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CD3 Rat mAb (Biotin)

  • ExactAb™
  • Validated
  • 0.5 mg/mL
Features and benefits
  • Short Overview:

    mAb (17A2); Rat anti Mouse CD3 Antibody (Biotin); Flow

  • Species reactivity(Reacts with): Mouse
  • Isotype: Rat IgG2b
    Application:
  • Flow Cytometry
Item Number
Ab185405
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab185405-10μg (Trial Size)
Apply for free trial size(?)
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10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$11.90
Ab185405-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$22.90
Ab185405-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90

mAb (17A2); Rat anti Mouse CD3 Antibody (Biotin); Flow

Basic Description

Product NameCD3 Rat mAb (Biotin)
Specifications & PurityExactAb™, Validated, 0.5 mg/mL
Host speciesRat
SpecificityCD3
ImmunogenγδTCR-positive T-T hybridoma D1
Positive ControlFlow: Mouse splenocytes.
ConjugationBiotin
GradeExactAb™, Validated
Product Description

Flow: ≤0.3 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Product Properties

IsotypeRat IgG2b
Light Chain Typekappa
Purification MethodProtein G purified
Concentration0.5 mg/mL
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

CD3 Rat mAb (Biotin) (Ab185405) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Rat mAb (Biotin) (Ab185405) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

CD3 Rat mAb (Biotin) (Ab185405) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD3 Rat mAb (Biotin) (Ab185405) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets

CD3G Tclin T-cell surface glycoprotein CD3 gamma chain 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
Flow

≤0.3μg/test

Certificates

Certificate of Analysis(COA)

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4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0809130Certificate of AnalysisAug 02, 2024 Ab185405
ZJ24F0809129Certificate of AnalysisAug 02, 2024 Ab185405
ZJ24F0809131Certificate of AnalysisAug 02, 2024 Ab185405
ZJ24R0700849Certificate of AnalysisJul 26, 2024 Ab185405

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