & Purity
ExactAb™, Validated, Carrier Free, High performance, Lot by Lot
Host species
Mouse
Specificity
CD3E
Immunogen
Recombinant CD3e expressed in E.coli (AA 28-185)
Positive Control
WB: Jurkat and MOLT-4 cell lysates. IHC: Human spleen tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High performance, Validated
Product Description
Mouse anti Human CD3 epsilon Antibody, Monoclonal (C10), could be used for WB, IHC and so on. Application: WB: 0.01-3µg/mL IHC: 5-20µg/mL Protein Function: The CD3 complex mediates signal transduction.
Product Properties
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
CD3 epsilon Mouse mAb (Ab094446) - Western Blot All lanes: CD3 epsilon Mouse mAb (Ab094446) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 23 kDa Observed band size: 22 kDa Exposure time: 20.0 s
CD3 epsilon Mouse mAb (Ab094446) - IHC DAB staining on IHC Sample: Human Spleen Tissue Primary Ab: 20 µg/mL CD3 epsilon Mouse mAb (Ab094446) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.